Dual color DMD-SIM by temperature-controlled laser wavelength matching

Lachetta M, Wiebusch G, Hübner W, Schulte am Esch J, Huser T, Müller M (2021)
Optics Express 29(24): 39696-39708.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Structured illumination microscopy (SIM) is a fast and gentle super-resolution fluorescence imaging technique, featuring live-cell compatible excitation light levels and high imaging speeds. To achieve SIM, spatial modulation of the fluorescence excitation light is employed. This is typically achieved by interfering coherent laser beams in the sample plane, which are often created by spatial light modulators (SLMs). Digital micromirror devices (DMDs) are a form of SLMs with certain advantages, such as high speed, low cost and wide availability, which present certain hurdles in their implementation, mainly the blazed grating effect caused by the jagged surface structure of the tilted mirrors. Recent works have studied this effect through modelling, simulations and experiments, and laid out possible implementations of multi-color SIM imaging based on DMDs. Here, we present an implementation of a dual-color DMD based SIM microscope using temperature-controlled wavelength matching. By carefully controlling the output wavelength of a diode laser by temperature, we can tune two laser wavelengths in such a way that no opto-mechanical realignment of the SIM setup is necessary when switching between both wavelengths. This reduces system complexity and increases imaging speed. With measurements on nano-bead reference samples, as well as the actin skeleton and membrane of fixed U2OS cells, we demonstrate the capabilities of the setup. (C) 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
Erscheinungsjahr
2021
Zeitschriftentitel
Optics Express
Band
29
Ausgabe
24
Seite(n)
39696-39708
eISSN
1094-4087
Finanzierungs-Informationen
Open-Access-Publikationskosten wurden durch die Universität Bielefeld gefördert.
Page URI
https://pub.uni-bielefeld.de/record/2959751

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Lachetta M, Wiebusch G, Hübner W, Schulte am Esch J, Huser T, Müller M. Dual color DMD-SIM by temperature-controlled laser wavelength matching. Optics Express. 2021;29(24):39696-39708.
Lachetta, M., Wiebusch, G., Hübner, W., Schulte am Esch, J., Huser, T., & Müller, M. (2021). Dual color DMD-SIM by temperature-controlled laser wavelength matching. Optics Express, 29(24), 39696-39708. https://doi.org/10.1364/OE.437822
Lachetta, Mario, Wiebusch, Gerd, Hübner, Wolfgang, Schulte am Esch, Jan, Huser, Thomas, and Müller, Marcel. 2021. “Dual color DMD-SIM by temperature-controlled laser wavelength matching”. Optics Express 29 (24): 39696-39708.
Lachetta, M., Wiebusch, G., Hübner, W., Schulte am Esch, J., Huser, T., and Müller, M. (2021). Dual color DMD-SIM by temperature-controlled laser wavelength matching. Optics Express 29, 39696-39708.
Lachetta, M., et al., 2021. Dual color DMD-SIM by temperature-controlled laser wavelength matching. Optics Express, 29(24), p 39696-39708.
M. Lachetta, et al., “Dual color DMD-SIM by temperature-controlled laser wavelength matching”, Optics Express, vol. 29, 2021, pp. 39696-39708.
Lachetta, M., Wiebusch, G., Hübner, W., Schulte am Esch, J., Huser, T., Müller, M.: Dual color DMD-SIM by temperature-controlled laser wavelength matching. Optics Express. 29, 39696-39708 (2021).
Lachetta, Mario, Wiebusch, Gerd, Hübner, Wolfgang, Schulte am Esch, Jan, Huser, Thomas, and Müller, Marcel. “Dual color DMD-SIM by temperature-controlled laser wavelength matching”. Optics Express 29.24 (2021): 39696-39708.
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2022-01-21T08:29:34Z
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