Stable DOPG/Glycyrrhizin Vesicles with a Wide Range of Mixing Ratios: Structure and Stability as Seen by Scattering Experiments and Cryo-TEM

Dargel, Carina; Gräbitz-Bräuer, Friederike; Geisler, Ramsia; Fandrich, Pascal; Hannappel, Yvonne; Porcar, Lionel; Hellweg, Thomas

Stable DOPG/Glycyrrhizin Vesicles with a Wide Range of Mixing Ratios: Structure and Stability as Seen by Scattering Experiments and Cryo-TEM

Dargel C, Gräbitz-Bräuer F, Geisler R, Fandrich P, Hannappel Y, Porcar L, Hellweg T (2021)
Molecules 26(16): 4959.

Zeitschriftenaufsatz | Veröffentlicht | Englisch

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DOI

https://doi.org/10.3390/molecules26164959

URN

urn:nbn:de:0070-pub-29567796

Autor*in

Dargel, Carina^UniBi; Gräbitz-Bräuer, Friederike^UniBi; Geisler, Ramsia; Fandrich, Pascal^UniBi; Hannappel, Yvonne^UniBi; Porcar, Lionel; Hellweg, Thomas^UniBi

Einrichtung

Fakultät für Chemie > Physikalische und Biophysikalische Chemie

Abstract / Bemerkung

Phosphatidylglycerols represent a large share of the lipids in the plasmamembrane of procaryotes. Therefore, this study investigates the role of charged lipids in the plasma membrane with respect to the interaction of the antiviral saponin glycyrrhizin with such membranes. Glycyrrhizin is a natural triterpenic-based surfactant found in licorice. Vesicles made of 1,2-dioleoyl-sn-glycero-3-phospho-rac-(1’-glycerol) (DOPG)/glycyrrhizin are characterized by small-angle scattering with neutrons and X-rays (SANS and SAXS). Small-angle scattering data are first evaluated by the model-independent modified Kratky–Porod method and afterwards fitted by a model describing the shape of small unilamellar vesicles (SUV) with an internal head-tail contrast. Complete miscibility of DOPG and glycyrrhizin was revealed even at a ratio of lipid:saponin of 1:1. Additional information about the chain-chain correlation distance of the lipid/saponin mixtures in the SUV structures is obtained from wide-angle X-ray scattering (WAXS).

Stichworte

DOPG; glycyrrhizin; small unilamellar vesicle (SUV); SAXS and SANS; WAX

Erscheinungsjahr

2021

Zeitschriftentitel

Molecules

Band

Ausgabe

Art.-Nr.

4959

Urheberrecht / Lizenzen

Creative Commons Namensnennung 4.0 International Public License (CC-BY 4.0)

eISSN

1420-3049

Finanzierungs-Informationen

Open-Access-Publikationskosten wurden durch die Universität Bielefeld gefördert.

Page URI

https://pub.uni-bielefeld.de/record/2956779

Zitieren

Dargel C, Gräbitz-Bräuer F, Geisler R, et al. Stable DOPG/Glycyrrhizin Vesicles with a Wide Range of Mixing Ratios: Structure and Stability as Seen by Scattering Experiments and Cryo-TEM. Molecules. 2021;26(16): 4959.

Dargel, C., Gräbitz-Bräuer, F., Geisler, R., Fandrich, P., Hannappel, Y., Porcar, L., & Hellweg, T. (2021). Stable DOPG/Glycyrrhizin Vesicles with a Wide Range of Mixing Ratios: Structure and Stability as Seen by Scattering Experiments and Cryo-TEM. Molecules, 26(16), 4959. https://doi.org/10.3390/molecules26164959

Dargel, Carina, Gräbitz-Bräuer, Friederike, Geisler, Ramsia, Fandrich, Pascal, Hannappel, Yvonne, Porcar, Lionel, and Hellweg, Thomas. 2021. “Stable DOPG/Glycyrrhizin Vesicles with a Wide Range of Mixing Ratios: Structure and Stability as Seen by Scattering Experiments and Cryo-TEM”. Molecules 26 (16): 4959.

Dargel, C., Gräbitz-Bräuer, F., Geisler, R., Fandrich, P., Hannappel, Y., Porcar, L., and Hellweg, T. (2021). Stable DOPG/Glycyrrhizin Vesicles with a Wide Range of Mixing Ratios: Structure and Stability as Seen by Scattering Experiments and Cryo-TEM. Molecules 26:4959.

Dargel, C., et al., 2021. Stable DOPG/Glycyrrhizin Vesicles with a Wide Range of Mixing Ratios: Structure and Stability as Seen by Scattering Experiments and Cryo-TEM. Molecules, 26(16): 4959.

C. Dargel, et al., “Stable DOPG/Glycyrrhizin Vesicles with a Wide Range of Mixing Ratios: Structure and Stability as Seen by Scattering Experiments and Cryo-TEM”, Molecules, vol. 26, 2021, : 4959.

Dargel, C., Gräbitz-Bräuer, F., Geisler, R., Fandrich, P., Hannappel, Y., Porcar, L., Hellweg, T.: Stable DOPG/Glycyrrhizin Vesicles with a Wide Range of Mixing Ratios: Structure and Stability as Seen by Scattering Experiments and Cryo-TEM. Molecules. 26, : 4959 (2021).

Dargel, Carina, Gräbitz-Bräuer, Friederike, Geisler, Ramsia, Fandrich, Pascal, Hannappel, Yvonne, Porcar, Lionel, and Hellweg, Thomas. “Stable DOPG/Glycyrrhizin Vesicles with a Wide Range of Mixing Ratios: Structure and Stability as Seen by Scattering Experiments and Cryo-TEM”. Molecules 26.16 (2021): 4959.

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