CRISPRi-library guided target identification for engineering carotenoid production by Corynebacterium glutamicum

Göttl V, Schmitt I, Braun K, Peters-Wendisch P, Wendisch VF, Henke NA (2021)
Microorganisms 9(4): 670.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Corynebacterium glutamicum is a prominent production host for various value-added compounds in white biotechnology. Gene repression by dCas9/clustered regularly interspaced short palindromic repeats (CRISPR) interference (CRISPRi) allows for the identification of target genes for metabolic engineering. In this study, a CRISPRi-based library for the repression of 74 genes of C. glutamicum was constructed. The chosen genes included genes encoding enzymes of glycolysis, the pentose phosphate pathway, and the tricarboxylic acid cycle, regulatory genes, as well as genes of the methylerythritol phosphate and carotenoid biosynthesis pathways. As expected, CRISPRi-mediated repression of the carotenogenesis repressor gene crtR resulted in increased pigmentation and cellular content of the native carotenoid pigment decaprenoxanthin. CRISPRi screening identified 14 genes that affected decaprenoxanthin biosynthesis when repressed. Carotenoid biosynthesis was significantly decreased upon CRISPRi-mediated repression of 11 of these genes, while repression of 3 genes was beneficial for decaprenoxanthin production. Largely, but not in all cases, deletion of selected genes identified in the CRISPRi screen confirmed the pigmentation phenotypes obtained by CRISPRi. Notably, deletion of pgi as well as of gapA improved decaprenoxanthin levels 43-fold and 9-fold, respectively. The scope of the designed library to identify metabolic engineering targets, transfer of gene repression to stable gene deletion, and limitations of the approach were discussed.
Stichworte
CRISPRinterference; carotenoids; CRISPRi; library; metabolicengineering; terpenoids; Corynebacteriumglutamicum
Erscheinungsjahr
2021
Zeitschriftentitel
Microorganisms
Band
9
Ausgabe
4
Art.-Nr.
670
eISSN
2076-2607
Finanzierungs-Informationen
Open-Access-Publikationskosten wurden durch die Universität Bielefeld gefördert.
Page URI
https://pub.uni-bielefeld.de/record/2952957

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Göttl V, Schmitt I, Braun K, Peters-Wendisch P, Wendisch VF, Henke NA. CRISPRi-library guided target identification for engineering carotenoid production by Corynebacterium glutamicum. Microorganisms. 2021;9(4): 670.
Göttl, V., Schmitt, I., Braun, K., Peters-Wendisch, P., Wendisch, V. F., & Henke, N. A. (2021). CRISPRi-library guided target identification for engineering carotenoid production by Corynebacterium glutamicum. Microorganisms, 9(4), 670. https://doi.org/10.3390/microorganisms9040670
Göttl, Vanessa, Schmitt, Ina, Braun, Kristina, Peters-Wendisch, Petra, Wendisch, Volker F., and Henke, Nadja Alina. 2021. “CRISPRi-library guided target identification for engineering carotenoid production by Corynebacterium glutamicum”. Microorganisms 9 (4): 670.
Göttl, V., Schmitt, I., Braun, K., Peters-Wendisch, P., Wendisch, V. F., and Henke, N. A. (2021). CRISPRi-library guided target identification for engineering carotenoid production by Corynebacterium glutamicum. Microorganisms 9:670.
Göttl, V., et al., 2021. CRISPRi-library guided target identification for engineering carotenoid production by Corynebacterium glutamicum. Microorganisms, 9(4): 670.
V. Göttl, et al., “CRISPRi-library guided target identification for engineering carotenoid production by Corynebacterium glutamicum”, Microorganisms, vol. 9, 2021, : 670.
Göttl, V., Schmitt, I., Braun, K., Peters-Wendisch, P., Wendisch, V.F., Henke, N.A.: CRISPRi-library guided target identification for engineering carotenoid production by Corynebacterium glutamicum. Microorganisms. 9, : 670 (2021).
Göttl, Vanessa, Schmitt, Ina, Braun, Kristina, Peters-Wendisch, Petra, Wendisch, Volker F., and Henke, Nadja Alina. “CRISPRi-library guided target identification for engineering carotenoid production by Corynebacterium glutamicum”. Microorganisms 9.4 (2021): 670.
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2021-03-24T14:20:46Z
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