Microglia Development and Maturation and Its Implications for Induction of Microglia-Like Cells from Human iPSCs

Wurm J, Konttinen H, Andressen C, Malm T, Spittau B (2021)
International Journal of Molecular Sciences 22(6): 3088.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Microglia are resident immune cells of the central nervous system and play critical roles during the development, homeostasis, and pathologies of the brain. Originated from yolk sac erythromyeloid progenitors, microglia immigrate into the embryonic brain parenchyma to undergo final postnatal differentiation and maturation driven by distinct chemokines, cytokines, and growth factors. Among them, TGFβ1 is an important regulator of microglial functions, mediating homeostasis, anti-inflammation, and triggering the expression of microglial homeostatic signature genes. Since microglia studies are mainly based on rodent cells and the isolation of homeostatic microglia from human tissue is challenging, human-induced pluripotent stem cells have been successfully differentiated into microglia-like cells recently. However, employed differentiation protocols strongly vary regarding used cytokines and growth factors, culture conditions, time span, and cell yield. Moreover, the incomplete differentiation of human microglia can hamper the similarity to primary human microglia and dramatically influence the outcome of follow-up studies with these differentiated cells. This review summarizes the current knowledge of the molecular mechanisms driving rodent microglia differentiation in vivo, further compares published differentiation protocols, and highlights the potential of TGFβ as an essential maturation factor.
Stichworte
microglia maturation; TGFβ; iPSC; microglia-like cells; hiMGLs
Erscheinungsjahr
2021
Zeitschriftentitel
International Journal of Molecular Sciences
Band
22
Ausgabe
6
Art.-Nr.
3088
eISSN
1422-0067
Finanzierungs-Informationen
Open-Access-Publikationskosten wurden durch die Universität Bielefeld gefördert.
Page URI
https://pub.uni-bielefeld.de/record/2952988

Zitieren

Wurm J, Konttinen H, Andressen C, Malm T, Spittau B. Microglia Development and Maturation and Its Implications for Induction of Microglia-Like Cells from Human iPSCs. International Journal of Molecular Sciences. 2021;22(6): 3088.
Wurm, J., Konttinen, H., Andressen, C., Malm, T., & Spittau, B. (2021). Microglia Development and Maturation and Its Implications for Induction of Microglia-Like Cells from Human iPSCs. International Journal of Molecular Sciences, 22(6), 3088. https://doi.org/10.3390/ijms22063088
Wurm, J., Konttinen, H., Andressen, C., Malm, T., and Spittau, B. (2021). Microglia Development and Maturation and Its Implications for Induction of Microglia-Like Cells from Human iPSCs. International Journal of Molecular Sciences 22:3088.
Wurm, J., et al., 2021. Microglia Development and Maturation and Its Implications for Induction of Microglia-Like Cells from Human iPSCs. International Journal of Molecular Sciences, 22(6): 3088.
J. Wurm, et al., “Microglia Development and Maturation and Its Implications for Induction of Microglia-Like Cells from Human iPSCs”, International Journal of Molecular Sciences, vol. 22, 2021, : 3088.
Wurm, J., Konttinen, H., Andressen, C., Malm, T., Spittau, B.: Microglia Development and Maturation and Its Implications for Induction of Microglia-Like Cells from Human iPSCs. International Journal of Molecular Sciences. 22, : 3088 (2021).
Wurm, Johannes, Konttinen, Henna, Andressen, Christian, Malm, Tarja, and Spittau, Björn. “Microglia Development and Maturation and Its Implications for Induction of Microglia-Like Cells from Human iPSCs”. International Journal of Molecular Sciences 22.6 (2021): 3088.
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2021-03-22T08:26:53Z
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