Active focus stabilization for fluorescence microscopy by tracking actively generated infrared spots
van den Berg B, Van den Eynde R, Müller M, Vandenberg W, Dedecker P (2020)
bioRxiv.
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| Veröffentlicht | Englisch
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Autor*in
van den Berg, Birthe;
Van den Eynde, Robin;
Müller, MarcelUniBi 
;
Vandenberg, Wim;
Dedecker, Peter
;
Vandenberg, Wim;
Dedecker, PeterEinrichtung
Abstract / Bemerkung
Maintaining focus during time-lapse experiments remains an important challenge in optical microscopy. In this paper we present an automated focusing system for fluorescence microscopy that can be used in time-lapse experiments. We provide software for flexibly operating the autofocus system and a complete alignment guide. The system is evaluated using a time-lapse experiment with temperature variations on Tetraspeck beads.
Erscheinungsjahr
2020
Zeitschriftentitel
bioRxiv
Page URI
https://pub.uni-bielefeld.de/record/2967977
Zitieren
van den Berg B, Van den Eynde R, Müller M, Vandenberg W, Dedecker P. Active focus stabilization for fluorescence microscopy by tracking actively generated infrared spots. bioRxiv. 2020.
van den Berg, B., Van den Eynde, R., Müller, M., Vandenberg, W., & Dedecker, P. (2020). Active focus stabilization for fluorescence microscopy by tracking actively generated infrared spots. bioRxiv. https://doi.org/10.1101/2020.09.22.308197
van den Berg, Birthe, Van den Eynde, Robin, Müller, Marcel, Vandenberg, Wim, and Dedecker, Peter. 2020. “Active focus stabilization for fluorescence microscopy by tracking actively generated infrared spots”. bioRxiv.
van den Berg, B., Van den Eynde, R., Müller, M., Vandenberg, W., and Dedecker, P. (2020). Active focus stabilization for fluorescence microscopy by tracking actively generated infrared spots. bioRxiv.
van den Berg, B., et al., 2020. Active focus stabilization for fluorescence microscopy by tracking actively generated infrared spots. bioRxiv.
B. van den Berg, et al., “Active focus stabilization for fluorescence microscopy by tracking actively generated infrared spots”, bioRxiv, 2020.
van den Berg, B., Van den Eynde, R., Müller, M., Vandenberg, W., Dedecker, P.: Active focus stabilization for fluorescence microscopy by tracking actively generated infrared spots. bioRxiv. (2020).
van den Berg, Birthe, Van den Eynde, Robin, Müller, Marcel, Vandenberg, Wim, and Dedecker, Peter. “Active focus stabilization for fluorescence microscopy by tracking actively generated infrared spots”. bioRxiv (2020).
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