Identification of Microorganisms from Several Surfaces by MALDI-TOF MS: P. aeruginosa Is Leading in Biofilm Formation

Asghari E, Kiel A, Kaltschmidt B, Wortmann M, Schmidt N, Hüsgen B, Hütten A, Knabbe C, Kaltschmidt C, Kaltschmidt B (2021)
Microorganisms 9(5): 992.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
OA 2.74 MB
Abstract / Bemerkung
New ecological trends and changes in consumer behavior are known to favor biofilm formation in household appliances, increasing the need for new antimicrobial materials and surfaces. Their development requires laboratory-cultivated biofilms, or biofilm model systems (BMS), which allow for accelerated growth and offer better understanding of the underlying formation mechanisms. Here, we identified bacterial strains in wildtype biofilms from a variety of materials from domestic appliances using matrix-assisted laser desorption/ionization-time of flight mass spectroscopy (MALDI-TOF-MS). Staphylococci and pseudomonads were identified by MALDI-TOF-MS as the main genera in the habitats and were analyzed for biofilm formation using various in vitro methods. Standard quantitative biofilm assays were combined with scanning electron microscopy (SEM) to characterize biofilm formation. While Pseudomonas putida, a published lead germ, was not identified in any of the collected samples, Pseudomonas aeruginosa was found to be the most dominant biofilm producer. Water-born Pseudomonads were dominantly found in compartments with water contact only, such as in detergent compartment and detergent enemata. Furthermore, materials in contact with the washing load are predominantly colonized with bacteria from the human.
Stichworte
domestic appliances; biofilm; MALDI-TOF-MS; scanning electron microscopy; Pseudomonas aeruginosa
Erscheinungsjahr
2021
Zeitschriftentitel
Microorganisms
Band
9
Ausgabe
5
Seite(n)
992
eISSN
2076-2607
Finanzierungs-Informationen
Open-Access-Publikationskosten wurden durch die Universität Bielefeld gefördert.
Page URI
https://pub.uni-bielefeld.de/record/2954526

Zitieren

Asghari E, Kiel A, Kaltschmidt B, et al. Identification of Microorganisms from Several Surfaces by MALDI-TOF MS: P. aeruginosa Is Leading in Biofilm Formation. Microorganisms. 2021;9(5):992.
Asghari, E., Kiel, A., Kaltschmidt, B., Wortmann , M., Schmidt , N., Hüsgen, B., Hütten, A., et al. (2021). Identification of Microorganisms from Several Surfaces by MALDI-TOF MS: P. aeruginosa Is Leading in Biofilm Formation. Microorganisms, 9(5), 992. https://doi.org/10.3390/microorganisms9050992
Asghari, E., Kiel, A., Kaltschmidt, B., Wortmann , M., Schmidt , N., Hüsgen, B., Hütten, A., Knabbe, C., Kaltschmidt, C., and Kaltschmidt, B. (2021). Identification of Microorganisms from Several Surfaces by MALDI-TOF MS: P. aeruginosa Is Leading in Biofilm Formation. Microorganisms 9, 992.
Asghari, E., et al., 2021. Identification of Microorganisms from Several Surfaces by MALDI-TOF MS: P. aeruginosa Is Leading in Biofilm Formation. Microorganisms, 9(5), p 992.
E. Asghari, et al., “Identification of Microorganisms from Several Surfaces by MALDI-TOF MS: P. aeruginosa Is Leading in Biofilm Formation”, Microorganisms, vol. 9, 2021, pp. 992.
Asghari, E., Kiel, A., Kaltschmidt, B., Wortmann , M., Schmidt , N., Hüsgen, B., Hütten, A., Knabbe, C., Kaltschmidt, C., Kaltschmidt, B.: Identification of Microorganisms from Several Surfaces by MALDI-TOF MS: P. aeruginosa Is Leading in Biofilm Formation. Microorganisms. 9, 992 (2021).
Asghari, Ehsan, Kiel, Annika, Kaltschmidt, Bernhard, Wortmann , Martin, Schmidt , Nadine, Hüsgen, Bruno, Hütten, Andreas, Knabbe, Cornelius, Kaltschmidt, Christian, and Kaltschmidt, Barbara. “Identification of Microorganisms from Several Surfaces by MALDI-TOF MS: P. aeruginosa Is Leading in Biofilm Formation”. Microorganisms 9.5 (2021): 992.
Alle Dateien verfügbar unter der/den folgenden Lizenz(en):
Creative Commons Namensnennung 4.0 International Public License (CC-BY 4.0):
Volltext(e)
Access Level
OA Open Access
Zuletzt Hochgeladen
2021-05-04T23:04:35Z
MD5 Prüfsumme
cf35ef1757f8eab2417291e4e6986f46

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Suchen in

Google Scholar