Transcriptomic analysis of temporal shifts in berry development between two grapevine cultivars of the Pinot family reveals potential ripening-regulative genes

Theine J, Holtgräwe D, Herzog K, Schwander F, Kicherer A, Hausmann L, Viehöver P, Töpfer R, Weisshaar B (2021)
bioRxiv.

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Autor*in
Theine, JensUniBi; Holtgräwe, DanielaUniBi ; Herzog, Katja; Schwander, Florian; Kicherer, Anna; Hausmann, Ludger; Viehöver, PriscaUniBi; Töpfer, Reinhard; Weisshaar, BerndUniBi
Abstract / Bemerkung
Background Grapevine cultivars of the Pinot family represent in the broader sense clonally propagated mutants with clear-cut phenotypes, such as different color or shifted ripening time, that result in major phenotypic and physiological differences as well as changes in important viticultural traits. Specifically, the cultivars 'Pinot Noir' (PN) and 'Pinot Noir Precoce' (PNP, early ripening) flower at the same time, but vary for the beginning of berry ripening (véraison) and consequently for the harvest time. Apart from the genotype, seasonal climatic conditions (i.e. high temperatures) also affect ripening times. To reveal possible ripening-regulatory genes affecting the timing of the start of ripening, we investigated differences in gene expression profiles between PN and PNP throughout berry development with a closely meshed time series and in two years. Results The difference in the duration of berry formation between PN and PNP was quantified to be about two weeks under the growth conditions applied, using plant material with a proven clonal relationship of PN and PNP. Clusters of co-expressed genes and differentially expressed genes (DEGs) were detected which reflect the shift in the beginning of ripening at the level of gene expression profiles. Functional annotation of these DEGs fits to phenotypic and physiological changes during berry development. In total, we observed between PN and PNP 3,342 DEGs in 2014 and 2,745 DEGs in 2017. The intersection of both years comprises 1,923 DEGs. Among these, 388 DEGs were identified as véraison-specific and 12 were considered as candidates for a regulatory effect on berry ripening time. The expression profiles revealed two candidate genes for Ripening Time Control, designated VviRTIC1 and VviRTIC2 (VIT_210s0071g01145 and VIT_200s0366g00020, respectively) that may contribute to controlling the phenotypic difference between PN and PNP. Conclusions Many of the 1,923 DEGs identified show highly similar expression profiles in both cultivars as far as accelerated berry formation of PNP is concerned. Putative ripening-regulatory genes differentially expressed between PNP and PN as well as véraison-specific genes were identified. We point out potential connections of these genes to molecular events during berry development and discuss potential ripening time controlling candidate genes, two of which are already differentially expressed in the early berry development phase. Several down-regulated genes are annotated to encode auxin response factors / ARFs. Conceivably, changes in auxin signaling may realize the earlier ripening phenotype of PNP.
Erscheinungsjahr
2021
Zeitschriftentitel
bioRxiv
Page URI
https://pub.uni-bielefeld.de/record/2952955

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Theine J, Holtgräwe D, Herzog K, et al. Transcriptomic analysis of temporal shifts in berry development between two grapevine cultivars of the Pinot family reveals potential ripening-regulative genes. bioRxiv. 2021.
Theine, J., Holtgräwe, D., Herzog, K., Schwander, F., Kicherer, A., Hausmann, L., Viehöver, P., et al. (2021). Transcriptomic analysis of temporal shifts in berry development between two grapevine cultivars of the Pinot family reveals potential ripening-regulative genes. bioRxiv. https://doi.org/10.1101/2021.03.18.436038
Theine, J., Holtgräwe, D., Herzog, K., Schwander, F., Kicherer, A., Hausmann, L., Viehöver, P., Töpfer, R., and Weisshaar, B. (2021). Transcriptomic analysis of temporal shifts in berry development between two grapevine cultivars of the Pinot family reveals potential ripening-regulative genes. bioRxiv.
Theine, J., et al., 2021. Transcriptomic analysis of temporal shifts in berry development between two grapevine cultivars of the Pinot family reveals potential ripening-regulative genes. bioRxiv.
J. Theine, et al., “Transcriptomic analysis of temporal shifts in berry development between two grapevine cultivars of the Pinot family reveals potential ripening-regulative genes”, bioRxiv, 2021.
Theine, J., Holtgräwe, D., Herzog, K., Schwander, F., Kicherer, A., Hausmann, L., Viehöver, P., Töpfer, R., Weisshaar, B.: Transcriptomic analysis of temporal shifts in berry development between two grapevine cultivars of the Pinot family reveals potential ripening-regulative genes. bioRxiv. (2021).
Theine, Jens, Holtgräwe, Daniela, Herzog, Katja, Schwander, Florian, Kicherer, Anna, Hausmann, Ludger, Viehöver, Prisca, Töpfer, Reinhard, and Weisshaar, Bernd. “Transcriptomic analysis of temporal shifts in berry development between two grapevine cultivars of the Pinot family reveals potential ripening-regulative genes”. bioRxiv (2021).

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