Head-to-head evaluation of seven different seroassays including direct viral neutralisation in a representative cohort for SARS-CoV-2

Olbrich L, Castelletti N, Schälte Y, Garí M, Pütz P, Bakuli A, Pritsch M, Kroidl I, Saathoff E, Guggenbuehl Noller J, Fingerle V, et al. (2021)
Journal of General Virology 102(10).

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Olbrich, L; Castelletti, N; Schälte, Y; Garí, M; Pütz, PeterUniBi; Bakuli, A; Pritsch, M ; Kroidl, I; Saathoff, E; Guggenbuehl Noller, J; Fingerle, V; Le Gleut, R
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Abstract / Bemerkung
A number of seroassays are available for SARS-CoV-2 testing; yet, head-to-head evaluations of different testing principles are limited, especially using raw values rather than categorical data. In addition, identifying correlates of protection is of utmost importance, and comparisons of available testing systems with functional assays, such as direct viral neutralisation, are needed.We analysed 6658 samples consisting of true-positives (n=193), true-negatives (n=1091), and specimens of unknown status (n=5374). For primary testing, we used Euroimmun-Anti-SARS-CoV-2-ELISA-IgA/IgG and Roche-Elecsys-Anti-SARS-CoV-2. Subsequently virus-neutralisation, GeneScriptcPass, VIRAMED-SARS-CoV-2-ViraChip, and Mikrogen-recomLine-SARS-CoV-2-IgG were applied for confirmatory testing. Statistical modelling generated optimised assay cut-off thresholds. Sensitivity of Euroimmun-anti-S1-IgA was 64.8%, specificity 93.3% (manufacturer's cut-off); for Euroimmun-anti-S1-IgG, sensitivity was 77.2/79.8% (manufacturer's/optimised cut-offs), specificity 98.0/97.8%; Roche-anti-N sensitivity was 85.5/88.6%, specificity 99.8/99.7%. In true-positives, mean and median Euroimmun-anti-S1-IgA and -IgG titres decreased 30/90 days after RT-PCR-positivity, Roche-anti-N titres decreased significantly later. Virus-neutralisation was 80.6% sensitive, 100.0% specific (≥1:5 dilution). Neutralisation surrogate tests (GeneScriptcPass, Mikrogen-recomLine-RBD) were >94.9% sensitive and >98.1% specific. Optimised cut-offs improved test performances of several tests. Confirmatory testing with virus-neutralisation might be complemented with GeneScriptcPassTM or recomLine-RBD for certain applications. Head-to-head comparisons given here aim to contribute to the refinement of testing strategies for individual and public health use.
Erscheinungsjahr
2021
Zeitschriftentitel
Journal of General Virology
Band
102
Ausgabe
10
eISSN
1465-2099
Page URI
https://pub.uni-bielefeld.de/record/2957769

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Olbrich L, Castelletti N, Schälte Y, et al. Head-to-head evaluation of seven different seroassays including direct viral neutralisation in a representative cohort for SARS-CoV-2. Journal of General Virology. 2021;102(10).
Olbrich, L., Castelletti, N., Schälte, Y., Garí, M., Pütz, P., Bakuli, A., Pritsch, M., et al. (2021). Head-to-head evaluation of seven different seroassays including direct viral neutralisation in a representative cohort for SARS-CoV-2. Journal of General Virology, 102(10). https://doi.org/10.1099/jgv.0.001653
Olbrich, L., Castelletti, N., Schälte, Y., Garí, M., Pütz, P., Bakuli, A., Pritsch, M., Kroidl, I., Saathoff, E., Guggenbuehl Noller, J., et al. (2021). Head-to-head evaluation of seven different seroassays including direct viral neutralisation in a representative cohort for SARS-CoV-2. Journal of General Virology 102.
Olbrich, L., et al., 2021. Head-to-head evaluation of seven different seroassays including direct viral neutralisation in a representative cohort for SARS-CoV-2. Journal of General Virology, 102(10).
L. Olbrich, et al., “Head-to-head evaluation of seven different seroassays including direct viral neutralisation in a representative cohort for SARS-CoV-2”, Journal of General Virology, vol. 102, 2021.
Olbrich, L., Castelletti, N., Schälte, Y., Garí, M., Pütz, P., Bakuli, A., Pritsch, M., Kroidl, I., Saathoff, E., Guggenbuehl Noller, J., Fingerle, V., Le Gleut, R., Gilberg, L., Brand, I., Falk, P., Markgraf, A., Deák, F., Riess, F., Diefenbach, M., Eser, T., Weinauer, F., Martin, S., Quenzel, E.-M., Becker, M., Durner, J., Girl, P., Müller, K., Radon, K., Fuchs, C., Wölfel, R., Hasenauer, J., Hoelscher, M., Wieser, A.: Head-to-head evaluation of seven different seroassays including direct viral neutralisation in a representative cohort for SARS-CoV-2. Journal of General Virology. 102, (2021).
Olbrich, L, Castelletti, N, Schälte, Y, Garí, M, Pütz, Peter, Bakuli, A, Pritsch, M, Kroidl, I, Saathoff, E, Guggenbuehl Noller, J, Fingerle, V, Le Gleut, R, Gilberg, L, Brand, I, Falk, P, Markgraf, A, Deák, F, Riess, F, Diefenbach, M, Eser, T, Weinauer, F, Martin, S, Quenzel, E.-M., Becker, M, Durner, J, Girl, P, Müller, K, Radon, K, Fuchs, Christiane, Wölfel, R, Hasenauer, J, Hoelscher, M, and Wieser, A. “Head-to-head evaluation of seven different seroassays including direct viral neutralisation in a representative cohort for SARS-CoV-2”. Journal of General Virology 102.10 (2021).

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