Metabolic engineering of Corynebacterium glutamicum for sustainable production of the aromatic dicarboxylic acid dipicolinic acid

Schwardmann L, Dransfeld A, Schäffer T, Wendisch VF (2022)
Microorganisms 10(4): 730.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Dipicolinic acid (DPA) is an aromatic dicarboxylic acid that mediates heat-stability and is easily biodegradable and non-toxic. Currently, the production of DPA is fossil-based, but bioproduction of DPA may help to replace fossil-based plastics as it can be used for the production of polyesters or polyamides. Moreover, it serves as a stabilizer for peroxides or organic materials. The antioxidative, antimicrobial and antifungal effects of DPA make it interesting for pharmaceutical applications. In nature, DPA is essential for sporulation of Bacillus and Clostridium species, and its biosynthesis shares the first three reactions with the L-lysine pathway. Corynebacterium glutamicum is a major host for the fermentative production of amino acids, including the million-ton per year production of L-lysine. This study revealed that DPA reduced the growth rate of C. glutamicum to half-maximal at about 1.6 g·L−1. The first de novo production of DPA by C. glutamicum was established by overexpression of dipicolinate synthase genes from Paenibacillus sonchi genomovar riograndensis SBR5 in a C. glutamicum L-lysine producer strain. Upon systems metabolic engineering, DPA production to 2.5 g·L−1 in shake-flask and 1.5 g·L−1 in fed-batch bioreactor cultivations was shown. Moreover, DPA production from the alternative carbon substrates arabinose, xylose, glycerol, and starch was established. Finally, expression of the codon-harmonized phosphite dehydrogenase gene from P. stutzeri enabled phosphite-dependent non-sterile DPA production
Stichworte
dipicolinic acid; Corynebacterium glutamicum; metabolic engineering; sustainable production; nonsterile fermentation; phosphite
Erscheinungsjahr
2022
Zeitschriftentitel
Microorganisms
Band
10
Ausgabe
4
Art.-Nr.
730
eISSN
2076-2607
Finanzierungs-Informationen
Open-Access-Publikationskosten wurden durch die Universität Bielefeld gefördert.
Page URI
https://pub.uni-bielefeld.de/record/2962020

Zitieren

Schwardmann L, Dransfeld A, Schäffer T, Wendisch VF. Metabolic engineering of Corynebacterium glutamicum for sustainable production of the aromatic dicarboxylic acid dipicolinic acid. Microorganisms. 2022;10(4): 730.
Schwardmann, L., Dransfeld, A., Schäffer, T., & Wendisch, V. F. (2022). Metabolic engineering of Corynebacterium glutamicum for sustainable production of the aromatic dicarboxylic acid dipicolinic acid. Microorganisms, 10(4), 730. https://doi.org/10.3390/microorganisms10040730
Schwardmann, Lynn, Dransfeld, Aron, Schäffer, Thomas, and Wendisch, Volker F. 2022. “Metabolic engineering of Corynebacterium glutamicum for sustainable production of the aromatic dicarboxylic acid dipicolinic acid”. Microorganisms 10 (4): 730.
Schwardmann, L., Dransfeld, A., Schäffer, T., and Wendisch, V. F. (2022). Metabolic engineering of Corynebacterium glutamicum for sustainable production of the aromatic dicarboxylic acid dipicolinic acid. Microorganisms 10:730.
Schwardmann, L., et al., 2022. Metabolic engineering of Corynebacterium glutamicum for sustainable production of the aromatic dicarboxylic acid dipicolinic acid. Microorganisms, 10(4): 730.
L. Schwardmann, et al., “Metabolic engineering of Corynebacterium glutamicum for sustainable production of the aromatic dicarboxylic acid dipicolinic acid”, Microorganisms, vol. 10, 2022, : 730.
Schwardmann, L., Dransfeld, A., Schäffer, T., Wendisch, V.F.: Metabolic engineering of Corynebacterium glutamicum for sustainable production of the aromatic dicarboxylic acid dipicolinic acid. Microorganisms. 10, : 730 (2022).
Schwardmann, Lynn, Dransfeld, Aron, Schäffer, Thomas, and Wendisch, Volker F. “Metabolic engineering of Corynebacterium glutamicum for sustainable production of the aromatic dicarboxylic acid dipicolinic acid”. Microorganisms 10.4 (2022): 730.
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2022-03-29T08:27:12Z
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