The sporothriolides. A new biosynthetic family of fungal secondary metabolites

Tian D-S, Kuhnert E, Ouazzani J, Wibberg D, Kalinowski J, Cox RJ (2020)
Chemical Science 11(46): 12477-12484.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
Es wurden keine Dateien hochgeladen. Nur Publikationsnachweis!
Autor*in
Tian, Dong-Song; Kuhnert, Eric; Ouazzani, Jamal; Wibberg, DanielUniBi; Kalinowski, JörnUniBi; Cox, Russell J.
Abstract / Bemerkung
The biosynthetic gene cluster of the antifungal metabolite sporothriolide 1 was identified from three producing ascomycetes: Hypomontagnella monticulosa MUCL 54604, H. spongiphila CLL 205 and H. submonticulosa DAOMC 242471. A transformation protocol was established, and genes encoding a fatty acid synthase subunit and a citrate synthase were simultaneously knocked out which led to loss of sporothriolide and sporochartine production. In vitro reactions showed that the sporochartines are derived from non-enzymatic Diels-Alder cycloaddition of 1 and trienylfuranol A 7 during the fermentation and extraction process. Heterologous expression of the spo genes in Aspergillus oryzae then led to the production of intermediates and shunts and delineation of a new fungal biosynthetic pathway originating in fatty acid biosynthesis. Finally, a hydrolase was revealed by in vitro studies likely contributing towards self-resistance of the producer organism.
Erscheinungsjahr
2020
Zeitschriftentitel
Chemical Science
Band
11
Ausgabe
46
Seite(n)
12477-12484
ISSN
2041-6520
eISSN
2041-6539
Page URI
https://pub.uni-bielefeld.de/record/2950214

Zitieren

Tian D-S, Kuhnert E, Ouazzani J, Wibberg D, Kalinowski J, Cox RJ. The sporothriolides. A new biosynthetic family of fungal secondary metabolites. Chemical Science. 2020;11(46):12477-12484.
Tian, D. - S., Kuhnert, E., Ouazzani, J., Wibberg, D., Kalinowski, J., & Cox, R. J. (2020). The sporothriolides. A new biosynthetic family of fungal secondary metabolites. Chemical Science, 11(46), 12477-12484. https://doi.org/10.1039/d0sc04886k
Tian, D. - S., Kuhnert, E., Ouazzani, J., Wibberg, D., Kalinowski, J., and Cox, R. J. (2020). The sporothriolides. A new biosynthetic family of fungal secondary metabolites. Chemical Science 11, 12477-12484.
Tian, D.-S., et al., 2020. The sporothriolides. A new biosynthetic family of fungal secondary metabolites. Chemical Science, 11(46), p 12477-12484.
D.-S. Tian, et al., “The sporothriolides. A new biosynthetic family of fungal secondary metabolites”, Chemical Science, vol. 11, 2020, pp. 12477-12484.
Tian, D.-S., Kuhnert, E., Ouazzani, J., Wibberg, D., Kalinowski, J., Cox, R.J.: The sporothriolides. A new biosynthetic family of fungal secondary metabolites. Chemical Science. 11, 12477-12484 (2020).
Tian, Dong-Song, Kuhnert, Eric, Ouazzani, Jamal, Wibberg, Daniel, Kalinowski, Jörn, and Cox, Russell J. “The sporothriolides. A new biosynthetic family of fungal secondary metabolites”. Chemical Science 11.46 (2020): 12477-12484.

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 34123230
PubMed | Europe PMC

Suchen in

Google Scholar