Highly compact and cost-effective 2-beam super-resolution structured illumination microscope based on all-fiber optic components

Pospisil J, Wiebusch G, Fliegel K, Klima M, Huser T (2021)
Optics Express 29(8): 11833-11844.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Current super-resolution structured illumination microscopes (SR-SIM) utilize relatively expensive electro-optic components and free-space optics, resulting in large setups. Moreover, high power laser sources are required to compensate for the losses associated with generating the illumination pattern by diffractive optics. Here, we present a highly compact and flexible 2D SR-SIM microscope based on all-fiber optic components (fiberSIM). Fiber-splitters deliver the laser light to the sample resulting in the interference illumination pattern. A microelectromechanical systems (MEMS) based fiber switch performs rapid pattern rotation. The pattern phase shift is achieved by the spatial displacement of one arm of the fiber interferometer using a piezoelectric crystal. Compared with existing methods, fiberSIM is highly compact and significantly reduces the SR-SIM component cost while achieving comparable results, thus providing a route to making SR-SIM technology accessible to even more laboratories in the life sciences. (C) 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
Erscheinungsjahr
2021
Zeitschriftentitel
Optics Express
Band
29
Ausgabe
8
Seite(n)
11833-11844
eISSN
1094-4087
Page URI
https://pub.uni-bielefeld.de/record/2955120

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Pospisil J, Wiebusch G, Fliegel K, Klima M, Huser T. Highly compact and cost-effective 2-beam super-resolution structured illumination microscope based on all-fiber optic components. Optics Express. 2021;29(8):11833-11844.
Pospisil, J., Wiebusch, G., Fliegel, K., Klima, M., & Huser, T. (2021). Highly compact and cost-effective 2-beam super-resolution structured illumination microscope based on all-fiber optic components. Optics Express, 29(8), 11833-11844. https://doi.org/10.1364/OE.420592
Pospisil, J., Wiebusch, G., Fliegel, K., Klima, M., and Huser, T. (2021). Highly compact and cost-effective 2-beam super-resolution structured illumination microscope based on all-fiber optic components. Optics Express 29, 11833-11844.
Pospisil, J., et al., 2021. Highly compact and cost-effective 2-beam super-resolution structured illumination microscope based on all-fiber optic components. Optics Express, 29(8), p 11833-11844.
J. Pospisil, et al., “Highly compact and cost-effective 2-beam super-resolution structured illumination microscope based on all-fiber optic components”, Optics Express, vol. 29, 2021, pp. 11833-11844.
Pospisil, J., Wiebusch, G., Fliegel, K., Klima, M., Huser, T.: Highly compact and cost-effective 2-beam super-resolution structured illumination microscope based on all-fiber optic components. Optics Express. 29, 11833-11844 (2021).
Pospisil, Jakub, Wiebusch, Gerd, Fliegel, Karel, Klima, Milos, and Huser, Thomas. “Highly compact and cost-effective 2-beam super-resolution structured illumination microscope based on all-fiber optic components”. Optics Express 29.8 (2021): 11833-11844.

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