A proteomic analysis of ferulic acid metabolism in Amycolatopsis sp. ATCC 39116

Meyer F, Netzer J, Meinert C, Voigt B, Riedel K, Steinbüchel A (2018)
Applied Microbiology and Biotechnology 102(14): 6119-6142.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Meyer, FlorianUniBi ; Netzer, Julius; Meinert, Christina; Voigt, Birgit; Riedel, Katharina; Steinbüchel, Alexander
Abstract / Bemerkung
The pseudonocardiate Amycolatopsis sp. ATCC 39116 is used for the biotechnical production of natural vanillin from ferulic acid. Our laboratory has performed genetic modifications of this strain previously, but there are still many gaps in our knowledge regarding its vanillin tolerance and the general metabolism. We performed cultivations with this bacterium and compared the proteomes of stationary phase cells before ferulic acid feeding with those during ferulic acid feeding. Thereby, we identified 143 differently expressed proteins. Deletion mutants were constructed and characterized to analyze the function of nine corresponding genes. Using these mutants, we identified an active ferulic acid β-oxidation pathway and the enzymes which constitute this pathway. A combined deletion mutant in which the β-oxidation as well as non-β-oxidation pathways of ferulic acid degradation were deleted was unable to grow on ferulic acid as the sole source of carbon and energy. This mutant differs from the single deletion mutants and was unable to grow on ferulic acid. Furthermore, we showed that the non-β-oxidation pathway is involved in caffeic acid degradation; however, its deletion is complemented even in the double deletion mutant. This shows that both pathways can complement each other. The β-oxidation deletion mutant produced significantly reduced amounts of vanillic acid (0.12 instead of 0.35 g/l). Therefore, the resulting mutant could be used as an improved production strain. The quinone oxidoreductase deletion mutant (ΔytfG) degraded ferulic acid slower at first but produced comparable amounts of vanillin and significantly less vanillyl alcohol when compared to the parent strain.
Erscheinungsjahr
2018
Zeitschriftentitel
Applied Microbiology and Biotechnology
Band
102
Ausgabe
14
Seite(n)
6119-6142
ISSN
0175-7598
eISSN
1432-0614
Page URI
https://pub.uni-bielefeld.de/record/2950263

Zitieren

Meyer F, Netzer J, Meinert C, Voigt B, Riedel K, Steinbüchel A. A proteomic analysis of ferulic acid metabolism in Amycolatopsis sp. ATCC 39116. Applied Microbiology and Biotechnology. 2018;102(14):6119-6142.
Meyer, F., Netzer, J., Meinert, C., Voigt, B., Riedel, K., & Steinbüchel, A. (2018). A proteomic analysis of ferulic acid metabolism in Amycolatopsis sp. ATCC 39116. Applied Microbiology and Biotechnology, 102(14), 6119-6142. https://doi.org/10.1007/s00253-018-9061-y
Meyer, F., Netzer, J., Meinert, C., Voigt, B., Riedel, K., and Steinbüchel, A. (2018). A proteomic analysis of ferulic acid metabolism in Amycolatopsis sp. ATCC 39116. Applied Microbiology and Biotechnology 102, 6119-6142.
Meyer, F., et al., 2018. A proteomic analysis of ferulic acid metabolism in Amycolatopsis sp. ATCC 39116. Applied Microbiology and Biotechnology, 102(14), p 6119-6142.
F. Meyer, et al., “A proteomic analysis of ferulic acid metabolism in Amycolatopsis sp. ATCC 39116”, Applied Microbiology and Biotechnology, vol. 102, 2018, pp. 6119-6142.
Meyer, F., Netzer, J., Meinert, C., Voigt, B., Riedel, K., Steinbüchel, A.: A proteomic analysis of ferulic acid metabolism in Amycolatopsis sp. ATCC 39116. Applied Microbiology and Biotechnology. 102, 6119-6142 (2018).
Meyer, Florian, Netzer, Julius, Meinert, Christina, Voigt, Birgit, Riedel, Katharina, and Steinbüchel, Alexander. “A proteomic analysis of ferulic acid metabolism in Amycolatopsis sp. ATCC 39116”. Applied Microbiology and Biotechnology 102.14 (2018): 6119-6142.

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