Not Cleaving the His-tag of Thal Results in More Tightly Packed and Better-Diffracting Crystals

Moritzer A-C, Prior T, Niemann H (2020)
Crystals 10(12): 1135.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Flavin-dependent halogenases chlorinate or brominate their substrates in an environmentally friendly manner, only requiring the cofactor reduced flavin adenine dinucleotide (FADH(2)), oxygen, and halide salts. The tryptophan 6-halogenase Thal exhibits two flexible loops, which become ordered (substrate-binding loop) or adopt a closed conformation (FAD loop) upon substrate or cofactor binding. Here, we describe the structure of N-His-Thal-RebH5 containing an N-terminal His-tag from pET28a, which crystallized in a different space group (P2(1)) and, surprisingly, diffracted to a higher resolution of 1.63 angstrom than previously deposited Thal structures (P6(4); similar to 2.2 angstrom) with cleaved His-tag. Interestingly, the binding of glycine in the active site can induce an ordered conformation of the substrate-binding loop.
Stichworte
flavin-dependent halogenase; protein crystallization; tryptophan; halogenase; ThdH
Erscheinungsjahr
2020
Zeitschriftentitel
Crystals
Band
10
Ausgabe
12
Art.-Nr.
1135
eISSN
2073-4352
Page URI
https://pub.uni-bielefeld.de/record/2950229

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Moritzer A-C, Prior T, Niemann H. Not Cleaving the His-tag of Thal Results in More Tightly Packed and Better-Diffracting Crystals. Crystals. 2020;10(12): 1135.
Moritzer, A. - C., Prior, T., & Niemann, H. (2020). Not Cleaving the His-tag of Thal Results in More Tightly Packed and Better-Diffracting Crystals. Crystals, 10(12), 1135. https://doi.org/10.3390/cryst10121135
Moritzer, A. - C., Prior, T., and Niemann, H. (2020). Not Cleaving the His-tag of Thal Results in More Tightly Packed and Better-Diffracting Crystals. Crystals 10:1135.
Moritzer, A.-C., Prior, T., & Niemann, H., 2020. Not Cleaving the His-tag of Thal Results in More Tightly Packed and Better-Diffracting Crystals. Crystals, 10(12): 1135.
A.-C. Moritzer, T. Prior, and H. Niemann, “Not Cleaving the His-tag of Thal Results in More Tightly Packed and Better-Diffracting Crystals”, Crystals, vol. 10, 2020, : 1135.
Moritzer, A.-C., Prior, T., Niemann, H.: Not Cleaving the His-tag of Thal Results in More Tightly Packed and Better-Diffracting Crystals. Crystals. 10, : 1135 (2020).
Moritzer, Ann-Christin, Prior, Tina, and Niemann, Hartmut. “Not Cleaving the His-tag of Thal Results in More Tightly Packed and Better-Diffracting Crystals”. Crystals 10.12 (2020): 1135.
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2021-02-22T12:25:24Z
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