Characterization of the Leucocyte Immunoglobulin-like Receptor B4 (Lilrb4) Expression in Microglia

Kretzschmar F, Piecha R, Jahn J, Potru P, Spittau B (2021)
Biology 10(12): 1300.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Kretzschmar, Felix; Piecha, Robin; Jahn, Jannik; Potru, PhaniUniBi; Spittau, BjörnUniBi
Abstract / Bemerkung
As resident innate immune cells of the CNS, microglia play important essential roles during physiological and pathological situations. Recent reports have described the expression of Lilrb4 in disease-associated and aged microglia. Here, we characterized the expression of Lilrb4 in microglia in vitro and in vivo in comparison with bone marrow-derived monocytes and peritoneal macrophages in mice. Using BV2 cells, primary microglia cultures as well as ex vivo isolated microglia and myeloid cells in combination with qPCR and flow cytometry, we were able to provide a comprehensive characterization of Lilrb4 expression in distinct mouse myeloid cells. Whereas microglia in vivo display low expression of Lilrb4, primary microglia cultures present high levels of surface LILRB4. Among the analyzed peripheral myeloid cells, peritoneal macrophages showed the highest expression levels of Lilrb4. Moreover, LPS treatment and inhibition of microglial TGFβ signaling resulted in significant increases of LILRB4 cell surface levels. Taken together, our data indicate that LILRB4 is a reliable surface marker for activated microglia and further demonstrate that microglial TGFβ signaling is involved in the regulation of Lilrb4 expression during LPS-induced microglia activation.
Stichworte
Lilrb4; microglia; TGFβ1; LPS
Erscheinungsjahr
2021
Zeitschriftentitel
Biology
Band
10
Ausgabe
12
Art.-Nr.
1300
eISSN
2079-7737
Finanzierungs-Informationen
Open-Access-Publikationskosten wurden durch die Universität Bielefeld gefördert.
Page URI
https://pub.uni-bielefeld.de/record/2959825

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Kretzschmar F, Piecha R, Jahn J, Potru P, Spittau B. Characterization of the Leucocyte Immunoglobulin-like Receptor B4 (Lilrb4) Expression in Microglia. Biology. 2021;10(12): 1300.
Kretzschmar, F., Piecha, R., Jahn, J., Potru, P., & Spittau, B. (2021). Characterization of the Leucocyte Immunoglobulin-like Receptor B4 (Lilrb4) Expression in Microglia. Biology, 10(12), 1300. https://doi.org/10.3390/biology10121300
Kretzschmar, Felix, Piecha, Robin, Jahn, Jannik, Potru, Phani, and Spittau, Björn. 2021. “Characterization of the Leucocyte Immunoglobulin-like Receptor B4 (Lilrb4) Expression in Microglia”. Biology 10 (12): 1300.
Kretzschmar, F., Piecha, R., Jahn, J., Potru, P., and Spittau, B. (2021). Characterization of the Leucocyte Immunoglobulin-like Receptor B4 (Lilrb4) Expression in Microglia. Biology 10:1300.
Kretzschmar, F., et al., 2021. Characterization of the Leucocyte Immunoglobulin-like Receptor B4 (Lilrb4) Expression in Microglia. Biology, 10(12): 1300.
F. Kretzschmar, et al., “Characterization of the Leucocyte Immunoglobulin-like Receptor B4 (Lilrb4) Expression in Microglia”, Biology, vol. 10, 2021, : 1300.
Kretzschmar, F., Piecha, R., Jahn, J., Potru, P., Spittau, B.: Characterization of the Leucocyte Immunoglobulin-like Receptor B4 (Lilrb4) Expression in Microglia. Biology. 10, : 1300 (2021).
Kretzschmar, Felix, Piecha, Robin, Jahn, Jannik, Potru, Phani, and Spittau, Björn. “Characterization of the Leucocyte Immunoglobulin-like Receptor B4 (Lilrb4) Expression in Microglia”. Biology 10.12 (2021): 1300.
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2021-12-09T07:26:46Z
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