Self-contained and modular structured illumination microscope

Van den Eynde R, Vandenberg W, Hugelier S, Bouwens A, Hofkens J, Müller M, Dedecker P (2021)
Biomedical Optics Express 12(7): 4414-4422.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Van den Eynde, Robin; Vandenberg, Wim; Hugelier, Siewert; Bouwens, Arno; Hofkens, Johan; Müller, MarcelUniBi; Dedecker, Peter
Abstract / Bemerkung
We present a modular implementation of structured illumination microscopy (SIM) that is fast, largely self-contained and that can be added onto existing fluorescence microscopes. Our strategy, which we call HIT-SIM, can theoretically deliver well over 50 super-resolved images per second and is readily compatible with existing acquisition software packages. We provide a full technical package consisting of schematics, a list of components and an alignment scheme that provides detailed specifications and assembly instructions. We illustrate the performance of the instrument by imaging optically large samples containing sequence-specifically stained DNA fragments. (c) 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
Erscheinungsjahr
2021
Zeitschriftentitel
Biomedical Optics Express
Band
12
Ausgabe
7
Seite(n)
4414-4422
eISSN
2156-7085
Page URI
https://pub.uni-bielefeld.de/record/2956280

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Van den Eynde R, Vandenberg W, Hugelier S, et al. Self-contained and modular structured illumination microscope. Biomedical Optics Express. 2021;12(7):4414-4422.
Van den Eynde, R., Vandenberg, W., Hugelier, S., Bouwens, A., Hofkens, J., Müller, M., & Dedecker, P. (2021). Self-contained and modular structured illumination microscope. Biomedical Optics Express, 12(7), 4414-4422. https://doi.org/10.1364/BOE.423492
Van den Eynde, R., Vandenberg, W., Hugelier, S., Bouwens, A., Hofkens, J., Müller, M., and Dedecker, P. (2021). Self-contained and modular structured illumination microscope. Biomedical Optics Express 12, 4414-4422.
Van den Eynde, R., et al., 2021. Self-contained and modular structured illumination microscope. Biomedical Optics Express, 12(7), p 4414-4422.
R. Van den Eynde, et al., “Self-contained and modular structured illumination microscope”, Biomedical Optics Express, vol. 12, 2021, pp. 4414-4422.
Van den Eynde, R., Vandenberg, W., Hugelier, S., Bouwens, A., Hofkens, J., Müller, M., Dedecker, P.: Self-contained and modular structured illumination microscope. Biomedical Optics Express. 12, 4414-4422 (2021).
Van den Eynde, Robin, Vandenberg, Wim, Hugelier, Siewert, Bouwens, Arno, Hofkens, Johan, Müller, Marcel, and Dedecker, Peter. “Self-contained and modular structured illumination microscope”. Biomedical Optics Express 12.7 (2021): 4414-4422.

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PMID: 34457422
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