Protein Nanopore Membranes Prepared by a Simple Langmuir-Schaefer Approach

Schwieters MS, Mathieu-Gaedke M, Westphal M, Dalpke R, Dirksen M, Qi D, Grull M, Bick T, TaSSler S, Sauer DF, Bonn M, et al. (2021)
Small 17(46): 2102975.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Schwieters, Magnus S; Mathieu-Gaedke, Maria; Westphal, MichaelUniBi; Dalpke, RaphaelUniBi; Dirksen, MaximUniBi; Qi, Daizong; Grull, Marco; Bick, Thomas; TaSSler, Stephanie; Sauer, Daniel F; Bonn, Mischa; Wendler, Petra
Alle
Abstract / Bemerkung
Filtration through membranes with nanopores is typically associated with high transmembrane pressures and high energy consumption. This problem can be addressed by reducing the respective membrane thickness. Here, a simple procedure is described to prepare ultrathin membranes based on protein nanopores, which exhibit excellent water permeance, two orders of magnitude superior to comparable, industrially applied membranes. Furthermore, incorporation of either closed or open protein nanopores allows tailoring the membrane's ion permeability. To form such membranes, the transmembrane protein ferric hydroxamate uptake protein component A (FhuA) or its open-pore variant are assembled at the air-water interface of a Langmuir trough, compressed to a dense film, crosslinked by glutaraldehyde, and transferred to various support materials. This approach allows to prepare monolayer or multilayer membranes with a very high density of protein nanopores. Freestanding membranes covering holes up to 5 mum in diameter are visualized by atomic force microscopy (AFM), helium ion microscopy, and transmission electron microscopy. AFM PeakForce quantitative nanomechanical property mapping (PeakForce QNM) demonstrates remarkable mechanical stability and elastic properties of freestanding monolayer membranes with a thickness of only 5nm. The new protein membrane can pave the way to energy-efficient nanofiltration. © 2021 The Authors. Small published by Wiley-VCH GmbH.
Erscheinungsjahr
2021
Zeitschriftentitel
Small
Band
17
Ausgabe
46
Art.-Nr.
2102975
eISSN
1613-6829
Page URI
https://pub.uni-bielefeld.de/record/2958350

Zitieren

Schwieters MS, Mathieu-Gaedke M, Westphal M, et al. Protein Nanopore Membranes Prepared by a Simple Langmuir-Schaefer Approach. Small. 2021;17(46): 2102975.
Schwieters, M. S., Mathieu-Gaedke, M., Westphal, M., Dalpke, R., Dirksen, M., Qi, D., Grull, M., et al. (2021). Protein Nanopore Membranes Prepared by a Simple Langmuir-Schaefer Approach. Small, 17(46), 2102975. https://doi.org/10.1002/smll.202102975
Schwieters, Magnus S, Mathieu-Gaedke, Maria, Westphal, Michael, Dalpke, Raphael, Dirksen, Maxim, Qi, Daizong, Grull, Marco, et al. 2021. “Protein Nanopore Membranes Prepared by a Simple Langmuir-Schaefer Approach”. Small 17 (46): 2102975.
Schwieters, M. S., Mathieu-Gaedke, M., Westphal, M., Dalpke, R., Dirksen, M., Qi, D., Grull, M., Bick, T., TaSSler, S., Sauer, D. F., et al. (2021). Protein Nanopore Membranes Prepared by a Simple Langmuir-Schaefer Approach. Small 17:2102975.
Schwieters, M.S., et al., 2021. Protein Nanopore Membranes Prepared by a Simple Langmuir-Schaefer Approach. Small, 17(46): 2102975.
M.S. Schwieters, et al., “Protein Nanopore Membranes Prepared by a Simple Langmuir-Schaefer Approach”, Small, vol. 17, 2021, : 2102975.
Schwieters, M.S., Mathieu-Gaedke, M., Westphal, M., Dalpke, R., Dirksen, M., Qi, D., Grull, M., Bick, T., TaSSler, S., Sauer, D.F., Bonn, M., Wendler, P., Hellweg, T., Beyer, A., Gölzhäuser, A., Schwaneberg, U., Glebe, U., Boker, A.: Protein Nanopore Membranes Prepared by a Simple Langmuir-Schaefer Approach. Small. 17, : 2102975 (2021).
Schwieters, Magnus S, Mathieu-Gaedke, Maria, Westphal, Michael, Dalpke, Raphael, Dirksen, Maxim, Qi, Daizong, Grull, Marco, Bick, Thomas, TaSSler, Stephanie, Sauer, Daniel F, Bonn, Mischa, Wendler, Petra, Hellweg, Thomas, Beyer, André, Gölzhäuser, Armin, Schwaneberg, Ulrich, Glebe, Ulrich, and Boker, Alexander. “Protein Nanopore Membranes Prepared by a Simple Langmuir-Schaefer Approach”. Small 17.46 (2021): 2102975.

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