Simulating digital micromirror devices for patterning coherent excitation light in structured illumination microscopy

Lachetta M, Sandmeyer H, Sandmeyer A, Schulte Am Esch J, Huser T, Müller M (2021)
Philosophical transactions. Series A, Mathematical, physical, and engineering sciences 379(2199).

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Digital micromirror devices (DMDs) are spatial light modulators that employ the electro-mechanical movement of miniaturized mirrors to steer and thus modulate the light reflected off a mirror array. Their wide availability, low cost and high speed make them a popular choice both in consumer electronics such as video projectors, and scientific applications such as microscopy. High-end fluorescence microscopy systems typically employ laser light sources, which by their nature provide coherent excitation light. In super-resolution microscopy applications that use light modulation, most notably structured illumination microscopy (SIM), the coherent nature of the excitation light becomes a requirement to achieve optimal interference pattern contrast. The universal combination of DMDs and coherent light sources, especially when working with multiple different wavelengths, is unfortunately not straight forward. The substructure of the tilted micromirror array gives rise to a blazed grating, which has to be understood and which must be taken into account when designing a DMD-based illumination system. Here, we present a set of simulation frameworks that explore the use of DMDs in conjunction with coherent light sources, motivated by their application in SIM, but which are generalizable to other light patterning applications. This framework provides all the tools to explore and compute DMD-based diffraction effects and to simulate possible system alignment configurations computationally, which simplifies the system design process and provides guidance for setting up DMD-based microscopes. This article is part of the Theo Murphy meeting 'Super-resolution structured illumination microscopy (part 1)'.
Erscheinungsjahr
2021
Zeitschriftentitel
Philosophical transactions. Series A, Mathematical, physical, and engineering sciences
Band
379
Ausgabe
2199
eISSN
1471-2962
Page URI
https://pub.uni-bielefeld.de/record/2954372

Zitieren

Lachetta M, Sandmeyer H, Sandmeyer A, Schulte Am Esch J, Huser T, Müller M. Simulating digital micromirror devices for patterning coherent excitation light in structured illumination microscopy. Philosophical transactions. Series A, Mathematical, physical, and engineering sciences. 2021;379(2199).
Lachetta, M., Sandmeyer, H., Sandmeyer, A., Schulte Am Esch, J., Huser, T., & Müller, M. (2021). Simulating digital micromirror devices for patterning coherent excitation light in structured illumination microscopy. Philosophical transactions. Series A, Mathematical, physical, and engineering sciences, 379(2199). https://doi.org/10.1098/rsta.2020.0147
Lachetta, M., Sandmeyer, H., Sandmeyer, A., Schulte Am Esch, J., Huser, T., and Müller, M. (2021). Simulating digital micromirror devices for patterning coherent excitation light in structured illumination microscopy. Philosophical transactions. Series A, Mathematical, physical, and engineering sciences 379.
Lachetta, M., et al., 2021. Simulating digital micromirror devices for patterning coherent excitation light in structured illumination microscopy. Philosophical transactions. Series A, Mathematical, physical, and engineering sciences, 379(2199).
M. Lachetta, et al., “Simulating digital micromirror devices for patterning coherent excitation light in structured illumination microscopy”, Philosophical transactions. Series A, Mathematical, physical, and engineering sciences, vol. 379, 2021.
Lachetta, M., Sandmeyer, H., Sandmeyer, A., Schulte Am Esch, J., Huser, T., Müller, M.: Simulating digital micromirror devices for patterning coherent excitation light in structured illumination microscopy. Philosophical transactions. Series A, Mathematical, physical, and engineering sciences. 379, (2021).
Lachetta, Mario, Sandmeyer, Hauke, Sandmeyer, Alice, Schulte Am Esch, Jan, Huser, Thomas, and Müller, Marcel. “Simulating digital micromirror devices for patterning coherent excitation light in structured illumination microscopy”. Philosophical transactions. Series A, Mathematical, physical, and engineering sciences 379.2199 (2021).
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2021-08-31T15:51:26Z
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