NO Reduces PMN Adhesion to Human Vascular Endothelial Cells Due to Downregulation of ICAM-1 mRNA and Surface Expression

Lindemann S, Sharafi M, Spiecker M, Buerke M, Fisch A, Grosser T, Veit K, Gierer C, Ibe W, Meyer J, Darius H (2000)
Thrombosis Research 97(3): 113-123.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
Es wurden keine Dateien hochgeladen. Nur Publikationsnachweis!
Autor*in
Lindemann, Stephan; Sharafi, Masi; Spiecker, Martin; Buerke, Michael; Fisch, Andreas; Grosser, TiloUniBi ; Veit, Kerstin; Gierer, Claudia; Ibe, Waltraut; Meyer, Jürgen; Darius, Harald
Abstract / Bemerkung
Reperfusion damage is largely due to the adherence of polymorphonuclear leukocytes to the endothelium initiated by adhesion molecule upregulation. The reduced endothelial nitric oxide release during ischemia may be involved in the upregulation of intercellular adhesion molecule 1. In this study, we tested if nitric oxide donors suppress polymorphonuclear leukocyte adherence to activated endothelial cells by inhibition of the intercellular adhesion molecule 1 surface expression. Confluent human umbilical vein endothelial cells were stimulated with tumor necrosis factor α (300 U/mL) after preincubation with increasing concentrations of the nitric oxide donors CAS 1609 (0.005–5 mM/L) and 3-(4-morpholinyl)-sydnonimine (0.01–1 mM/L). Intercellular adhesion molecule 1 surface expression was measured in a cell surface enzyme-linked immunosorbent assay, intercellular adhesion molecule 1 mRNA by Northern analysis. Human saphenous vein endothelial cells were transfected with the inducible nitric oxide synthase gene and stimulated with tumor necrosis factor α (300 U/mL). Fluorescein green-labeled polymorphonuclear leukocytes adhering to activated human umbilical vein endothelial cells/human saphenous vein endothelial cells were quantified by epifluorescent microscopy. The intercellular adhesion molecule 1 surface expression of activated human umbilical vein endothelial cells/human saphenous vein endothelial cells was significantly diminished to 40 to 60% of the maximum after treatment with CAS 1609, 3-(4-morpholinyl)-sydnonimine, or transfection with the inducible nitric oxide synthase gene. Intercellular adhesion molecule 1 mRNA was diminished by CAS 1609 and 3-(4-morpholinyl)-sydnonimine in the same manner. The functional relevance of our data was shown by reduction of polymorphonuclear leukocyte adherence to activated human umbilical vein endothelial cells/human saphenous vein endothelial cells following treatment with CAS 1609 and 3-(4-morpholinyl)-sydnonimine or transfection with inducible nitric oxide synthase. Tumor necrosis factor-induced polymorphonuclear leukocyte adherence was abolished by blocking antibody against intercellular adhesion molecule 1. Thus, exogenous or endogenous substitution of nitric oxide diminishes the expression of endothelial intercellular adhesion molecule 1 and its mRNA following tumor necrosis factor α stimulation. This results in a reduced polymorphonuclear leukocyte adherence to activated endothelium.
Erscheinungsjahr
2000
Zeitschriftentitel
Thrombosis Research
Band
97
Ausgabe
3
Seite(n)
113-123
ISSN
00493848
Page URI
https://pub.uni-bielefeld.de/record/2965355

Zitieren

Lindemann S, Sharafi M, Spiecker M, et al. NO Reduces PMN Adhesion to Human Vascular Endothelial Cells Due to Downregulation of ICAM-1 mRNA and Surface Expression. Thrombosis Research. 2000;97(3):113-123.
Lindemann, S., Sharafi, M., Spiecker, M., Buerke, M., Fisch, A., Grosser, T., Veit, K., et al. (2000). NO Reduces PMN Adhesion to Human Vascular Endothelial Cells Due to Downregulation of ICAM-1 mRNA and Surface Expression. Thrombosis Research, 97(3), 113-123. https://doi.org/10.1016/S0049-3848(99)00162-0
Lindemann, Stephan, Sharafi, Masi, Spiecker, Martin, Buerke, Michael, Fisch, Andreas, Grosser, Tilo, Veit, Kerstin, et al. 2000. “NO Reduces PMN Adhesion to Human Vascular Endothelial Cells Due to Downregulation of ICAM-1 mRNA and Surface Expression”. Thrombosis Research 97 (3): 113-123.
Lindemann, S., Sharafi, M., Spiecker, M., Buerke, M., Fisch, A., Grosser, T., Veit, K., Gierer, C., Ibe, W., Meyer, J., et al. (2000). NO Reduces PMN Adhesion to Human Vascular Endothelial Cells Due to Downregulation of ICAM-1 mRNA and Surface Expression. Thrombosis Research 97, 113-123.
Lindemann, S., et al., 2000. NO Reduces PMN Adhesion to Human Vascular Endothelial Cells Due to Downregulation of ICAM-1 mRNA and Surface Expression. Thrombosis Research, 97(3), p 113-123.
S. Lindemann, et al., “NO Reduces PMN Adhesion to Human Vascular Endothelial Cells Due to Downregulation of ICAM-1 mRNA and Surface Expression”, Thrombosis Research, vol. 97, 2000, pp. 113-123.
Lindemann, S., Sharafi, M., Spiecker, M., Buerke, M., Fisch, A., Grosser, T., Veit, K., Gierer, C., Ibe, W., Meyer, J., Darius, H.: NO Reduces PMN Adhesion to Human Vascular Endothelial Cells Due to Downregulation of ICAM-1 mRNA and Surface Expression. Thrombosis Research. 97, 113-123 (2000).
Lindemann, Stephan, Sharafi, Masi, Spiecker, Martin, Buerke, Michael, Fisch, Andreas, Grosser, Tilo, Veit, Kerstin, Gierer, Claudia, Ibe, Waltraut, Meyer, Jürgen, and Darius, Harald. “NO Reduces PMN Adhesion to Human Vascular Endothelial Cells Due to Downregulation of ICAM-1 mRNA and Surface Expression”. Thrombosis Research 97.3 (2000): 113-123.
Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®
Quellen

PMID: 10680642
PubMed | Europe PMC

Suchen in

Google Scholar