The Post-Synaptic Density of Human Postmortem Brain Tissues: An Experimental Study Paradigm for Neuropsychiatric Illnesses
Hahn C-G, Banerjee A, MacDonald ML, Cho D-S, Kamins J, Nie Z, Borgmann-Winter KE, Grosser T, Pizarro A, Ciccimaro E, Arnold SE, et al. (2009)
PLoS ONE 4(4): e5251.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
Download
Es wurden keine Dateien hochgeladen. Nur Publikationsnachweis!
Autor*in
Hahn, Chang-Gyu;
Banerjee, Anamika;
MacDonald, Matthew L.;
Cho, Dan-Sung;
Kamins, Joshua;
Nie, Zhiping;
Borgmann-Winter, Karin E.;
Grosser, TiloUniBi
;
Pizarro, Angel;
Ciccimaro, Eugene;
Arnold, Steven E.;
Wang, Hoau-Yan
Alle
![](https://pub.uni-bielefeld.de/images/icon_orcid.png)
Alle
Abstract / Bemerkung
Recent molecular genetics studies have suggested various trans-synaptic processes for pathophysiologic mechanisms of neuropsychiatric illnesses. Examination of pre- and post-synaptic scaffolds in the brains of patients would greatly aid further investigation, yet such an approach in human postmortem tissue has yet to be tested. We have examined three methods using density gradient based purification of synaptosomes followed by detergent extraction (Method 1) and the pH based differential extraction of synaptic membranes (Methods 2 and 3). All three methods separated fractions from human postmortem brains that were highly enriched in typical PSD proteins, almost to the exclusion of pre-synaptic proteins. We examined these fractions using electron microscopy (EM) and verified the integrity of the synaptic membrane and PSD fractions derived from human postmortem brain tissues. We analyzed protein composition of the PSD fractions using two dimensional liquid chromatography tandem mass spectrometry (2D LC-MS/MS) and observed known PSD proteins by mass spectrometry. Immunoprecipitation and immunoblot studies revealed that expected protein-protein interactions and certain posttranscriptional modulations were maintained in PSD fractions. Our results demonstrate that PSD fractions can be isolated from human postmortem brain tissues with a reasonable degree of integrity. This approach may foster novel postmortem brain research paradigms in which the stoichiometry and protein composition of specific microdomains are examined.
Erscheinungsjahr
2009
Zeitschriftentitel
PLoS ONE
Band
4
Ausgabe
4
Art.-Nr.
e5251
eISSN
1932-6203
Page URI
https://pub.uni-bielefeld.de/record/2965336
Zitieren
Hahn C-G, Banerjee A, MacDonald ML, et al. The Post-Synaptic Density of Human Postmortem Brain Tissues: An Experimental Study Paradigm for Neuropsychiatric Illnesses. PLoS ONE. 2009;4(4): e5251.
Hahn, C. - G., Banerjee, A., MacDonald, M. L., Cho, D. - S., Kamins, J., Nie, Z., Borgmann-Winter, K. E., et al. (2009). The Post-Synaptic Density of Human Postmortem Brain Tissues: An Experimental Study Paradigm for Neuropsychiatric Illnesses. PLoS ONE, 4(4), e5251. https://doi.org/10.1371/journal.pone.0005251
Hahn, Chang-Gyu, Banerjee, Anamika, MacDonald, Matthew L., Cho, Dan-Sung, Kamins, Joshua, Nie, Zhiping, Borgmann-Winter, Karin E., et al. 2009. “The Post-Synaptic Density of Human Postmortem Brain Tissues: An Experimental Study Paradigm for Neuropsychiatric Illnesses”. PLoS ONE 4 (4): e5251.
Hahn, C. - G., Banerjee, A., MacDonald, M. L., Cho, D. - S., Kamins, J., Nie, Z., Borgmann-Winter, K. E., Grosser, T., Pizarro, A., Ciccimaro, E., et al. (2009). The Post-Synaptic Density of Human Postmortem Brain Tissues: An Experimental Study Paradigm for Neuropsychiatric Illnesses. PLoS ONE 4:e5251.
Hahn, C.-G., et al., 2009. The Post-Synaptic Density of Human Postmortem Brain Tissues: An Experimental Study Paradigm for Neuropsychiatric Illnesses. PLoS ONE, 4(4): e5251.
C.-G. Hahn, et al., “The Post-Synaptic Density of Human Postmortem Brain Tissues: An Experimental Study Paradigm for Neuropsychiatric Illnesses”, PLoS ONE, vol. 4, 2009, : e5251.
Hahn, C.-G., Banerjee, A., MacDonald, M.L., Cho, D.-S., Kamins, J., Nie, Z., Borgmann-Winter, K.E., Grosser, T., Pizarro, A., Ciccimaro, E., Arnold, S.E., Wang, H.-Y., Blair, I.A.: The Post-Synaptic Density of Human Postmortem Brain Tissues: An Experimental Study Paradigm for Neuropsychiatric Illnesses. PLoS ONE. 4, : e5251 (2009).
Hahn, Chang-Gyu, Banerjee, Anamika, MacDonald, Matthew L., Cho, Dan-Sung, Kamins, Joshua, Nie, Zhiping, Borgmann-Winter, Karin E., Grosser, Tilo, Pizarro, Angel, Ciccimaro, Eugene, Arnold, Steven E., Wang, Hoau-Yan, and Blair, Ian A. “The Post-Synaptic Density of Human Postmortem Brain Tissues: An Experimental Study Paradigm for Neuropsychiatric Illnesses”. PLoS ONE 4.4 (2009): e5251.
Daten bereitgestellt von European Bioinformatics Institute (EBI)
Zitationen in Europe PMC
Daten bereitgestellt von Europe PubMed Central.
References
Daten bereitgestellt von Europe PubMed Central.
Export
Markieren/ Markierung löschen
Markierte Publikationen
Web of Science
Dieser Datensatz im Web of Science®Quellen
PMID: 19370153
PubMed | Europe PMC
Suchen in