EBP, a Program for Protein Identification Using Multiple Tandem Mass Spectrometry Datasets
Price TS, Lucitt MB, Wu W, Austin DJ, Pizarro A, Yocum AK, Blair IA, FitzGerald GA, Grosser T (2007)
Molecular & Cellular Proteomics 6(3): 527-536.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
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Autor*in
Price, Thomas S.;
Lucitt, Margaret B.;
Wu, Weichen;
Austin, David J.;
Pizarro, Angel;
Yocum, Anastasia K.;
Blair, Ian A.;
FitzGerald, Garret A.;
Grosser, TiloUniBi
Abstract / Bemerkung
MS/MS combined with database search methods can identify the proteins present in complex mixtures. High throughput methods that infer probable peptide sequences from enzymatically digested protein samples create a challenge in how best to aggregate the evidence for candidate proteins. Typically the results of multiple technical and/or biological replicate experiments must be combined to maximize sensitivity. We present a statistical method for estimating probabilities of protein expression that integrates peptide sequence identifications from multiple search algorithms and replicate experimental runs. The method was applied to create a repository of 797 non-homologous zebrafish (Danio rerio) proteins, at an empirically validated false identification rate under 1%, as a resource for the development of targeted quantitative proteomics assays. We have implemented this statistical method as an analytic module that can be integrated with an existing suite of open-source proteomics software.
Erscheinungsjahr
2007
Zeitschriftentitel
Molecular & Cellular Proteomics
Band
6
Ausgabe
3
Seite(n)
527-536
Urheberrecht / Lizenzen
ISSN
15359476
Page URI
https://pub.uni-bielefeld.de/record/2965342
Zitieren
Price TS, Lucitt MB, Wu W, et al. EBP, a Program for Protein Identification Using Multiple Tandem Mass Spectrometry Datasets. Molecular & Cellular Proteomics. 2007;6(3):527-536.
Price, T. S., Lucitt, M. B., Wu, W., Austin, D. J., Pizarro, A., Yocum, A. K., Blair, I. A., et al. (2007). EBP, a Program for Protein Identification Using Multiple Tandem Mass Spectrometry Datasets. Molecular & Cellular Proteomics, 6(3), 527-536. https://doi.org/10.1074/mcp.T600049-MCP200
Price, Thomas S., Lucitt, Margaret B., Wu, Weichen, Austin, David J., Pizarro, Angel, Yocum, Anastasia K., Blair, Ian A., FitzGerald, Garret A., and Grosser, Tilo. 2007. “EBP, a Program for Protein Identification Using Multiple Tandem Mass Spectrometry Datasets”. Molecular & Cellular Proteomics 6 (3): 527-536.
Price, T. S., Lucitt, M. B., Wu, W., Austin, D. J., Pizarro, A., Yocum, A. K., Blair, I. A., FitzGerald, G. A., and Grosser, T. (2007). EBP, a Program for Protein Identification Using Multiple Tandem Mass Spectrometry Datasets. Molecular & Cellular Proteomics 6, 527-536.
Price, T.S., et al., 2007. EBP, a Program for Protein Identification Using Multiple Tandem Mass Spectrometry Datasets. Molecular & Cellular Proteomics, 6(3), p 527-536.
T.S. Price, et al., “EBP, a Program for Protein Identification Using Multiple Tandem Mass Spectrometry Datasets”, Molecular & Cellular Proteomics, vol. 6, 2007, pp. 527-536.
Price, T.S., Lucitt, M.B., Wu, W., Austin, D.J., Pizarro, A., Yocum, A.K., Blair, I.A., FitzGerald, G.A., Grosser, T.: EBP, a Program for Protein Identification Using Multiple Tandem Mass Spectrometry Datasets. Molecular & Cellular Proteomics. 6, 527-536 (2007).
Price, Thomas S., Lucitt, Margaret B., Wu, Weichen, Austin, David J., Pizarro, Angel, Yocum, Anastasia K., Blair, Ian A., FitzGerald, Garret A., and Grosser, Tilo. “EBP, a Program for Protein Identification Using Multiple Tandem Mass Spectrometry Datasets”. Molecular & Cellular Proteomics 6.3 (2007): 527-536.
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