Functional characterisation of banana (Musa spp.) 2-oxoglutarate- dependent dioxygenases involved in flavonoid biosynthesis

Busche M, Acatay C, Martens S, Weisshaar B, Stracke R (2021)
Frontiers in Plant Science 12: 701780.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Busche, MareikeUniBi ; Acatay, Christopher; Martens, Stefan; Weisshaar, BerndUniBi ; Stracke, RalfUniBi
Abstract / Bemerkung
Bananas (Musa) are non-grass, monocotyledonous, perennial plants that are well-known for their edible fruits. Their cultivation provides food security and employment opportunities in many countries. Banana fruits contain high levels of minerals and phytochemicals, including flavonoids, which are beneficial for human nutrition. To broaden the knowledge on flavonoid biosynthesis in this major crop plant, we aimed to identify and functionally characterise selected structural genes encoding 2-oxoglutarate‐dependent dioxygenases, involved in the formation of the flavonoid aglycon. Musa candidates genes predicted to encode flavanone 3 hydroxylase (F3H), flavonol synthase (FLS) and anthocyanidin synthase (ANS) were assayed. Enzymatic functionalities of the recombinant proteins were confirmed in vivo using bioconversion assays. Moreover, transgenic analyses in corresponding Arabidopsis thaliana mutants showed that MusaF3H, MusaFLS and MusaANS were able to complement the respective loss-of-function phenotypes, thus verifying functionality of the enzymes in planta. Knowledge gained from this work provides a new aspect for further research towards genetic engineering of flavonoid biosynthesis in banana fruits to increase their antioxidant activity and nutritional value.
Stichworte
banana; specialised metabolites; flavanone 3-hydroxylases; flavonol synthase; anthocyanidin synthase
Erscheinungsjahr
2021
Zeitschriftentitel
Frontiers in Plant Science
Band
12
Art.-Nr.
701780
eISSN
1664-462X
Finanzierungs-Informationen
Open-Access-Publikationskosten wurden durch die Universität Bielefeld gefördert.
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https://pub.uni-bielefeld.de/record/2956345

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Busche M, Acatay C, Martens S, Weisshaar B, Stracke R. Functional characterisation of banana (Musa spp.) 2-oxoglutarate- dependent dioxygenases involved in flavonoid biosynthesis. Frontiers in Plant Science. 2021;12: 701780.
Busche, M., Acatay, C., Martens, S., Weisshaar, B., & Stracke, R. (2021). Functional characterisation of banana (Musa spp.) 2-oxoglutarate- dependent dioxygenases involved in flavonoid biosynthesis. Frontiers in Plant Science, 12, 701780. https://doi.org/10.3389/fpls.2021.701780
Busche, M., Acatay, C., Martens, S., Weisshaar, B., and Stracke, R. (2021). Functional characterisation of banana (Musa spp.) 2-oxoglutarate- dependent dioxygenases involved in flavonoid biosynthesis. Frontiers in Plant Science 12:701780.
Busche, M., et al., 2021. Functional characterisation of banana (Musa spp.) 2-oxoglutarate- dependent dioxygenases involved in flavonoid biosynthesis. Frontiers in Plant Science, 12: 701780.
M. Busche, et al., “Functional characterisation of banana (Musa spp.) 2-oxoglutarate- dependent dioxygenases involved in flavonoid biosynthesis”, Frontiers in Plant Science, vol. 12, 2021, : 701780.
Busche, M., Acatay, C., Martens, S., Weisshaar, B., Stracke, R.: Functional characterisation of banana (Musa spp.) 2-oxoglutarate- dependent dioxygenases involved in flavonoid biosynthesis. Frontiers in Plant Science. 12, : 701780 (2021).
Busche, Mareike, Acatay, Christopher, Martens, Stefan, Weisshaar, Bernd, and Stracke, Ralf. “Functional characterisation of banana (Musa spp.) 2-oxoglutarate- dependent dioxygenases involved in flavonoid biosynthesis”. Frontiers in Plant Science 12 (2021): 701780.
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2021-08-19T05:59:43Z
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PMID: 34484266
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Preprint: 10.1101/2021.05.03.442406

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