Chitinase-Induced Airway Hyperreactivity and Inflammation in a Mouse Model of Nonallergic Asthma

Weber-Chrysochoou C, Darcan-Nicolaisen Y, Wohlgensinger J, Tinner EM, Frei R, Loeliger S, Lauener RP, Hamelmann E (2021)
International archives of allergy and immunology.

Zeitschriftenaufsatz | E-Veröff. vor dem Druck | Englisch
 
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Autor*in
Weber-Chrysochoou, Christina; Darcan-Nicolaisen, Yasemin; Wohlgensinger, Johanna; Tinner, Eva Maria; Frei, Remo; Loeliger, Susanne; Lauener, Roger P; Hamelmann, EckardUniBi
Abstract / Bemerkung
INTRODUCTION: Environmental exposure to mites and fungi has been proposed to critically contribute to the development of IgE-mediated asthma. A common denominator of such organisms is chitin. Human chitinases have been reported to be upregulated by interleukin-13 secreted in the context of Th2-type immune responses and to induce asthma. We assessed whether chitin-containing components induced chitinases in an innate immune-dependent way and whether this results in bronchial hyperresponsiveness.; MATERIALS AND METHODS: Monocyte/macrophage cell lines were stimulated with chitin-containing or bacterial components in vitro. Chitinase activity in the supernatant and the expression of the chitotriosidase gene were measured by enzyme assay and quantitative PCR, respectively. Non-sensitized mice were stimulated with chitin-containing components intranasally, and a chitinase inhibitor was administered intraperitoneally. As markers for inflammation leukocytes were counted in the bronchoalveolar lavage (BAL) fluid, and airway hyperresponsiveness was assessed via methacholine challenge.; RESULTS: We found both whole chitin-containing dust mites as well as the fungal cell wall component zymosan A but not endotoxin-induced chitinase activity and chitotriosidase gene expression in vitro. The intranasal application of zymosan A into mice led to the induction of chitinase activity in the BAL fluid and to bronchial hyperresponsiveness, which could be reduced by applying the chitinase inhibitor allosamidin.; DISCUSSION: We propose that environmental exposure to mites and fungi leads to the induction of chitinase, which in turn favors the development of bronchial hyperreactivity in an IgE-independent manner. © 2021 The Author(s) Published by S. Karger AG, Basel.
Erscheinungsjahr
2021
Zeitschriftentitel
International archives of allergy and immunology
eISSN
1423-0097
Page URI
https://pub.uni-bielefeld.de/record/2953000

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Weber-Chrysochoou C, Darcan-Nicolaisen Y, Wohlgensinger J, et al. Chitinase-Induced Airway Hyperreactivity and Inflammation in a Mouse Model of Nonallergic Asthma. International archives of allergy and immunology. 2021.
Weber-Chrysochoou, C., Darcan-Nicolaisen, Y., Wohlgensinger, J., Tinner, E. M., Frei, R., Loeliger, S., Lauener, R. P., et al. (2021). Chitinase-Induced Airway Hyperreactivity and Inflammation in a Mouse Model of Nonallergic Asthma. International archives of allergy and immunology. https://doi.org/10.1159/000513296
Weber-Chrysochoou, C., Darcan-Nicolaisen, Y., Wohlgensinger, J., Tinner, E. M., Frei, R., Loeliger, S., Lauener, R. P., and Hamelmann, E. (2021). Chitinase-Induced Airway Hyperreactivity and Inflammation in a Mouse Model of Nonallergic Asthma. International archives of allergy and immunology.
Weber-Chrysochoou, C., et al., 2021. Chitinase-Induced Airway Hyperreactivity and Inflammation in a Mouse Model of Nonallergic Asthma. International archives of allergy and immunology.
C. Weber-Chrysochoou, et al., “Chitinase-Induced Airway Hyperreactivity and Inflammation in a Mouse Model of Nonallergic Asthma”, International archives of allergy and immunology, 2021.
Weber-Chrysochoou, C., Darcan-Nicolaisen, Y., Wohlgensinger, J., Tinner, E.M., Frei, R., Loeliger, S., Lauener, R.P., Hamelmann, E.: Chitinase-Induced Airway Hyperreactivity and Inflammation in a Mouse Model of Nonallergic Asthma. International archives of allergy and immunology. (2021).
Weber-Chrysochoou, Christina, Darcan-Nicolaisen, Yasemin, Wohlgensinger, Johanna, Tinner, Eva Maria, Frei, Remo, Loeliger, Susanne, Lauener, Roger P, and Hamelmann, Eckard. “Chitinase-Induced Airway Hyperreactivity and Inflammation in a Mouse Model of Nonallergic Asthma”. International archives of allergy and immunology (2021).

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