Novel Primary Human Cancer Stem-Like Cell Populations from Non-Small Cell Lung Cancer: Inhibition of Cell Survival by Targeting NF-κB and MYC Signaling

Windmöller BA, Beshay M, Helweg LP, Flottmann C, Beermann M, Förster C, Wilkens L, Greiner J, Kaltschmidt C, Kaltschmidt B (2021)
Cells 10(5): 1024.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Windmöller, Beatrice A.; Beshay, Morris; Helweg, Laureen P.; Flottmann, Clara; Beermann, Miriam; Förster, Christine; Wilkens, Ludwig; Greiner, JohannesUniBi ; Kaltschmidt, ChristianUniBi; Kaltschmidt, BarbaraUniBi
Abstract / Bemerkung
There is growing evidence that cancer stem cells (CSCs), a small subpopulation of self-renewal cancer cells, are responsible for tumor growth, treatment resistance, and cancer relapse and are thus of enormous clinical interest. Here, we aimed to isolate new CSC-like cells derived from human primary non-small cell lung cancer (NSCLC) specimens and to analyze the influence of different inhibitors of NF-κB and MYC signaling on cell survival. CSC-like cells were established from three squamous cell carcinomas (SCC) and three adenocarcinomas (AC) of the lung and were shown to express common CSC markers such as Prominin-1, CD44-antigen, and Nestin. Further, cells gave rise to spherical cancer organoids. Inhibition of MYC and NF-κB signaling using KJ-Pyr-9, dexamethasone, and pyrrolidinedithiocarbamate resulted in significant reductions in cell survival for SCC- and AC-derived cells. However, inhibition of the protein–protein interaction of MYC/NMYC proto-oncogenes with Myc-associated factor X (MAX) using KJ-Pyr-9 revealed the most promising survival-decreasing effects. Next to the establishment of six novel in vitro models for studying NSCLC-derived CSC-like populations, the presented investigations might provide new insights into potential novel therapies targeting NF-κB/MYC to improve clinical outcomes in NSCLC patients. Nevertheless, the full picture of downstream signaling still remains elusive.
Stichworte
cancer stem cell-like cells; squamous cell carcinoma; adenocarcinoma; NSCLC; MYC; NF-kB
Erscheinungsjahr
2021
Zeitschriftentitel
Cells
Band
10
Ausgabe
5
Art.-Nr.
1024
eISSN
2073-4409
Page URI
https://pub.uni-bielefeld.de/record/2954303

Zitieren

Windmöller BA, Beshay M, Helweg LP, et al. Novel Primary Human Cancer Stem-Like Cell Populations from Non-Small Cell Lung Cancer: Inhibition of Cell Survival by Targeting NF-κB and MYC Signaling. Cells. 2021;10(5): 1024.
Windmöller, B. A., Beshay, M., Helweg, L. P., Flottmann, C., Beermann, M., Förster, C., Wilkens, L., et al. (2021). Novel Primary Human Cancer Stem-Like Cell Populations from Non-Small Cell Lung Cancer: Inhibition of Cell Survival by Targeting NF-κB and MYC Signaling. Cells, 10(5), 1024. https://doi.org/10.3390/cells10051024
Windmöller, B. A., Beshay, M., Helweg, L. P., Flottmann, C., Beermann, M., Förster, C., Wilkens, L., Greiner, J., Kaltschmidt, C., and Kaltschmidt, B. (2021). Novel Primary Human Cancer Stem-Like Cell Populations from Non-Small Cell Lung Cancer: Inhibition of Cell Survival by Targeting NF-κB and MYC Signaling. Cells 10:1024.
Windmöller, B.A., et al., 2021. Novel Primary Human Cancer Stem-Like Cell Populations from Non-Small Cell Lung Cancer: Inhibition of Cell Survival by Targeting NF-κB and MYC Signaling. Cells, 10(5): 1024.
B.A. Windmöller, et al., “Novel Primary Human Cancer Stem-Like Cell Populations from Non-Small Cell Lung Cancer: Inhibition of Cell Survival by Targeting NF-κB and MYC Signaling”, Cells, vol. 10, 2021, : 1024.
Windmöller, B.A., Beshay, M., Helweg, L.P., Flottmann, C., Beermann, M., Förster, C., Wilkens, L., Greiner, J., Kaltschmidt, C., Kaltschmidt, B.: Novel Primary Human Cancer Stem-Like Cell Populations from Non-Small Cell Lung Cancer: Inhibition of Cell Survival by Targeting NF-κB and MYC Signaling. Cells. 10, : 1024 (2021).
Windmöller, Beatrice A., Beshay, Morris, Helweg, Laureen P., Flottmann, Clara, Beermann, Miriam, Förster, Christine, Wilkens, Ludwig, Greiner, Johannes, Kaltschmidt, Christian, and Kaltschmidt, Barbara. “Novel Primary Human Cancer Stem-Like Cell Populations from Non-Small Cell Lung Cancer: Inhibition of Cell Survival by Targeting NF-κB and MYC Signaling”. Cells 10.5 (2021): 1024.
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2021-04-28T11:32:47Z
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