Developmental expression of functional cyclooxygenases in zebrafish

Grosser T, Yusuff S, Cheskis E, Pack MA, FitzGerald GA (2002)
Proceedings of the National Academy of Sciences 99(12): 8418-8423.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
Es wurden keine Dateien hochgeladen. Nur Publikationsnachweis!
Autor*in
Grosser, TiloUniBi ; Yusuff, Shamila; Cheskis, Ellina; Pack, Michael A.; FitzGerald, Garret A.
Abstract / Bemerkung
Study of the cyclooxygenases (COXs) has been limited by the role of COX-2 in murine reproduction and renal organogenesis. We sought to characterize COX expression and function in zebrafish (z). Full-length cDNAs of zCOX-1 and zCOX-2 were cloned and assigned to conserved regions of chromosomes 5 and 2, respectively. The deduced proteins are 67% homologous with their human orthologs. Prostaglandin (PG) E2is the predominant zCOX product detected by mass spectrometry. Pharmacological inhibitors demonstrate selectivity when directed against heterologously expressed zCOX isoforms. Zebrafish thrombocyte aggregationex vivoand hemostasisin vivoare sensitive to inhibition of zCOX-1, but not zCOX-2. Both zCOXs were widely expressed during development, and knockdown of zCOX-1 causes growth arrest during early embryogenesis. zCOX-1 is widely evident in the embryonic vasculature, whereas zCOX-2 exhibits a more restricted pattern of expression. Both zCOX isoforms are genetically and functionally homologous to their mammalian orthologs. The zebrafish affords a tractable model system for the study of COX biology and development.
Erscheinungsjahr
2002
Zeitschriftentitel
Proceedings of the National Academy of Sciences
Band
99
Ausgabe
12
Seite(n)
8418-8423
ISSN
0027-8424
eISSN
1091-6490
Page URI
https://pub.uni-bielefeld.de/record/2965351

Zitieren

Grosser T, Yusuff S, Cheskis E, Pack MA, FitzGerald GA. Developmental expression of functional cyclooxygenases in zebrafish. Proceedings of the National Academy of Sciences. 2002;99(12):8418-8423.
Grosser, T., Yusuff, S., Cheskis, E., Pack, M. A., & FitzGerald, G. A. (2002). Developmental expression of functional cyclooxygenases in zebrafish. Proceedings of the National Academy of Sciences, 99(12), 8418-8423. https://doi.org/10.1073/pnas.112217799
Grosser, Tilo, Yusuff, Shamila, Cheskis, Ellina, Pack, Michael A., and FitzGerald, Garret A. 2002. “Developmental expression of functional cyclooxygenases in zebrafish”. Proceedings of the National Academy of Sciences 99 (12): 8418-8423.
Grosser, T., Yusuff, S., Cheskis, E., Pack, M. A., and FitzGerald, G. A. (2002). Developmental expression of functional cyclooxygenases in zebrafish. Proceedings of the National Academy of Sciences 99, 8418-8423.
Grosser, T., et al., 2002. Developmental expression of functional cyclooxygenases in zebrafish. Proceedings of the National Academy of Sciences, 99(12), p 8418-8423.
T. Grosser, et al., “Developmental expression of functional cyclooxygenases in zebrafish”, Proceedings of the National Academy of Sciences, vol. 99, 2002, pp. 8418-8423.
Grosser, T., Yusuff, S., Cheskis, E., Pack, M.A., FitzGerald, G.A.: Developmental expression of functional cyclooxygenases in zebrafish. Proceedings of the National Academy of Sciences. 99, 8418-8423 (2002).
Grosser, Tilo, Yusuff, Shamila, Cheskis, Ellina, Pack, Michael A., and FitzGerald, Garret A. “Developmental expression of functional cyclooxygenases in zebrafish”. Proceedings of the National Academy of Sciences 99.12 (2002): 8418-8423.

Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

References

Daten bereitgestellt von Europe PubMed Central.

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®
Quellen

PMID: 12011329
PubMed | Europe PMC

Suchen in

Google Scholar