Microglial CD74 Expression Is Regulated by TGFβ Signaling
Jahn J, Bollensdorf A, Kalischer C, Piecha R, Weiß-Müller J, Potru P, Ruß T, Spittau B (2022)
International Journal of Molecular Sciences 23(18): 10247.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
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ijms-23-10247.pdf
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Autor*in
Jahn, Jannik;
Bollensdorf, Antonia;
Kalischer, Christopher;
Piecha, Robin;
Weiß-Müller, JanaUniBi;
Potru, PhaniUniBi;
Ruß, TamaraUniBi;
Spittau, BjörnUniBi
Abstract / Bemerkung
Microglia play important roles during physiological and pathological situations in the CNS. Several reports have described the expression of Cd74 in disease-associated and aged microglia. Here, we demonstrated that TGFβ1 controled the expression of Cd74 in microglia in vitro and in vivo. Using BV2 cells, primary microglia cultures as well as Cx3cr1CreERT2:R26-YFP:Tgfbr2fl/fl in combination with qPCR, flow cytometry, and immunohistochemistry, we were able to provide evidence that TGFβ1 inhibited LPS-induced upregulation of Cd74 in microglia. Interestingly, TGFβ1 alone was able to mediate downregulation of CD74 in vitro. Moreover, silencing of TGFβ signaling in vivo resulted in marked upregulation of CD74, further underlining the importance of microglial TGFβ signaling during regulation of microglia activation. Taken together, our data indicated that CD74 is a marker for activated microglia and further demonstrated that microglial TGFβ signaling is important for regulation of Cd74 expression during microglia activation.
Stichworte
CD74;
microglia;
TGFβ1;
LPS
Erscheinungsjahr
2022
Zeitschriftentitel
International Journal of Molecular Sciences
Band
23
Ausgabe
18
Art.-Nr.
10247
Urheberrecht / Lizenzen
eISSN
1422-0067
Finanzierungs-Informationen
Open-Access-Publikationskosten wurden durch die Universität Bielefeld gefördert.
Page URI
https://pub.uni-bielefeld.de/record/2965599
Zitieren
Jahn J, Bollensdorf A, Kalischer C, et al. Microglial CD74 Expression Is Regulated by TGFβ Signaling. International Journal of Molecular Sciences. 2022;23(18): 10247.
Jahn, J., Bollensdorf, A., Kalischer, C., Piecha, R., Weiß-Müller, J., Potru, P., Ruß, T., et al. (2022). Microglial CD74 Expression Is Regulated by TGFβ Signaling. International Journal of Molecular Sciences, 23(18), 10247. https://doi.org/10.3390/ijms231810247
Jahn, Jannik, Bollensdorf, Antonia, Kalischer, Christopher, Piecha, Robin, Weiß-Müller, Jana, Potru, Phani, Ruß, Tamara, and Spittau, Björn. 2022. “Microglial CD74 Expression Is Regulated by TGFβ Signaling”. International Journal of Molecular Sciences 23 (18): 10247.
Jahn, J., Bollensdorf, A., Kalischer, C., Piecha, R., Weiß-Müller, J., Potru, P., Ruß, T., and Spittau, B. (2022). Microglial CD74 Expression Is Regulated by TGFβ Signaling. International Journal of Molecular Sciences 23:10247.
Jahn, J., et al., 2022. Microglial CD74 Expression Is Regulated by TGFβ Signaling. International Journal of Molecular Sciences, 23(18): 10247.
J. Jahn, et al., “Microglial CD74 Expression Is Regulated by TGFβ Signaling”, International Journal of Molecular Sciences, vol. 23, 2022, : 10247.
Jahn, J., Bollensdorf, A., Kalischer, C., Piecha, R., Weiß-Müller, J., Potru, P., Ruß, T., Spittau, B.: Microglial CD74 Expression Is Regulated by TGFβ Signaling. International Journal of Molecular Sciences. 23, : 10247 (2022).
Jahn, Jannik, Bollensdorf, Antonia, Kalischer, Christopher, Piecha, Robin, Weiß-Müller, Jana, Potru, Phani, Ruß, Tamara, and Spittau, Björn. “Microglial CD74 Expression Is Regulated by TGFβ Signaling”. International Journal of Molecular Sciences 23.18 (2022): 10247.
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2022-09-08T07:03:22Z
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