Oxygen consumption rate of Caenorhabditis elegans as a high-throughput endpoint of toxicity testing using the Seahorse XF(e)96 Extracellular Flux Analyzer

Du Preez G, Fourie H, Daneel M, Miller H, Höss S, Ricci C, Engelbrecht G, Zouhar M, Wepener V (2020)
Scientific Reports 10(1): 4239.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Du Preez, G.; Fourie, H.; Daneel, M.; Miller, H.; Höss, SebastianUniBi; Ricci, C.; Engelbrecht, G.; Zouhar, M.; Wepener, V
Abstract / Bemerkung
Caenorhabditis elegans presents functioning, biologically relevant phenotypes and is frequently used as a bioindicator of toxicity. However, most C. elegans in vivo effect-assessment methods are laborious and time consuming. Therefore, we developed a novel method to measure the oxygen consumption rate of C. elegans as a sublethal endpoint of toxicity. This protocol was tested by exposing 50 larval stage one C. elegans individuals for 48h (at 20 degrees C) to different concentrations of two toxicants i.e. benzylcetyldimethylammonium chloride (BAC-C16) and cadmium (Cd). Following exposures, the oxygen consumption rate of the C. elegans individuals were measured using the high-throughput functionality of the Seahorse XF(e)96 Extracellular Flux Analyzer. Dose-response curves for BAC-C16 (R-2=0.93; P=0.001) and Cd (R-2=0.98; P=0.001) were created. Furthermore, a strong, positive correlation was evidenced between C. elegans oxygen consumption rate and a commonly used, ecologically relevant endpoint of toxicity (growth inhibition) for BAC-C16 (R-2=0.93; P=0.0001) and Cd (R-2=0.91; P=0.0001). The data presented in this study show that C. elegans oxygen consumption rate can be used as a promising functional measurement of toxicity.
Erscheinungsjahr
2020
Zeitschriftentitel
Scientific Reports
Band
10
Ausgabe
1
Art.-Nr.
4239
eISSN
2045-2322
Page URI
https://pub.uni-bielefeld.de/record/2945623

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Du Preez G, Fourie H, Daneel M, et al. Oxygen consumption rate of Caenorhabditis elegans as a high-throughput endpoint of toxicity testing using the Seahorse XF(e)96 Extracellular Flux Analyzer. Scientific Reports. 2020;10(1): 4239.
Du Preez, G., Fourie, H., Daneel, M., Miller, H., Höss, S., Ricci, C., Engelbrecht, G., et al. (2020). Oxygen consumption rate of Caenorhabditis elegans as a high-throughput endpoint of toxicity testing using the Seahorse XF(e)96 Extracellular Flux Analyzer. Scientific Reports, 10(1), 4239. doi:10.1038/s41598-020-61054-7
Du Preez, G., Fourie, H., Daneel, M., Miller, H., Höss, S., Ricci, C., Engelbrecht, G., Zouhar, M., and Wepener, V. (2020). Oxygen consumption rate of Caenorhabditis elegans as a high-throughput endpoint of toxicity testing using the Seahorse XF(e)96 Extracellular Flux Analyzer. Scientific Reports 10:4239.
Du Preez, G., et al., 2020. Oxygen consumption rate of Caenorhabditis elegans as a high-throughput endpoint of toxicity testing using the Seahorse XF(e)96 Extracellular Flux Analyzer. Scientific Reports, 10(1): 4239.
G. Du Preez, et al., “Oxygen consumption rate of Caenorhabditis elegans as a high-throughput endpoint of toxicity testing using the Seahorse XF(e)96 Extracellular Flux Analyzer”, Scientific Reports, vol. 10, 2020, : 4239.
Du Preez, G., Fourie, H., Daneel, M., Miller, H., Höss, S., Ricci, C., Engelbrecht, G., Zouhar, M., Wepener, V.: Oxygen consumption rate of Caenorhabditis elegans as a high-throughput endpoint of toxicity testing using the Seahorse XF(e)96 Extracellular Flux Analyzer. Scientific Reports. 10, : 4239 (2020).
Du Preez, G., Fourie, H., Daneel, M., Miller, H., Höss, Sebastian, Ricci, C., Engelbrecht, G., Zouhar, M., and Wepener, V. “Oxygen consumption rate of Caenorhabditis elegans as a high-throughput endpoint of toxicity testing using the Seahorse XF(e)96 Extracellular Flux Analyzer”. Scientific Reports 10.1 (2020): 4239.

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