Expanding the Toolbox of (R)-selective Amine Transaminases by Identification and Characterization of new Members

Telzerow A, Paris J, Hakansson M, Gonzalez-Sabin J, Rios-Lombardia N, Gröger H, Moris F, Schürmann M, Schwab H, Steiner K (2020)
Chembiochem : a European journal of chemical biology.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Telzerow, Aline; Paris, Juraj; Hakansson, Maria; Gonzalez-Sabin, Javier; Rios-Lombardia, Nicolas; Gröger, HaraldUniBi; Moris, Francisco; Schürmann, Martin; Schwab, Helmut; Steiner, Kerstin
Abstract / Bemerkung
Amine transaminases (ATAs) are used to synthesize enantiomerically pure amines, which are building blocks for pharmaceuticals and agrochemicals. (R) -selective ATAs belong to the fold type IV PLP dependent enzymes and different sequence-, structure- and substrate scope-based features have been identified in the past decade. However, our knowledge is still restricted due to the limited number of characterized (R) -ATAs with additional bias towards fungal origin. We aimed to expand the toolbox of (R) -ATAs and contribute to the understanding of this enzyme subfamily. We identified and characterized four new (R) -ATAs. The ATA from Exophiala sideris contains a motif characteristic for D-ATAs, which was previously believed to be a disqualifying factor for (R) -ATA activity. The crystal structure of the ATA from Shinella is the first from a gram-negative bacterium. The ATAs from Pseudonocardia acaciae and Tetrasphaera japonica are the first characterized (R) -ATAs with a shortened/missing N-terminal helix. The active site charges vary significantly between the new and known ATAs correlating with their diverging substrate scope. © 2020 Wiley-VCH GmbH.
Erscheinungsjahr
2020
Zeitschriftentitel
Chembiochem : a European journal of chemical biology
eISSN
1439-7633
Page URI
https://pub.uni-bielefeld.de/record/2949435

Zitieren

Telzerow A, Paris J, Hakansson M, et al. Expanding the Toolbox of (R)-selective Amine Transaminases by Identification and Characterization of new Members. Chembiochem : a European journal of chemical biology. 2020.
Telzerow, A., Paris, J., Hakansson, M., Gonzalez-Sabin, J., Rios-Lombardia, N., Gröger, H., Moris, F., et al. (2020). Expanding the Toolbox of (R)-selective Amine Transaminases by Identification and Characterization of new Members. Chembiochem : a European journal of chemical biology. doi:10.1002/cbic.202000692
Telzerow, A., Paris, J., Hakansson, M., Gonzalez-Sabin, J., Rios-Lombardia, N., Gröger, H., Moris, F., Schürmann, M., Schwab, H., and Steiner, K. (2020). Expanding the Toolbox of (R)-selective Amine Transaminases by Identification and Characterization of new Members. Chembiochem : a European journal of chemical biology.
Telzerow, A., et al., 2020. Expanding the Toolbox of (R)-selective Amine Transaminases by Identification and Characterization of new Members. Chembiochem : a European journal of chemical biology.
A. Telzerow, et al., “Expanding the Toolbox of (R)-selective Amine Transaminases by Identification and Characterization of new Members”, Chembiochem : a European journal of chemical biology, 2020.
Telzerow, A., Paris, J., Hakansson, M., Gonzalez-Sabin, J., Rios-Lombardia, N., Gröger, H., Moris, F., Schürmann, M., Schwab, H., Steiner, K.: Expanding the Toolbox of (R)-selective Amine Transaminases by Identification and Characterization of new Members. Chembiochem : a European journal of chemical biology. (2020).
Telzerow, Aline, Paris, Juraj, Hakansson, Maria, Gonzalez-Sabin, Javier, Rios-Lombardia, Nicolas, Gröger, Harald, Moris, Francisco, Schürmann, Martin, Schwab, Helmut, and Steiner, Kerstin. “Expanding the Toolbox of (R)-selective Amine Transaminases by Identification and Characterization of new Members”. Chembiochem : a European journal of chemical biology (2020).

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