PUB - Publikationen an der Universität Bielefeld

The biocatalytic oxidation of amino acids represents an attractive approach towards the synthesis of alpha-keto acids, which are interest for various industrial applications. As l-amino acids are readily available from fermentation processes, these natural amino acids can serve as substrates in combination with an L-amino acid oxidase. Besides an aqueous phase as reaction medium, a further advantage of such a process is the utilization of air as oxidation agent. In this study, we studied the organic-synthetic properties of a literature-known recombinant L-amino acid oxidase from the fungus Hebeloma cylindrosporum with respect to its suitability to catalyze the formation of alphaketo acids exemplified for the synthesis of phenylpyruvic acid starting from L-phenylalanine as a substrate. In our study the enzyme displayed a reasonable operational stability in the reaction system and as well as promising applicability data with respect to substrate and product inhibition. In a biotransformation, 20mM of substrate were converted after 4hours reaction. The formation of undesired by-products was suppressed using a commercially available catalase enzyme. Copyright © 2020. Published by Elsevier B.V.