Unique metabolism of different glucosinolates in larvae and adults of a leaf beetle specialised on Brassicaceae
Friedrichs J, Schweiger R, Müller C (2022)
Scientific Reports 12(1): 10905.
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Einrichtung
Abstract / Bemerkung
Brassicaceae plants contain glucosinolates, which are hydrolysed by myrosinases to toxic products such as isothiocyanates and nitriles, acting as defences. Herbivores have evolved various detoxification strategies, which are reviewed here. Larvae of Phaedon cochleariae (Coleoptera: Chrysomelidae) metabolise hydrolysis products of benzenic glucosinolates by conjugation with aspartic acid. In this study, we investigated whether P. cochleariae uses the same metabolic pathway for structurally different glucosinolates, whether the metabolism differs between adults and larvae and which hydrolysis products are formed as intermediates. Feeding experiments were performed with leaves of watercress (Nasturtium officinale, Brassicaceae) and pea (Pisum sativum, non-Brassicaceae), to which glucosinolates with structurally different side chains (benzenic, indole or aliphatic) or their hydrolysis products were applied. Samples were analysed by UHPLC-QTOF-MS/MS or TD–GC–MS. The same aspartic acid conjugates as previously identified in larvae were also detected as major metabolites of benzenic glucosinolates in adults. Indol-3-ylmethyl glucosinolate was mainly metabolised to N-(1H-indol-3-ylcarbonyl) glutamic acid in adults and larvae, while the metabolism of 2-propenyl glucosinolate remains unclear. The metabolism may thus proceed primarily via isothiocyanates rather than via nitriles, while the hydrolysis occurs independently of plant myrosinases. A detoxification by conjugation with these amino acids is not yet known from other Brassicaceae-feeders.
Erscheinungsjahr
2022
Zeitschriftentitel
Scientific Reports
Band
12
Ausgabe
1
Art.-Nr.
10905
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eISSN
2045-2322
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Open-Access-Publikationskosten wurden durch die Universität Bielefeld im Rahmen des DEAL-Vertrags gefördert.
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https://pub.uni-bielefeld.de/record/2964159
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Friedrichs J, Schweiger R, Müller C. Unique metabolism of different glucosinolates in larvae and adults of a leaf beetle specialised on Brassicaceae. Scientific Reports. 2022;12(1): 10905.
Friedrichs, J., Schweiger, R., & Müller, C. (2022). Unique metabolism of different glucosinolates in larvae and adults of a leaf beetle specialised on Brassicaceae. Scientific Reports, 12(1), 10905. https://doi.org/10.1038/s41598-022-14636-6
Friedrichs, Jeanne, Schweiger, Rabea, and Müller, Caroline. 2022. “Unique metabolism of different glucosinolates in larvae and adults of a leaf beetle specialised on Brassicaceae”. Scientific Reports 12 (1): 10905.
Friedrichs, J., Schweiger, R., and Müller, C. (2022). Unique metabolism of different glucosinolates in larvae and adults of a leaf beetle specialised on Brassicaceae. Scientific Reports 12:10905.
Friedrichs, J., Schweiger, R., & Müller, C., 2022. Unique metabolism of different glucosinolates in larvae and adults of a leaf beetle specialised on Brassicaceae. Scientific Reports, 12(1): 10905.
J. Friedrichs, R. Schweiger, and C. Müller, “Unique metabolism of different glucosinolates in larvae and adults of a leaf beetle specialised on Brassicaceae”, Scientific Reports, vol. 12, 2022, : 10905.
Friedrichs, J., Schweiger, R., Müller, C.: Unique metabolism of different glucosinolates in larvae and adults of a leaf beetle specialised on Brassicaceae. Scientific Reports. 12, : 10905 (2022).
Friedrichs, Jeanne, Schweiger, Rabea, and Müller, Caroline. “Unique metabolism of different glucosinolates in larvae and adults of a leaf beetle specialised on Brassicaceae”. Scientific Reports 12.1 (2022): 10905.
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2022-10-28T10:11:29Z
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Material in PUB:
Dissertation, die diesen PUB Eintrag enthält
Detoxification of the glucosinolate-myrosinase system by generalist and specialist leaf beetles
Friedrichs J (2022)
Bielefeld: Universität Bielefeld.
Friedrichs J (2022)
Bielefeld: Universität Bielefeld.
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