Phased grapevine genome sequence assembly of an Rpv12 carrier for exploration of Rpv12 associated positional and functional Plasmopara viticola resistance genes
Frommer B, Muellner S, Holtgräwe D, Viehöver P, Huettel B, Toepfer R, Weisshaar B, Zyprian EM (2023)
Frontiers in Plant Science 14: 1180982.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
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fpls-14-1180982.pdf
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Autor*in
Frommer, BiancaUniBi 
;
Muellner, Sophia;
Holtgräwe, DanielaUniBi ;
Viehöver, PriscaUniBi;
Huettel, Bruno;
Toepfer, Reinhard;
Weisshaar, BerndUniBi ;
Zyprian, Eva M
;
Muellner, Sophia;
Holtgräwe, DanielaUniBi ;
Viehöver, PriscaUniBi;
Huettel, Bruno;
Toepfer, Reinhard;
Weisshaar, BerndUniBi ;
Zyprian, Eva MEinrichtung
Abstract / Bemerkung
The downy mildew disease caused by the oomycete Plasmopara viticola is a serious threat for grapevine and can cause enormous yield losses in viticulture. The quantitative trait locus Rpv12, mediating resistance against P. viticola, was originally found in Asian Vitis amurensis. This locus and its genes were analyzed here in detail. A haplotype-separated genome sequence of the diploid Rpv12-carrier Gf.99-03 was created and annotated. The defense response against P. viticola was investigated in an infection time-course RNA-seq experiment, revealing approximately 600 upregulated Vitis genes during host–pathogen interaction. The Rpv12 regions of the resistance and the sensitivity encoding Gf.99-03 haplotype were structurally and functionally compared with each other. Two different clusters of resistance-related genes were identified within the Rpv12 locus. One cluster carries a set of four differentially expressed genes with three ACCELERATED CELL DEATH 6-like genes. The other cluster carries a set of six resistance gene analogs related to qualitative pathogen resistance. The Rpv12 locus and its candidate genes for P. viticola resistance provide a precious genetic resource for P. viticola resistance breeding. Newly developed co-segregating simple sequence repeat markers in close proximity to the R-genes enable its improved applicability in marker-assisted grapevine breeding.
Stichworte
QTL;
resistance genes;
fully-phased genome assembly;
trio binning;
Vitis spec.;
time course infection experiment;
plant defense
Erscheinungsjahr
2023
Zeitschriftentitel
Frontiers in Plant Science
Band
14
Art.-Nr.
1180982
Urheberrecht / Lizenzen
eISSN
1664-462X
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Open-Access-Publikationskosten wurden durch die Universität Bielefeld gefördert.
Page URI
https://pub.uni-bielefeld.de/record/2964982
Zitieren
Frommer B, Muellner S, Holtgräwe D, et al. Phased grapevine genome sequence assembly of an Rpv12 carrier for exploration of Rpv12 associated positional and functional Plasmopara viticola resistance genes. Frontiers in Plant Science. 2023;14: 1180982.
Frommer, B., Muellner, S., Holtgräwe, D., Viehöver, P., Huettel, B., Toepfer, R., Weisshaar, B., et al. (2023). Phased grapevine genome sequence assembly of an Rpv12 carrier for exploration of Rpv12 associated positional and functional Plasmopara viticola resistance genes. Frontiers in Plant Science, 14, 1180982. https://doi.org/10.3389/fpls.2023.1180982
Frommer, Bianca, Muellner, Sophia, Holtgräwe, Daniela, Viehöver, Prisca, Huettel, Bruno, Toepfer, Reinhard, Weisshaar, Bernd, and Zyprian, Eva M. 2023. “Phased grapevine genome sequence assembly of an Rpv12 carrier for exploration of Rpv12 associated positional and functional Plasmopara viticola resistance genes”. Frontiers in Plant Science 14: 1180982.
Frommer, B., Muellner, S., Holtgräwe, D., Viehöver, P., Huettel, B., Toepfer, R., Weisshaar, B., and Zyprian, E. M. (2023). Phased grapevine genome sequence assembly of an Rpv12 carrier for exploration of Rpv12 associated positional and functional Plasmopara viticola resistance genes. Frontiers in Plant Science 14:1180982.
Frommer, B., et al., 2023. Phased grapevine genome sequence assembly of an Rpv12 carrier for exploration of Rpv12 associated positional and functional Plasmopara viticola resistance genes. Frontiers in Plant Science, 14: 1180982.
B. Frommer, et al., “Phased grapevine genome sequence assembly of an Rpv12 carrier for exploration of Rpv12 associated positional and functional Plasmopara viticola resistance genes”, Frontiers in Plant Science, vol. 14, 2023, : 1180982.
Frommer, B., Muellner, S., Holtgräwe, D., Viehöver, P., Huettel, B., Toepfer, R., Weisshaar, B., Zyprian, E.M.: Phased grapevine genome sequence assembly of an Rpv12 carrier for exploration of Rpv12 associated positional and functional Plasmopara viticola resistance genes. Frontiers in Plant Science. 14, : 1180982 (2023).
Frommer, Bianca, Muellner, Sophia, Holtgräwe, Daniela, Viehöver, Prisca, Huettel, Bruno, Toepfer, Reinhard, Weisshaar, Bernd, and Zyprian, Eva M. “Phased grapevine genome sequence assembly of an Rpv12 carrier for exploration of Rpv12 associated positional and functional Plasmopara viticola resistance genes”. Frontiers in Plant Science 14 (2023): 1180982.
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Phase-separated genome sequence assembly of the grapevine cultivar Gf.99-03 and its annotation
Frommer B, Müllner S, Weisshaar B, Holtgräwe D (2022)
Bielefeld University.
Frommer B, Müllner S, Weisshaar B, Holtgräwe D (2022)
Bielefeld University.
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PMID: 37223784
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Preprint: 10.1101/2022.08.06.503030
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