Phased grapevine genome sequence assembly of an Rpv12 carrier for exploration of Rpv12 associated positional and functional Plasmopara viticola resistance genes
Frommer B, Muellner S, Holtgräwe D, Viehöver P, Huettel B, Toepfer R, Weisshaar B, Zyprian EM (2023)
Frontiers in Plant Science 14: 1180982.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
Download
fpls-14-1180982.pdf
4.73 MB
Autor*in
Frommer, BiancaUniBi 
;
Muellner, Sophia;
Holtgräwe, DanielaUniBi ;
Viehöver, PriscaUniBi;
Huettel, Bruno;
Toepfer, Reinhard;
Weisshaar, BerndUniBi ;
Zyprian, Eva M
;
Muellner, Sophia;
Holtgräwe, DanielaUniBi ;
Viehöver, PriscaUniBi;
Huettel, Bruno;
Toepfer, Reinhard;
Weisshaar, BerndUniBi ;
Zyprian, Eva MEinrichtung
Abstract / Bemerkung
The downy mildew disease caused by the oomycete Plasmopara viticola is a serious threat for grapevine and can cause enormous yield losses in viticulture. The quantitative trait locus Rpv12, mediating resistance against P. viticola, was originally found in Asian Vitis amurensis. This locus and its genes were analyzed here in detail. A haplotype-separated genome sequence of the diploid Rpv12-carrier Gf.99-03 was created and annotated. The defense response against P. viticola was investigated in an infection time-course RNA-seq experiment, revealing approximately 600 upregulated Vitis genes during host–pathogen interaction. The Rpv12 regions of the resistance and the sensitivity encoding Gf.99-03 haplotype were structurally and functionally compared with each other. Two different clusters of resistance-related genes were identified within the Rpv12 locus. One cluster carries a set of four differentially expressed genes with three ACCELERATED CELL DEATH 6-like genes. The other cluster carries a set of six resistance gene analogs related to qualitative pathogen resistance. The Rpv12 locus and its candidate genes for P. viticola resistance provide a precious genetic resource for P. viticola resistance breeding. Newly developed co-segregating simple sequence repeat markers in close proximity to the R-genes enable its improved applicability in marker-assisted grapevine breeding.
Stichworte
QTL;
resistance genes;
fully-phased genome assembly;
trio binning;
Vitis spec.;
time course infection experiment;
plant defense
Erscheinungsjahr
2023
Zeitschriftentitel
Frontiers in Plant Science
Band
14
Art.-Nr.
1180982
Urheberrecht / Lizenzen
eISSN
1664-462X
Finanzierungs-Informationen
Open-Access-Publikationskosten wurden durch die Universität Bielefeld gefördert.
Page URI
https://pub.uni-bielefeld.de/record/2964982
Zitieren
Frommer B, Muellner S, Holtgräwe D, et al. Phased grapevine genome sequence assembly of an Rpv12 carrier for exploration of Rpv12 associated positional and functional Plasmopara viticola resistance genes. Frontiers in Plant Science. 2023;14: 1180982.
Frommer, B., Muellner, S., Holtgräwe, D., Viehöver, P., Huettel, B., Toepfer, R., Weisshaar, B., et al. (2023). Phased grapevine genome sequence assembly of an Rpv12 carrier for exploration of Rpv12 associated positional and functional Plasmopara viticola resistance genes. Frontiers in Plant Science, 14, 1180982. https://doi.org/10.3389/fpls.2023.1180982
Frommer, Bianca, Muellner, Sophia, Holtgräwe, Daniela, Viehöver, Prisca, Huettel, Bruno, Toepfer, Reinhard, Weisshaar, Bernd, and Zyprian, Eva M. 2023. “Phased grapevine genome sequence assembly of an Rpv12 carrier for exploration of Rpv12 associated positional and functional Plasmopara viticola resistance genes”. Frontiers in Plant Science 14: 1180982.
Frommer, B., Muellner, S., Holtgräwe, D., Viehöver, P., Huettel, B., Toepfer, R., Weisshaar, B., and Zyprian, E. M. (2023). Phased grapevine genome sequence assembly of an Rpv12 carrier for exploration of Rpv12 associated positional and functional Plasmopara viticola resistance genes. Frontiers in Plant Science 14:1180982.
Frommer, B., et al., 2023. Phased grapevine genome sequence assembly of an Rpv12 carrier for exploration of Rpv12 associated positional and functional Plasmopara viticola resistance genes. Frontiers in Plant Science, 14: 1180982.
B. Frommer, et al., “Phased grapevine genome sequence assembly of an Rpv12 carrier for exploration of Rpv12 associated positional and functional Plasmopara viticola resistance genes”, Frontiers in Plant Science, vol. 14, 2023, : 1180982.
Frommer, B., Muellner, S., Holtgräwe, D., Viehöver, P., Huettel, B., Toepfer, R., Weisshaar, B., Zyprian, E.M.: Phased grapevine genome sequence assembly of an Rpv12 carrier for exploration of Rpv12 associated positional and functional Plasmopara viticola resistance genes. Frontiers in Plant Science. 14, : 1180982 (2023).
Frommer, Bianca, Muellner, Sophia, Holtgräwe, Daniela, Viehöver, Prisca, Huettel, Bruno, Toepfer, Reinhard, Weisshaar, Bernd, and Zyprian, Eva M. “Phased grapevine genome sequence assembly of an Rpv12 carrier for exploration of Rpv12 associated positional and functional Plasmopara viticola resistance genes”. Frontiers in Plant Science 14 (2023): 1180982.
Alle Dateien verfügbar unter der/den folgenden Lizenz(en):
Creative Commons Namensnennung 4.0 International Public License (CC-BY 4.0):
Volltext(e)
Name
fpls-14-1180982.pdf
4.73 MB
Access Level
Open Access
Zuletzt Hochgeladen
2023-05-25T10:00:48Z
MD5 Prüfsumme
553e0561e421bd2e96c3787c246c79e7
Link(s) zu Volltext(en)
Access Level
Open Access
EBI - ENA Project
Material in PUB:
Publikation, die diesen PUB Eintrag enthält
Phase-separated genome sequence assembly of the grapevine cultivar Gf.99-03 and its annotation
Frommer B, Müllner S, Weisshaar B, Holtgräwe D (2022)
Bielefeld University.
Frommer B, Müllner S, Weisshaar B, Holtgräwe D (2022)
Bielefeld University.
Export
Markieren/ Markierung löschen
Markierte Publikationen
Web of Science
Dieser Datensatz im Web of Science®Quellen
PMID: 37223784
PubMed | Europe PMC
Preprint: 10.1101/2022.08.06.503030
Suchen in
Google Scholar