Transcriptomic analysis of temporal shifts in berry development between two grapevine cultivars of the Pinot family reveals potential genes controlling ripening time
Theine J, Holtgräwe D, Herzog K, Schwander F, Kicherer A, Hausmann L, Viehöver P, Töpfer R, Weisshaar B (2021)
BMC Plant Biology 21: 327.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
Download
Autor*in
Theine, JensUniBi;
Holtgräwe, DanielaUniBi
;
Herzog, Katja;
Schwander, Florian;
Kicherer, Anna;
Hausmann, Ludger;
Viehöver, PriscaUniBi;
Töpfer, Reinhard;
Weisshaar, BerndUniBi ![](https://pub.uni-bielefeld.de/images/icon_orcid.png)
![](https://pub.uni-bielefeld.de/images/icon_orcid.png)
![](https://pub.uni-bielefeld.de/images/icon_orcid.png)
Einrichtung
Abstract / Bemerkung
Background
Grapevine cultivars of the Pinot family represent clonally propagated mutants with major phenotypic and physiological differences, such as different colour or shifted ripening time, as well as changes in important viticultural traits. Specifically, the cultivars ‘Pinot Noir’ (PN) and ‘Pinot Noir Precoce’ (PNP, early ripening) flower at the same time, but vary in the beginning of berry ripening (veraison) and, consequently, harvest time. In addition to genotype, seasonal climatic conditions (i.e. high temperatures) also affect ripening times. To reveal possible regulatory genes that affect the timing of veraison onset, we investigated differences in gene expression profiles between PN and PNP throughout berry development with a closely meshed time series and over two separate years.
Results
The difference in the duration of berry formation between PN and PNP was quantified to be approximately two weeks under the growth conditions applied, using plant material with a proven PN and PNP clonal relationship. Clusters of co-expressed genes and differentially expressed genes (DEGs) were detected which reflect the shift in the timing of veraison onset. Functional annotation of these DEGs fit to observed phenotypic and physiological changes during berry development. In total, we observed 3,342 DEGs in 2014 and 2,745 DEGs in 2017 between PN and PNP, with 1,923 DEGs across both years. Among these, 388 DEGs were identified as veraison-specific and 12 were considered as berry ripening time regulatory candidates. The expression profiles revealed two candidate genes for ripening time control which we designated VviRTIC1 and VviRTIC2 (VIT_210s0071g01145 and VIT_200s0366g00020, respectively). These genes likely contribute the phenotypic differences observed between PN and PNP.
Conclusions
Many of the 1,923 DEGs show highly similar expression profiles in both cultivars if the patterns are aligned according to developmental stage. In our work, putative genes differentially expressed between PNP and PN which could control ripening time as well as veraison-specific genes were identified. We point out connections of these genes to molecular events during berry development and discuss potential candidate genes which may control ripening time. Two of these candidates were observed to be differentially expressed in the early berry development phase. Several down-regulated genes during berry ripening are annotated as auxin response factors / ARFs. Conceivably, general changes in auxin signaling may cause the earlier ripening phenotype of PNP.
Erscheinungsjahr
2021
Zeitschriftentitel
BMC Plant Biology
Band
21
Art.-Nr.
327
Urheberrecht / Lizenzen
ISSN
1471-2229
Finanzierungs-Informationen
Open-Access-Publikationskosten wurden durch die Universität Bielefeld gefördert.
Page URI
https://pub.uni-bielefeld.de/record/2952955
Zitieren
Theine J, Holtgräwe D, Herzog K, et al. Transcriptomic analysis of temporal shifts in berry development between two grapevine cultivars of the Pinot family reveals potential genes controlling ripening time. BMC Plant Biology. 2021;21: 327.
Theine, J., Holtgräwe, D., Herzog, K., Schwander, F., Kicherer, A., Hausmann, L., Viehöver, P., et al. (2021). Transcriptomic analysis of temporal shifts in berry development between two grapevine cultivars of the Pinot family reveals potential genes controlling ripening time. BMC Plant Biology, 21, 327. https://doi.org/10.1186/s12870-021-03110-6
Theine, Jens, Holtgräwe, Daniela, Herzog, Katja, Schwander, Florian, Kicherer, Anna, Hausmann, Ludger, Viehöver, Prisca, Töpfer, Reinhard, and Weisshaar, Bernd. 2021. “Transcriptomic analysis of temporal shifts in berry development between two grapevine cultivars of the Pinot family reveals potential genes controlling ripening time”. BMC Plant Biology 21: 327.
Theine, J., Holtgräwe, D., Herzog, K., Schwander, F., Kicherer, A., Hausmann, L., Viehöver, P., Töpfer, R., and Weisshaar, B. (2021). Transcriptomic analysis of temporal shifts in berry development between two grapevine cultivars of the Pinot family reveals potential genes controlling ripening time. BMC Plant Biology 21:327.
Theine, J., et al., 2021. Transcriptomic analysis of temporal shifts in berry development between two grapevine cultivars of the Pinot family reveals potential genes controlling ripening time. BMC Plant Biology, 21: 327.
J. Theine, et al., “Transcriptomic analysis of temporal shifts in berry development between two grapevine cultivars of the Pinot family reveals potential genes controlling ripening time”, BMC Plant Biology, vol. 21, 2021, : 327.
Theine, J., Holtgräwe, D., Herzog, K., Schwander, F., Kicherer, A., Hausmann, L., Viehöver, P., Töpfer, R., Weisshaar, B.: Transcriptomic analysis of temporal shifts in berry development between two grapevine cultivars of the Pinot family reveals potential genes controlling ripening time. BMC Plant Biology. 21, : 327 (2021).
Theine, Jens, Holtgräwe, Daniela, Herzog, Katja, Schwander, Florian, Kicherer, Anna, Hausmann, Ludger, Viehöver, Prisca, Töpfer, Reinhard, and Weisshaar, Bernd. “Transcriptomic analysis of temporal shifts in berry development between two grapevine cultivars of the Pinot family reveals potential genes controlling ripening time”. BMC Plant Biology 21 (2021): 327.
Alle Dateien verfügbar unter der/den folgenden Lizenz(en):
Creative Commons Namensnennung 4.0 International Public License (CC-BY 4.0):
Volltext(e)
Name
Access Level
![Open access file OA](https://pub.uni-bielefeld.de/images/access_open.png)
Zuletzt Hochgeladen
2022-01-07T09:25:15Z
MD5 Prüfsumme
13d7c7dc02ad9076eb52c3526f63a18a
Link(s) zu Volltext(en)
Access Level
![Open access file OA](https://pub.uni-bielefeld.de/images/access_open.png)
Zitationen in Europe PMC
Daten bereitgestellt von Europe PubMed Central.
References
Daten bereitgestellt von Europe PubMed Central.
Export
Markieren/ Markierung löschen
Markierte Publikationen
Web of Science
Dieser Datensatz im Web of Science®Quellen
PMID: 34233614
PubMed | Europe PMC
Preprint: 10.1101/2021.03.18.436038
Suchen in