Fluorescence lifetime DNA-PAINT for multiplexed super-resolution imaging of cells

Oleksiievets N, Sargsyan Y, Thiele JC, Mougios N, Sograte-Idrissi S, Nevskyi O, Gregor I, Opazo F, Thoms S, Enderlein J, Tsukanov R (2022)
Communications Biology 5(1): 38.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Oleksiievets, Nazar; Sargsyan, Yelena; Thiele, Jan Christoph; Mougios, Nikolaos; Sograte-Idrissi, Shama; Nevskyi, Oleksii; Gregor, Ingo; Opazo, Felipe; Thoms, SvenUniBi; Enderlein, Jorg; Tsukanov, Roman
Abstract / Bemerkung
DNA point accumulation for imaging in nanoscale topography (DNA-PAINT) is a powerful super-resolution technique highly suitable for multi-target (multiplexing) bio-imaging. However, multiplexed imaging of cells is still challenging due to the dense and sticky environment inside a cell. Here, we combine fluorescence lifetime imaging microscopy (FLIM) with DNA-PAINT and use the lifetime information as a multiplexing parameter for targets identification. In contrast to Exchange-PAINT, fluorescence lifetime PAINT (FL-PAINT) can image multiple targets simultaneously and does not require any fluid exchange, thus leaving the sample undisturbed and making the use of flow chambers/microfluidic systems unnecessary. We demonstrate the potential of FL-PAINT by simultaneous imaging of up to three targets in a cell using both wide-field FLIM and 3D time-resolved confocal laser scanning microscopy (CLSM). FL-PAINT can be readily combined with other existing techniques of multiplexed imaging and is therefore a perfect candidate for high-throughput multi-target bio-imaging. © 2022. The Author(s).
Erscheinungsjahr
2022
Zeitschriftentitel
Communications Biology
Band
5
Ausgabe
1
Art.-Nr.
38
eISSN
2399-3642
Page URI
https://pub.uni-bielefeld.de/record/2960633

Zitieren

Oleksiievets N, Sargsyan Y, Thiele JC, et al. Fluorescence lifetime DNA-PAINT for multiplexed super-resolution imaging of cells. Communications Biology . 2022;5(1): 38.
Oleksiievets, N., Sargsyan, Y., Thiele, J. C., Mougios, N., Sograte-Idrissi, S., Nevskyi, O., Gregor, I., et al. (2022). Fluorescence lifetime DNA-PAINT for multiplexed super-resolution imaging of cells. Communications Biology , 5(1), 38. https://doi.org/10.1038/s42003-021-02976-4
Oleksiievets, N., Sargsyan, Y., Thiele, J. C., Mougios, N., Sograte-Idrissi, S., Nevskyi, O., Gregor, I., Opazo, F., Thoms, S., Enderlein, J., et al. (2022). Fluorescence lifetime DNA-PAINT for multiplexed super-resolution imaging of cells. Communications Biology 5:38.
Oleksiievets, N., et al., 2022. Fluorescence lifetime DNA-PAINT for multiplexed super-resolution imaging of cells. Communications Biology , 5(1): 38.
N. Oleksiievets, et al., “Fluorescence lifetime DNA-PAINT for multiplexed super-resolution imaging of cells”, Communications Biology , vol. 5, 2022, : 38.
Oleksiievets, N., Sargsyan, Y., Thiele, J.C., Mougios, N., Sograte-Idrissi, S., Nevskyi, O., Gregor, I., Opazo, F., Thoms, S., Enderlein, J., Tsukanov, R.: Fluorescence lifetime DNA-PAINT for multiplexed super-resolution imaging of cells. Communications Biology . 5, : 38 (2022).
Oleksiievets, Nazar, Sargsyan, Yelena, Thiele, Jan Christoph, Mougios, Nikolaos, Sograte-Idrissi, Shama, Nevskyi, Oleksii, Gregor, Ingo, Opazo, Felipe, Thoms, Sven, Enderlein, Jorg, and Tsukanov, Roman. “Fluorescence lifetime DNA-PAINT for multiplexed super-resolution imaging of cells”. Communications Biology 5.1 (2022): 38.

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