Process properties of an l-amino acid oxidase from Hebeloma cylindro-sporum for the synthesis of phenylpyruvic acid from l-phenylalanine.

Oike K, Gröger H (2020)
Journal of biotechnology.

Zeitschriftenaufsatz | E-Veröff. vor dem Druck | Englisch
 
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Abstract / Bemerkung
The biocatalytic oxidation of amino acids represents an attractive approach towards the synthesis of alpha-keto acids, which are interest for various industrial applications. As l-amino acids are readily available from fermentation processes, these natural amino acids can serve as substrates in combination with an L-amino acid oxidase. Besides an aqueous phase as reaction medium, a further advantage of such a process is the utilization of air as oxidation agent. In this study, we studied the organic-synthetic properties of a literature-known recombinant L-amino acid oxidase from the fungus Hebeloma cylindrosporum with respect to its suitability to catalyze the formation of alphaketo acids exemplified for the synthesis of phenylpyruvic acid starting from L-phenylalanine as a substrate. In our study the enzyme displayed a reasonable operational stability in the reaction system and as well as promising applicability data with respect to substrate and product inhibition. In a biotransformation, 20mM of substrate were converted after 4hours reaction. The formation of undesired by-products was suppressed using a commercially available catalase enzyme. Copyright © 2020. Published by Elsevier B.V.
Erscheinungsjahr
2020
Zeitschriftentitel
Journal of biotechnology
eISSN
1873-4863
Page URI
https://pub.uni-bielefeld.de/record/2944998

Zitieren

Oike K, Gröger H. Process properties of an l-amino acid oxidase from Hebeloma cylindro-sporum for the synthesis of phenylpyruvic acid from l-phenylalanine. Journal of biotechnology. 2020.
Oike, K., & Gröger, H. (2020). Process properties of an l-amino acid oxidase from Hebeloma cylindro-sporum for the synthesis of phenylpyruvic acid from l-phenylalanine. Journal of biotechnology. doi:10.1016/j.jbiotec.2020.07.005
Oike, K., and Gröger, H. (2020). Process properties of an l-amino acid oxidase from Hebeloma cylindro-sporum for the synthesis of phenylpyruvic acid from l-phenylalanine. Journal of biotechnology.
Oike, K., & Gröger, H., 2020. Process properties of an l-amino acid oxidase from Hebeloma cylindro-sporum for the synthesis of phenylpyruvic acid from l-phenylalanine. Journal of biotechnology.
K. Oike and H. Gröger, “Process properties of an l-amino acid oxidase from Hebeloma cylindro-sporum for the synthesis of phenylpyruvic acid from l-phenylalanine.”, Journal of biotechnology, 2020.
Oike, K., Gröger, H.: Process properties of an l-amino acid oxidase from Hebeloma cylindro-sporum for the synthesis of phenylpyruvic acid from l-phenylalanine. Journal of biotechnology. (2020).
Oike, Keiko, and Gröger, Harald. “Process properties of an l-amino acid oxidase from Hebeloma cylindro-sporum for the synthesis of phenylpyruvic acid from l-phenylalanine.”. Journal of biotechnology (2020).

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