The two-component system ActJK is involved in acid stress tolerance and symbiosis in Sinorhizobium meliloti.

Albicoro FJ, Draghi WO, Martini MC, Salas ME, Torres Tejerizo GA, Lozano MJ, Lopez JL, Vacca C, Cafiero JH, Pistorio M, Bednarz H, et al. (2021)
Journal of Botechnology 329: 80-91.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Albicoro, Francisco J; Draghi, Walter O; Martini, Maria C; Salas, Maria E; Torres Tejerizo, Gonzalo A; Lozano, Mauricio J; Lopez, Jose L; Vacca, Carolina; Cafiero, Juan H; Pistorio, Mariano; Bednarz, HannaUniBi; Meier, Doreen
Alle
Abstract / Bemerkung
The nitrogen-fixing alpha-proteobacterium Sinorhizobium meliloti genome codifies at least 50 response regulator (RR) proteins mediating different and, in many cases, unknown processes. RR-mutant library screening allowed us to identify genes potentially implicated in survival to acid conditions. actJ mutation resulted in a strain with reduced growth rate under mildly acidic conditions as well as a lower capacity to tolerate a sudden shift to lethal acidic conditions compared with the parental strain. Mutation of the downstream gene actK, which encodes for a histidine kinase, showed a similar phenotype in acidic environments suggesting a functional two-component system. Interestingly, even though nodulation kinetics, quantity, and macroscopic morphology of Medicago sativa nodules were not affected in actJ and actK mutants, ActK was required to express the wild-type nitrogen fixation phenotype and ActJK was necessary for full bacteroid development and nodule occupancy. The actJK regulatory system presented here provides insights into an evolutionary process in rhizobium adaptation to acidic environments and suggests that actJK-controlled functions are crucial for optimal symbiosis development. Copyright © 2021 Elsevier B.V. All rights reserved.
Erscheinungsjahr
2021
Zeitschriftentitel
Journal of Botechnology
Band
329
Seite(n)
80-91
ISSN
1873-4863
Page URI
https://pub.uni-bielefeld.de/record/2951553

Zitieren

Albicoro FJ, Draghi WO, Martini MC, et al. The two-component system ActJK is involved in acid stress tolerance and symbiosis in Sinorhizobium meliloti. Journal of Botechnology. 2021;329:80-91.
Albicoro, F. J., Draghi, W. O., Martini, M. C., Salas, M. E., Torres Tejerizo, G. A., Lozano, M. J., Lopez, J. L., et al. (2021). The two-component system ActJK is involved in acid stress tolerance and symbiosis in Sinorhizobium meliloti. Journal of Botechnology, 329, 80-91. https://doi.org/10.1016/j.jbiotec.2021.01.006
Albicoro, F. J., Draghi, W. O., Martini, M. C., Salas, M. E., Torres Tejerizo, G. A., Lozano, M. J., Lopez, J. L., Vacca, C., Cafiero, J. H., Pistorio, M., et al. (2021). The two-component system ActJK is involved in acid stress tolerance and symbiosis in Sinorhizobium meliloti. Journal of Botechnology 329, 80-91.
Albicoro, F.J., et al., 2021. The two-component system ActJK is involved in acid stress tolerance and symbiosis in Sinorhizobium meliloti. Journal of Botechnology, 329, p 80-91.
F.J. Albicoro, et al., “The two-component system ActJK is involved in acid stress tolerance and symbiosis in Sinorhizobium meliloti.”, Journal of Botechnology, vol. 329, 2021, pp. 80-91.
Albicoro, F.J., Draghi, W.O., Martini, M.C., Salas, M.E., Torres Tejerizo, G.A., Lozano, M.J., Lopez, J.L., Vacca, C., Cafiero, J.H., Pistorio, M., Bednarz, H., Meier, D., Lagares, A., Niehaus, K., Becker, A., Del Papa, M.F.: The two-component system ActJK is involved in acid stress tolerance and symbiosis in Sinorhizobium meliloti. Journal of Botechnology. 329, 80-91 (2021).
Albicoro, Francisco J, Draghi, Walter O, Martini, Maria C, Salas, Maria E, Torres Tejerizo, Gonzalo A, Lozano, Mauricio J, Lopez, Jose L, Vacca, Carolina, Cafiero, Juan H, Pistorio, Mariano, Bednarz, Hanna, Meier, Doreen, Lagares, Antonio, Niehaus, Karsten, Becker, Anke, and Del Papa, M F. “The two-component system ActJK is involved in acid stress tolerance and symbiosis in Sinorhizobium meliloti.”. Journal of Botechnology 329 (2021): 80-91.

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