PUB - Publikationen an der Universität Bielefeld

Strain M2^Twas isolated from the beach of Cuxhaven, Wadden Sea, Germany, in course of a program to attain new producers of bioactive natural products. Strain M2^Tproduces litoralimycin and sulfomycin-type thiopeptides. Bioinformatic analysis revealed a potential biosynthetic gene cluster encoding for the M2^Tthiopeptides. The strain is Gram-stain-positive, rod shaped, non-motile, spore forming, showing a yellow colony color and forms extensively branched substrate mycelium and aerial hyphae. Inferred from the 16S rRNA gene phylogeny strain M2^Taffiliates with the genusStreptomonospora.It shows 96.6% 16S rRNA gene sequence similarity to the type speciesStreptomonospora salinaDSM 44593 ^Tand forms a distinct branch withStreptomonospora sediminisDSM 45723 ^Twith 97.0% 16S rRNA gene sequence similarity. Genome-based phylogenetic analysis revealed that M2^Tis closely related toStreptomonospora albaYIM 90003 ^Twith a digital DNA-DNA hybridisation (dDDH) value of 26.6%. The predominant menaquinones of M2^Tare MK-10(H₆), MK-10(H₈), and MK-11(H₆) (> 10%). Major cellular fatty acids are iso-C_16:0,anteiso C_17:0and C_18:010-methyl. The polar lipid profile consisted of diphosphatidylglycerol phosphatidyl glycerol, phosphatidylinositol, phosphatidylcholine, phosphatidylethanolamine, three glycolipids, two unknown phospholipids, and two unknown lipids. The genome size of type strain M2^Tis 5,878,427 bp with 72.1 mol % G + C content. Based on the results obtained from phylogenetic and chemotaxonomic studies, strain M2^T(= DSM 106425 ^T = NCCB 100650 ^T) is considered to represent a novel species within the genusStreptomonosporafor which the nameStreptomonospora litoralissp. nov. is proposed.