A Second Gamma-Glutamylpolyamine Synthetase, GlnA2, Is Involved in Polyamine Catabolism in Streptomyces coelicolor

Krysenko S, Okoniewski N, Nentwich M, Matthews A, Bauerle M, Zinser A, Busche T, Kulik A, Gursch S, Kemeny A, Bera A, et al. (2022)
International journal of molecular sciences 23(7): 3752.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
Es wurden keine Dateien hochgeladen. Nur Publikationsnachweis!
Autor*in
Krysenko, Sergii; Okoniewski, Nicole; Nentwich, Merle; Matthews, Arne; Bauerle, Moritz; Zinser, Alina; Busche, TobiasUniBi; Kulik, Andreas; Gursch, Stephanie; Kemeny, Annika; Bera, Agnieszka; Wohlleben, Wolfgang
Alle
Abstract / Bemerkung
Streptomyces coelicolor is a soil bacterium living in a habitat with very changeable nutrient availability. This organism possesses a complex nitrogen metabolism and is able to utilize the polyamines putrescine, cadaverine, spermidine, and spermine and the monoamine ethanolamine. We demonstrated that GlnA2 (SCO2241) facilitates S. coelicolor to survive under high toxic polyamine concentrations. GlnA2 is a gamma-glutamylpolyamine synthetase, an enzyme catalyzing the first step in polyamine catabolism. The role of GlnA2 was confirmed in phenotypical studies with a glnA2 deletion mutant as well as in transcriptional and biochemical analyses. Among all GS-like enzymes in S. coelicolor, GlnA2 possesses the highest specificity towards short-chain polyamines (putrescine and cadaverine), while its functional homolog GlnA3 (SCO6962) prefers long-chain polyamines (spermidine and spermine) and GlnA4 (SCO1613) accepts only monoamines. The genome-wide RNAseq analysis in the presence of the polyamines putrescine, cadaverine, spermidine, or spermine revealed indication of the occurrence of different routes for polyamine catabolism in S. coelicolor involving GlnA2 and GlnA3. Furthermore, GlnA2 and GlnA3 are differently regulated. From our results, we can propose a complemented model of polyamine catabolism in S. coelicolor, which involves the gamma-glutamylation pathway as well as other alternative utilization pathways.
Erscheinungsjahr
2022
Zeitschriftentitel
International journal of molecular sciences
Band
23
Ausgabe
7
Art.-Nr.
3752
eISSN
1422-0067
Page URI
https://pub.uni-bielefeld.de/record/2962520

Zitieren

Krysenko S, Okoniewski N, Nentwich M, et al. A Second Gamma-Glutamylpolyamine Synthetase, GlnA2, Is Involved in Polyamine Catabolism in Streptomyces coelicolor. International journal of molecular sciences. 2022;23(7): 3752.
Krysenko, S., Okoniewski, N., Nentwich, M., Matthews, A., Bauerle, M., Zinser, A., Busche, T., et al. (2022). A Second Gamma-Glutamylpolyamine Synthetase, GlnA2, Is Involved in Polyamine Catabolism in Streptomyces coelicolor. International journal of molecular sciences, 23(7), 3752. https://doi.org/10.3390/ijms23073752
Krysenko, S., Okoniewski, N., Nentwich, M., Matthews, A., Bauerle, M., Zinser, A., Busche, T., Kulik, A., Gursch, S., Kemeny, A., et al. (2022). A Second Gamma-Glutamylpolyamine Synthetase, GlnA2, Is Involved in Polyamine Catabolism in Streptomyces coelicolor. International journal of molecular sciences 23:3752.
Krysenko, S., et al., 2022. A Second Gamma-Glutamylpolyamine Synthetase, GlnA2, Is Involved in Polyamine Catabolism in Streptomyces coelicolor. International journal of molecular sciences, 23(7): 3752.
S. Krysenko, et al., “A Second Gamma-Glutamylpolyamine Synthetase, GlnA2, Is Involved in Polyamine Catabolism in Streptomyces coelicolor”, International journal of molecular sciences, vol. 23, 2022, : 3752.
Krysenko, S., Okoniewski, N., Nentwich, M., Matthews, A., Bauerle, M., Zinser, A., Busche, T., Kulik, A., Gursch, S., Kemeny, A., Bera, A., Wohlleben, W.: A Second Gamma-Glutamylpolyamine Synthetase, GlnA2, Is Involved in Polyamine Catabolism in Streptomyces coelicolor. International journal of molecular sciences. 23, : 3752 (2022).
Krysenko, Sergii, Okoniewski, Nicole, Nentwich, Merle, Matthews, Arne, Bauerle, Moritz, Zinser, Alina, Busche, Tobias, Kulik, Andreas, Gursch, Stephanie, Kemeny, Annika, Bera, Agnieszka, and Wohlleben, Wolfgang. “A Second Gamma-Glutamylpolyamine Synthetase, GlnA2, Is Involved in Polyamine Catabolism in Streptomyces coelicolor”. International journal of molecular sciences 23.7 (2022): 3752.

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 35409114
PubMed | Europe PMC

Suchen in

Google Scholar