The Diminishment of Novel Endometrial Carcinoma-Derived Stem-like Cells by Targeting Mitochondrial Bioenergetics and MYC

Helweg L, Windmöller BA, Burghardt L, Storm J, Förster C, Wethkamp N, Wilkens L, Kaltschmidt B, Banz-Jansen C, Kaltschmidt C (2022)
International Journal of Molecular Sciences 23(5): 2426.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Helweg, LaureenUniBi ; Windmöller, Beatrice ArianeUniBi; Burghardt, LeonieUniBi; Storm, Jonathan; Förster, Christine; Wethkamp, Nils; Wilkens, Ludwig; Kaltschmidt, BarbaraUniBi; Banz-Jansen, Constanze; Kaltschmidt, ChristianUniBi
Abstract / Bemerkung
Cancer stem cells (CSCs) are a small subpopulation of tumor cells harboring properties that include self-renewal, multi-lineage differentiation, tumor reconstitution, drug resistance and invasiveness, making them key players in tumor relapse. In the present paper, we develop new CSC models and analyze the molecular pathways involved in survival to identify targets for the establishment of novel therapies. Endometrial carcinoma-derived stem-like cells (ECSCs) were isolated from carcinogenic gynecological tissue and analyzed regarding their expression of prominent CSC markers. Further, they were treated with the MYC-signaling inhibitor KJ-Pyr-9, chemotherapeutic agent carboplatin and type II diabetes medication metformin. ECSC populations express common CSC markers, such as Prominin-1 and CD44 antigen as well as epithelial-to-mesenchymal transition markers, Twist, Snail and Slug, and exhibit the ability to form free-floating spheres. The inhibition of MYC signaling and treatment with carboplatin as well as metformin significantly reduced the cell survival of ECSC-like cells. Further, treatment with metformin significantly decreased the mitochondrial membrane potential of ECSC-like cells, while the extracellular lactate concentration was increased. The established ECSC-like populations represent promising in vitro models to further study the contribution of ECSCs to endometrial carcinogenesis. Targeting MYC signaling as well as mitochondrial bioenergetics has shown promising results in the diminishment of ECSCs, although molecular signaling pathways need further investigations.
Stichworte
cancer stem cells; endometrial cancer; primary endometrial cancer stem cells; MYC; metformin; mitochondria; EMT
Erscheinungsjahr
2022
Zeitschriftentitel
International Journal of Molecular Sciences
Band
23
Ausgabe
5
Art.-Nr.
2426
eISSN
1422-0067
Page URI
https://pub.uni-bielefeld.de/record/2961432

Zitieren

Helweg L, Windmöller BA, Burghardt L, et al. The Diminishment of Novel Endometrial Carcinoma-Derived Stem-like Cells by Targeting Mitochondrial Bioenergetics and MYC. International Journal of Molecular Sciences. 2022;23(5): 2426.
Helweg, L., Windmöller, B. A., Burghardt, L., Storm, J., Förster, C., Wethkamp, N., Wilkens, L., et al. (2022). The Diminishment of Novel Endometrial Carcinoma-Derived Stem-like Cells by Targeting Mitochondrial Bioenergetics and MYC. International Journal of Molecular Sciences, 23(5), 2426. https://doi.org/10.3390/ijms23052426
Helweg, Laureen, Windmöller, Beatrice Ariane, Burghardt, Leonie, Storm, Jonathan, Förster, Christine, Wethkamp, Nils, Wilkens, Ludwig, Kaltschmidt, Barbara, Banz-Jansen, Constanze, and Kaltschmidt, Christian. 2022. “The Diminishment of Novel Endometrial Carcinoma-Derived Stem-like Cells by Targeting Mitochondrial Bioenergetics and MYC”. International Journal of Molecular Sciences 23 (5): 2426.
Helweg, L., Windmöller, B. A., Burghardt, L., Storm, J., Förster, C., Wethkamp, N., Wilkens, L., Kaltschmidt, B., Banz-Jansen, C., and Kaltschmidt, C. (2022). The Diminishment of Novel Endometrial Carcinoma-Derived Stem-like Cells by Targeting Mitochondrial Bioenergetics and MYC. International Journal of Molecular Sciences 23:2426.
Helweg, L., et al., 2022. The Diminishment of Novel Endometrial Carcinoma-Derived Stem-like Cells by Targeting Mitochondrial Bioenergetics and MYC. International Journal of Molecular Sciences, 23(5): 2426.
L. Helweg, et al., “The Diminishment of Novel Endometrial Carcinoma-Derived Stem-like Cells by Targeting Mitochondrial Bioenergetics and MYC”, International Journal of Molecular Sciences, vol. 23, 2022, : 2426.
Helweg, L., Windmöller, B.A., Burghardt, L., Storm, J., Förster, C., Wethkamp, N., Wilkens, L., Kaltschmidt, B., Banz-Jansen, C., Kaltschmidt, C.: The Diminishment of Novel Endometrial Carcinoma-Derived Stem-like Cells by Targeting Mitochondrial Bioenergetics and MYC. International Journal of Molecular Sciences. 23, : 2426 (2022).
Helweg, Laureen, Windmöller, Beatrice Ariane, Burghardt, Leonie, Storm, Jonathan, Förster, Christine, Wethkamp, Nils, Wilkens, Ludwig, Kaltschmidt, Barbara, Banz-Jansen, Constanze, and Kaltschmidt, Christian. “The Diminishment of Novel Endometrial Carcinoma-Derived Stem-like Cells by Targeting Mitochondrial Bioenergetics and MYC”. International Journal of Molecular Sciences 23.5 (2022): 2426.
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2022-02-28T06:22:47Z
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