Fermentative indole production via bacterial tryptophan synthase alpha subunit and plant indole-3-glycerol phosphate lyase enzymes

Ferrer L, Mindt M, Suarez-Diez M, Jilg T, Zagorščak M, Lee J-H, Gruden K, Wendisch VF, Cankar K (2022)
Journal of Agricultural and Food Chemistry 70(18): 5634−5645.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Ferrer, LennyUniBi; Mindt, MelanieUniBi; Suarez-Diez, Maria; Jilg, Tatjana; Zagorščak, Maja; Lee, Jin-Ho; Gruden, Kristina; Wendisch, Volker F.UniBi ; Cankar, Katarina
Abstract / Bemerkung
Indole is produced in nature by diverse organisms and exhibits a characteristic odor described as animal, fecal, and floral. In addition, it contributes to the flavor in foods, and it is applied in the fragrance and flavor industry. In nature, indole is synthesized either from tryptophan by bacterial tryptophanases (TNAs) or from indole-3-glycerol phosphate (IGP) by plant indole-3-glycerol phosphate lyases (IGLs). While it is widely accepted that the tryptophan synthase α-subunit (TSA) has intrinsically low IGL activity in the absence of the tryptophan synthase β-subunit, in this study, we show that Corynebacterium glutamicum TSA functions as a bona fide IGL and can support fermentative indole production in strains providing IGP. By bioprospecting additional bacterial TSAs and plant IGLs that function as bona fide IGLs were identified. Capturing indole in an overlay enabled indole production to titers of about 0.7 g L-1 in fermentations using C. glutamicum strains expressing either the endogenous TSA gene or the IGL gene from wheat.
Erscheinungsjahr
2022
Zeitschriftentitel
Journal of Agricultural and Food Chemistry
Band
70
Ausgabe
18
Seite(n)
5634−5645
ISSN
0021-8561
eISSN
1520-5118
Page URI
https://pub.uni-bielefeld.de/record/2962458

Zitieren

Ferrer L, Mindt M, Suarez-Diez M, et al. Fermentative indole production via bacterial tryptophan synthase alpha subunit and plant indole-3-glycerol phosphate lyase enzymes. Journal of Agricultural and Food Chemistry. 2022;70(18):5634−5645.
Ferrer, L., Mindt, M., Suarez-Diez, M., Jilg, T., Zagorščak, M., Lee, J. - H., Gruden, K., et al. (2022). Fermentative indole production via bacterial tryptophan synthase alpha subunit and plant indole-3-glycerol phosphate lyase enzymes. Journal of Agricultural and Food Chemistry, 70(18), 5634−5645. https://doi.org/10.1021/acs.jafc.2c01042
Ferrer, L., Mindt, M., Suarez-Diez, M., Jilg, T., Zagorščak, M., Lee, J. - H., Gruden, K., Wendisch, V. F., and Cankar, K. (2022). Fermentative indole production via bacterial tryptophan synthase alpha subunit and plant indole-3-glycerol phosphate lyase enzymes. Journal of Agricultural and Food Chemistry 70, 5634−5645.
Ferrer, L., et al., 2022. Fermentative indole production via bacterial tryptophan synthase alpha subunit and plant indole-3-glycerol phosphate lyase enzymes. Journal of Agricultural and Food Chemistry, 70(18), p 5634−5645.
L. Ferrer, et al., “Fermentative indole production via bacterial tryptophan synthase alpha subunit and plant indole-3-glycerol phosphate lyase enzymes”, Journal of Agricultural and Food Chemistry, vol. 70, 2022, pp. 5634−5645.
Ferrer, L., Mindt, M., Suarez-Diez, M., Jilg, T., Zagorščak, M., Lee, J.-H., Gruden, K., Wendisch, V.F., Cankar, K.: Fermentative indole production via bacterial tryptophan synthase alpha subunit and plant indole-3-glycerol phosphate lyase enzymes. Journal of Agricultural and Food Chemistry. 70, 5634−5645 (2022).
Ferrer, Lenny, Mindt, Melanie, Suarez-Diez, Maria, Jilg, Tatjana, Zagorščak, Maja, Lee, Jin-Ho, Gruden, Kristina, Wendisch, Volker F., and Cankar, Katarina. “Fermentative indole production via bacterial tryptophan synthase alpha subunit and plant indole-3-glycerol phosphate lyase enzymes”. Journal of Agricultural and Food Chemistry 70.18 (2022): 5634−5645.
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2022-05-20T13:36:09Z
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