A synthetic biology approach to study carotenoid production in Corynebacterium glutamicum: read-out by a genetically encoded biosensor combined with perturbing native gene expression by CRISPRi

Henke, Nadja Alina; Göttl, Vanessa; Schmitt, Ina; Peters-Wendisch, Petra; Wendisch, Volker F.

A synthetic biology approach to study carotenoid production in Corynebacterium glutamicum: read-out by a genetically encoded biosensor combined with perturbing native gene expression by CRISPRi

Henke NA, Göttl V, Schmitt I, Peters-Wendisch P, Wendisch VF (2022)
In: Carotenoids: Carotenoid and apocarotenoid biosynthesis, metabolic engineering and synthetic biology. Wurtzel E (Ed); Methods in Enzymology, 671. Elsevier : 383-419.

Sammelwerksbeitrag | Veröffentlicht | Englisch

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DOI

https://doi.org/10.1016/bs.mie.2021.11.026

Autor*in

Henke, Nadja Alina^UniBi; Göttl, Vanessa^UniBi ; Schmitt, Ina^UniBi ; Peters-Wendisch, Petra^UniBi; Wendisch, Volker F.^UniBi

Herausgeber*in

Wurtzel, Eleanore

Einrichtung

Fakultät für Biologie > Genetik der Prokaryoten
Centrum für Biotechnologie > Arbeitsgruppe V. Wendisch

Abstract / Bemerkung

Carotenoids: Carotenoid and Apocarotenoid Biosynthesis, Metabolic Engineering and Synthetic Biology, Volume 671, the latest release in the Methods of Enzymology series highlights new advances in the field with chapters on Metabolomics-based analysis of carotenoids and related metabolites in various species via quantitative trait loci and genome wide association mapping approaches, Using bacteria for functional analysis of genes encoding carotenoid biosynthetic enzymes, Rice Callus as a High Throughput Platform for Synthetic Biology and Metabolic Engineering of Carotenoids, Transient expression in Nicotiana benthamiana: A simple platform to investigate genes encoding carotenoid biosynthesis enzymes from diverse algal lineages, and much more. Additional chapters in this new release cover Protein-protein interaction techniques to investigate post-translational regulation of carotenogenesis, The isolation of sub-chromoplast structures from tomato and capsicum fruit, Carrot protoplasts as a suitable method for protein subcellular localization, High throughput production and characterization of carotenoid enzymes for structural and functional studies, Production and structural characterization of the cytochrome P450 enzymes in carotene ring hydroxylation, and more.

Erscheinungsjahr

2022

Buchtitel

Carotenoids: Carotenoid and apocarotenoid biosynthesis, metabolic engineering and synthetic biology

Serientitel

Methods in Enzymology

Band

671

Seite(n)

383-419

ISBN

9780323913539

Page URI

https://pub.uni-bielefeld.de/record/2959499

Zitieren

Henke NA, Göttl V, Schmitt I, Peters-Wendisch P, Wendisch VF. A synthetic biology approach to study carotenoid production in Corynebacterium glutamicum: read-out by a genetically encoded biosensor combined with perturbing native gene expression by CRISPRi. In: Wurtzel E, ed. Carotenoids: Carotenoid and apocarotenoid biosynthesis, metabolic engineering and synthetic biology. Methods in Enzymology. Vol 671. Elsevier ; 2022: 383-419.

Henke, N. A., Göttl, V., Schmitt, I., Peters-Wendisch, P., & Wendisch, V. F. (2022). A synthetic biology approach to study carotenoid production in Corynebacterium glutamicum: read-out by a genetically encoded biosensor combined with perturbing native gene expression by CRISPRi. In E. Wurtzel (Ed.), Methods in Enzymology: Vol. 671. Carotenoids: Carotenoid and apocarotenoid biosynthesis, metabolic engineering and synthetic biology (pp. 383-419). Elsevier . https://doi.org/10.1016/bs.mie.2021.11.026

Henke, Nadja Alina, Göttl, Vanessa, Schmitt, Ina, Peters-Wendisch, Petra, and Wendisch, Volker F. 2022. “A synthetic biology approach to study carotenoid production in Corynebacterium glutamicum: read-out by a genetically encoded biosensor combined with perturbing native gene expression by CRISPRi”. In Carotenoids: Carotenoid and apocarotenoid biosynthesis, metabolic engineering and synthetic biology, ed. Eleanore Wurtzel, 671:383-419. Methods in Enzymology. Elsevier .

Henke, N. A., Göttl, V., Schmitt, I., Peters-Wendisch, P., and Wendisch, V. F. (2022). “A synthetic biology approach to study carotenoid production in Corynebacterium glutamicum: read-out by a genetically encoded biosensor combined with perturbing native gene expression by CRISPRi” in Carotenoids: Carotenoid and apocarotenoid biosynthesis, metabolic engineering and synthetic biology, Wurtzel, E. ed. Methods in Enzymology, vol. 671, (Elsevier ), 383-419.

Henke, N.A., et al., 2022. A synthetic biology approach to study carotenoid production in Corynebacterium glutamicum: read-out by a genetically encoded biosensor combined with perturbing native gene expression by CRISPRi. In E. Wurtzel, ed. Carotenoids: Carotenoid and apocarotenoid biosynthesis, metabolic engineering and synthetic biology. Methods in Enzymology. no.671 Elsevier , pp. 383-419.

N.A. Henke, et al., “A synthetic biology approach to study carotenoid production in Corynebacterium glutamicum: read-out by a genetically encoded biosensor combined with perturbing native gene expression by CRISPRi”, Carotenoids: Carotenoid and apocarotenoid biosynthesis, metabolic engineering and synthetic biology, E. Wurtzel, ed., Methods in Enzymology, vol. 671, Elsevier , 2022, pp.383-419.

Henke, N.A., Göttl, V., Schmitt, I., Peters-Wendisch, P., Wendisch, V.F.: A synthetic biology approach to study carotenoid production in Corynebacterium glutamicum: read-out by a genetically encoded biosensor combined with perturbing native gene expression by CRISPRi. In: Wurtzel, E. (ed.) Carotenoids: Carotenoid and apocarotenoid biosynthesis, metabolic engineering and synthetic biology. Methods in Enzymology. 671, p. 383-419. Elsevier (2022).

Henke, Nadja Alina, Göttl, Vanessa, Schmitt, Ina, Peters-Wendisch, Petra, and Wendisch, Volker F. “A synthetic biology approach to study carotenoid production in Corynebacterium glutamicum: read-out by a genetically encoded biosensor combined with perturbing native gene expression by CRISPRi”. Carotenoids: Carotenoid and apocarotenoid biosynthesis, metabolic engineering and synthetic biology. Ed. Eleanore Wurtzel. Elsevier , 2022.Vol. 671. Methods in Enzymology. 383-419.

Daten bereitgestellt von European Bioinformatics Institute (EBI)