Rational Engineering of Non-Ubiquinone Containing Corynebacterium glutamicum for Enhanced Coenzyme Q10 Production

Burgardt A, Pelosi L, Hajj Chehade M, Wendisch VF, Pierrel F (2022)
Metabolites 12(5): 428.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Burgardt, ArthurUniBi; Pelosi, Ludovic; Hajj Chehade , Mahmoud; Wendisch, Volker F.UniBi ; Pierrel, Fabien
Abstract / Bemerkung
Coenzyme Q10 (CoQ10) is a lipid-soluble compound with important physiological functions and is sought after in the food and cosmetic industries owing to its antioxidant properties. In our previous proof of concept, we engineered for CoQ10 biosynthesis the industrially relevant Corynebacterium glutamicum, which does not naturally synthesize any CoQ. Here, liquid chromatography–mass spectrometry (LC–MS) analysis identified two metabolic bottlenecks in the CoQ10 production, i.e., low conversion of the intermediate 10-prenylphenol (10P-Ph) to CoQ10 and the accumulation of isoprenologs with prenyl chain lengths of not only 10, but also 8 to 11 isopentenyl units. To overcome these limitations, the strain was engineered for expression of the Ubi complex accessory factors UbiJ and UbiK from Escherichia coli to increase flux towards CoQ10, and by replacement of the native polyprenyl diphosphate synthase IspB with a decaprenyl diphosphate synthase (DdsA) to select for prenyl chains with 10 isopentenyl units. The best strain UBI6-Rs showed a seven-fold increased CoQ10 content and eight-fold increased CoQ10 titer compared to the initial strain UBI4-Pd, while the abundance of CoQ8, CoQ9, and CoQ11 was significantly reduced. This study demonstrates the application of the recent insight into CoQ biosynthesis to improve metabolic engineering of a heterologous CoQ10 production strain.
Stichworte
coenzyme Q10 (CoQ10); ubiquinone; Corynebacterium glutamicum; metabolic engineering; Ubi complex; polyprenyl diphosphate synthas
Erscheinungsjahr
2022
Zeitschriftentitel
Metabolites
Band
12
Ausgabe
5
Art.-Nr.
428
eISSN
2218-1989
Finanzierungs-Informationen
Open-Access-Publikationskosten wurden durch die Universität Bielefeld gefördert.
Page URI
https://pub.uni-bielefeld.de/record/2962833

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Burgardt A, Pelosi L, Hajj Chehade M, Wendisch VF, Pierrel F. Rational Engineering of Non-Ubiquinone Containing Corynebacterium glutamicum for Enhanced Coenzyme Q10 Production. Metabolites. 2022;12(5): 428.
Burgardt, A., Pelosi, L., Hajj Chehade , M., Wendisch, V. F., & Pierrel, F. (2022). Rational Engineering of Non-Ubiquinone Containing Corynebacterium glutamicum for Enhanced Coenzyme Q10 Production. Metabolites, 12(5), 428. https://doi.org/10.3390/metabo12050428
Burgardt, A., Pelosi, L., Hajj Chehade , M., Wendisch, V. F., and Pierrel, F. (2022). Rational Engineering of Non-Ubiquinone Containing Corynebacterium glutamicum for Enhanced Coenzyme Q10 Production. Metabolites 12:428.
Burgardt, A., et al., 2022. Rational Engineering of Non-Ubiquinone Containing Corynebacterium glutamicum for Enhanced Coenzyme Q10 Production. Metabolites, 12(5): 428.
A. Burgardt, et al., “Rational Engineering of Non-Ubiquinone Containing Corynebacterium glutamicum for Enhanced Coenzyme Q10 Production”, Metabolites, vol. 12, 2022, : 428.
Burgardt, A., Pelosi, L., Hajj Chehade , M., Wendisch, V.F., Pierrel, F.: Rational Engineering of Non-Ubiquinone Containing Corynebacterium glutamicum for Enhanced Coenzyme Q10 Production. Metabolites. 12, : 428 (2022).
Burgardt, Arthur, Pelosi, Ludovic, Hajj Chehade , Mahmoud, Wendisch, Volker F., and Pierrel, Fabien. “Rational Engineering of Non-Ubiquinone Containing Corynebacterium glutamicum for Enhanced Coenzyme Q10 Production”. Metabolites 12.5 (2022): 428.
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2022-05-11T07:12:08Z
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