High cell density cultivation enables efficient and sustainable recombinant polyamine production in the microalga Chlamydomonas reinhardtii

Freudenberg R, Baier T, Einhaus A, Wobbe L, Kruse O (2021)
Bioresource Technology 323: 124542.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Modern chemical industry calls for new resource-efficient and sustainable value chains for production of key base chemicals such as polyamines. The green microalga Chlamydomonas reinhardtii offers great potential as an innovative green-cell factory by combining fast and inexpensive, phototrophic growth with mature genetic engineering. Here, overexpression of recombinant lysine decarboxylases in C. reinhardtii enabled the robust accumulation of the non-native polyamine cadaverine, which serves as building block for bio-polyamides. The issue of low cell densities, limiting most microalgal cultivation processes was resolved by systematically optimizing cultivation parameters. A new, easy-to-apply and fully phototrophic medium enables high cell density cultivations of C. reinhardtii with a 6-fold increase in biomass and cell count (20g/L biomass dry weight, ~2·108cells/mL). Application of high cell density cultivations in established photobioreactors resulted in a 10-fold increase of cadaverine yields, with up to 0.24g/L after 9days and maximal productivity of 0.1g/L/d. Copyright © 2020 Elsevier Ltd. All rights reserved.
Erscheinungsjahr
2021
Zeitschriftentitel
Bioresource Technology
Band
323
Art.-Nr.
124542
eISSN
1873-2976
Page URI
https://pub.uni-bielefeld.de/record/2949462

Zitieren

Freudenberg R, Baier T, Einhaus A, Wobbe L, Kruse O. High cell density cultivation enables efficient and sustainable recombinant polyamine production in the microalga Chlamydomonas reinhardtii. Bioresource Technology. 2021;323: 124542.
Freudenberg, R., Baier, T., Einhaus, A., Wobbe, L., & Kruse, O. (2021). High cell density cultivation enables efficient and sustainable recombinant polyamine production in the microalga Chlamydomonas reinhardtii. Bioresource Technology, 323, 124542. doi:10.1016/j.biortech.2020.124542
Freudenberg, R., Baier, T., Einhaus, A., Wobbe, L., and Kruse, O. (2021). High cell density cultivation enables efficient and sustainable recombinant polyamine production in the microalga Chlamydomonas reinhardtii. Bioresource Technology 323:124542.
Freudenberg, R., et al., 2021. High cell density cultivation enables efficient and sustainable recombinant polyamine production in the microalga Chlamydomonas reinhardtii. Bioresource Technology, 323: 124542.
R. Freudenberg, et al., “High cell density cultivation enables efficient and sustainable recombinant polyamine production in the microalga Chlamydomonas reinhardtii”, Bioresource Technology, vol. 323, 2021, : 124542.
Freudenberg, R., Baier, T., Einhaus, A., Wobbe, L., Kruse, O.: High cell density cultivation enables efficient and sustainable recombinant polyamine production in the microalga Chlamydomonas reinhardtii. Bioresource Technology. 323, : 124542 (2021).
Freudenberg, Robert, Baier, Thomas, Einhaus, Alexander, Wobbe, Lutz, and Kruse, Olaf. “High cell density cultivation enables efficient and sustainable recombinant polyamine production in the microalga Chlamydomonas reinhardtii”. Bioresource Technology 323 (2021): 124542.

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