L-Serine biosensor-controlled fermentative production of L-tryptophan derivatives by Corynebacterium glutamicum
Ferrer L, Elsaraf M, Mindt M, Wendisch VF (2022)
Biology 11(5): 744.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
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Abstract / Bemerkung
l-Tryptophan derivatives, such as hydroxylated or halogenated l-tryptophans, are used in therapeutic peptides and agrochemicals and as precursors of bioactive compounds, such as serotonin. l-Tryptophan biosynthesis depends on another proteinogenic amino acid, l-serine, which is condensed with indole-3-glycerophosphate by tryptophan synthase. This enzyme is composed of the α-subunit TrpA, which catalyzes the retro-aldol cleavage of indole-3-glycerol phosphate, yielding glyceraldehyde-3-phosphate and indole, and the β-subunit TrpB that catalyzes the β-substitution reaction between indole and l-serine to water and l-tryptophan. TrpA is reported as an allosteric actuator, and its absence severely attenuates TrpB activity. In this study, however, we showed that Corynebacterium glutamicum TrpB is catalytically active in the absence of TrpA. Overexpression of C. glutamicumtrpB in a trpBA double deletion mutant supported growth in minimal medium only when exogenously added indole was taken up into the cell and condensed with intracellularly synthesized l-serine. The fluorescence reporter gene of an l-serine biosensor, which was based on the endogenous transcriptional activator SerR and its target promoter PserE, was replaced by trpB. This allowed for l-serine-dependent expression of trpB in an l-serine-producing strain lacking TrpA. Upon feeding of the respective indole derivatives, this strain produced the l-tryptophan derivatives 5-hydroxytryptophan, 7-bromotryptophan, and 5-fluorotryptophan
Stichworte
Corynebacterium glutamicum;
metabolic engineering;
biosensor;
tryptophan;
hydroxytryptophan;
bromotryptophan;
fluorotryptophan;
indoles;
TrpB
Erscheinungsjahr
2022
Zeitschriftentitel
Biology
Band
11
Ausgabe
5
Art.-Nr.
744
Urheberrecht / Lizenzen
eISSN
2079-7737
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Open-Access-Publikationskosten wurden durch die Universität Bielefeld gefördert.
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https://pub.uni-bielefeld.de/record/2962839
Zitieren
Ferrer L, Elsaraf M, Mindt M, Wendisch VF. L-Serine biosensor-controlled fermentative production of L-tryptophan derivatives by Corynebacterium glutamicum. Biology. 2022;11(5): 744.
Ferrer, L., Elsaraf, M., Mindt, M., & Wendisch, V. F. (2022). L-Serine biosensor-controlled fermentative production of L-tryptophan derivatives by Corynebacterium glutamicum. Biology, 11(5), 744. https://doi.org/10.3390/biology11050744
Ferrer, Lenny, Elsaraf, Mahmoud, Mindt, Melanie, and Wendisch, Volker F. 2022. “L-Serine biosensor-controlled fermentative production of L-tryptophan derivatives by Corynebacterium glutamicum”. Biology 11 (5): 744.
Ferrer, L., Elsaraf, M., Mindt, M., and Wendisch, V. F. (2022). L-Serine biosensor-controlled fermentative production of L-tryptophan derivatives by Corynebacterium glutamicum. Biology 11:744.
Ferrer, L., et al., 2022. L-Serine biosensor-controlled fermentative production of L-tryptophan derivatives by Corynebacterium glutamicum. Biology, 11(5): 744.
L. Ferrer, et al., “L-Serine biosensor-controlled fermentative production of L-tryptophan derivatives by Corynebacterium glutamicum”, Biology, vol. 11, 2022, : 744.
Ferrer, L., Elsaraf, M., Mindt, M., Wendisch, V.F.: L-Serine biosensor-controlled fermentative production of L-tryptophan derivatives by Corynebacterium glutamicum. Biology. 11, : 744 (2022).
Ferrer, Lenny, Elsaraf, Mahmoud, Mindt, Melanie, and Wendisch, Volker F. “L-Serine biosensor-controlled fermentative production of L-tryptophan derivatives by Corynebacterium glutamicum”. Biology 11.5 (2022): 744.
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Teil dieser Dissertation
Synthetic biology-enabled production of L-tryptophan and its derivatives by Corynebacterium glutamicum
Ferrer L (2022)
Bielefeld: Universität Bielefeld.
Ferrer L (2022)
Bielefeld: Universität Bielefeld.
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