Role of hepcidin in oxidative stress and cell death of cultured mouse renal collecting duct cells: protection against iron and sensitization to cadmium

Probst S, Fels J, Scharner B, Wolff NA, Roussa E, van Swelm RPL, Lee W-K, Thevenod F (2021)
Archives of toxicology.

Zeitschriftenaufsatz | E-Veröff. vor dem Druck | Englisch
 
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Autor*in
Probst, Stephanie; Fels, Johannes; Scharner, Bettina; Wolff, Natascha A; Roussa, Eleni; van Swelm, Rachel P L; Lee, Wing-KeeUniBi; Thevenod, Frank
Abstract / Bemerkung
The liver hormone hepcidin regulates systemic iron homeostasis. Hepcidin is also expressed by the kidney, but exclusively in distal nephron segments. Several studies suggest hepcidin protects against kidney damage involving Fe2+ overload. The nephrotoxic non-essential metal ion Cd2+ can displace Fe2+ from cellular biomolecules, causing oxidative stress and cell death. The role of hepcidin in Fe2+ and Cd2+ toxicity was assessed in mouse renal cortical [mCCD(cl.1)] and inner medullary [mIMCD3] collecting duct cell lines. Cells were exposed to equipotent Cd2+ (0.5-5mumol/l) and/or Fe2+ (50-100mumol/l) for 4-24h. Hepcidin (Hamp1) was transiently silenced by RNAi or overexpressed by plasmid transfection. Hepcidin or catalase expression were evaluated by RT-PCR, qPCR, immunoblotting or immunofluorescence microscopy, and cell fate by MTT, apoptosis and necrosis assays. Reactive oxygen species (ROS) were detected using CellROX Green and catalase activity by fluorometry. Hepcidin upregulation protected against Fe2+-induced mIMCD3 cell death by increasing catalase activity and reducing ROS, but exacerbated Cd2+-induced catalase dysfunction, increasing ROS and cell death. Opposite effects were observed with Hamp1 siRNA. Similar to Hamp1 silencing, increased intracellular Fe2+ prevented Cd2+ damage, ROS formation and catalase disruption whereas chelation of intracellular Fe2+ with desferrioxamine augmented Cd2+ damage, corresponding to hepcidin upregulation. Comparable effects were observed in mCCD(cl.1) cells, indicating equivalent functions of renal hepcidin in different collecting duct segments. In conclusion, hepcidin likely binds Fe2+, but not Cd2+. Because Fe2+ and Cd2+ compete for functional binding sites in proteins, hepcidin affects their free metal ion pools and differentially impacts downstream processes and cell fate.
Erscheinungsjahr
2021
Zeitschriftentitel
Archives of toxicology
eISSN
1432-0738
Page URI
https://pub.uni-bielefeld.de/record/2956164

Zitieren

Probst S, Fels J, Scharner B, et al. Role of hepcidin in oxidative stress and cell death of cultured mouse renal collecting duct cells: protection against iron and sensitization to cadmium. Archives of toxicology. 2021.
Probst, S., Fels, J., Scharner, B., Wolff, N. A., Roussa, E., van Swelm, R. P. L., Lee, W. - K., et al. (2021). Role of hepcidin in oxidative stress and cell death of cultured mouse renal collecting duct cells: protection against iron and sensitization to cadmium. Archives of toxicology. https://doi.org/10.1007/s00204-021-03106-z
Probst, S., Fels, J., Scharner, B., Wolff, N. A., Roussa, E., van Swelm, R. P. L., Lee, W. - K., and Thevenod, F. (2021). Role of hepcidin in oxidative stress and cell death of cultured mouse renal collecting duct cells: protection against iron and sensitization to cadmium. Archives of toxicology.
Probst, S., et al., 2021. Role of hepcidin in oxidative stress and cell death of cultured mouse renal collecting duct cells: protection against iron and sensitization to cadmium. Archives of toxicology.
S. Probst, et al., “Role of hepcidin in oxidative stress and cell death of cultured mouse renal collecting duct cells: protection against iron and sensitization to cadmium”, Archives of toxicology, 2021.
Probst, S., Fels, J., Scharner, B., Wolff, N.A., Roussa, E., van Swelm, R.P.L., Lee, W.-K., Thevenod, F.: Role of hepcidin in oxidative stress and cell death of cultured mouse renal collecting duct cells: protection against iron and sensitization to cadmium. Archives of toxicology. (2021).
Probst, Stephanie, Fels, Johannes, Scharner, Bettina, Wolff, Natascha A, Roussa, Eleni, van Swelm, Rachel P L, Lee, Wing-Kee, and Thevenod, Frank. “Role of hepcidin in oxidative stress and cell death of cultured mouse renal collecting duct cells: protection against iron and sensitization to cadmium”. Archives of toxicology (2021).

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