Nematodes as bioindicators of polluted sediments using metabarcoding and microscopic taxonomy

Schenk J, Höss S, Brinke M, Kleinbölting N, Brüchner-Hüttemann H, Traunspurger W (2020)
Environment International 143: 105922.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
OA 1.22 MB
Abstract / Bemerkung
The use of bioindicator species is a widely applied approach to evaluate ecological conditions, and several indices have been designed for this purpose. To assess the impact of pollution, especially in sediments, a pollution-sensitive index based on nematodes, one of the most abundant and species-rich groups of metazoa, was developed. The NemaSPEAR[%] index in its original form relies on the morphological inspection of nematode species. The application of a morphologically based NemaSPEAR[%] at the genus-level was previously validated. The present study evaluated a NemaSPEAR[%] index based on metabarcoding of nematode communities and tested the potential of fragments from the 28S rDNA, 18S rDNA and cytochrome c oxidase subunit I (COI) genes. In general, molecular-based results tended to show a poorer condition than morphology-based results for the investigated sites. At the genus level, NemaSPEAR[%] values based on morphological data strongly correlated with those based on molecular data for both the 28S rDNA and the 18S rDNA gene fragments (R2 = 0.86 and R2 = 0.74, respectively). Within the dominant genera (>3%) identified by morphology, 68% were detected by at least one of the two ribosomal markers. At the species level, however, concordance was less pronounced, as there were several deviations of the molecular from the morphological data. These differences could mostly be attributed to shortcomings in the reference database used in the molecular-based assignments. Our pilot study shows that a molecularly based, genus-level NemaSPEAR[%] can be successfully applied to evaluate polluted sediment. Future studies need to validate this approach further, e.g. with bulk extractions of whole meiofaunal communities in order to circumvent time-consuming nematode isolation. Further database curation with abundant NemaSPEAR[%] species will also increase the applicability of this approach.
Stichworte
General Environmental Science
Erscheinungsjahr
2020
Zeitschriftentitel
Environment International
Band
143
Art.-Nr.
105922
ISSN
0160-4120
Finanzierungs-Informationen
Open-Access-Publikationskosten wurden durch die Deutsche Forschungsgemeinschaft und die Universität Bielefeld gefördert.
Page URI
https://pub.uni-bielefeld.de/record/2944633

Zitieren

Schenk J, Höss S, Brinke M, Kleinbölting N, Brüchner-Hüttemann H, Traunspurger W. Nematodes as bioindicators of polluted sediments using metabarcoding and microscopic taxonomy. Environment International. 2020;143: 105922.
Schenk, J., Höss, S., Brinke, M., Kleinbölting, N., Brüchner-Hüttemann, H., & Traunspurger, W. (2020). Nematodes as bioindicators of polluted sediments using metabarcoding and microscopic taxonomy. Environment International, 143, 105922. https://doi.org/10.1016/j.envint.2020.105922
Schenk, Janina, Höss, Sebastian, Brinke, Marvin, Kleinbölting, Nils, Brüchner-Hüttemann, Henrike, and Traunspurger, Walter. 2020. “Nematodes as bioindicators of polluted sediments using metabarcoding and microscopic taxonomy”. Environment International 143: 105922.
Schenk, J., Höss, S., Brinke, M., Kleinbölting, N., Brüchner-Hüttemann, H., and Traunspurger, W. (2020). Nematodes as bioindicators of polluted sediments using metabarcoding and microscopic taxonomy. Environment International 143:105922.
Schenk, J., et al., 2020. Nematodes as bioindicators of polluted sediments using metabarcoding and microscopic taxonomy. Environment International, 143: 105922.
J. Schenk, et al., “Nematodes as bioindicators of polluted sediments using metabarcoding and microscopic taxonomy”, Environment International, vol. 143, 2020, : 105922.
Schenk, J., Höss, S., Brinke, M., Kleinbölting, N., Brüchner-Hüttemann, H., Traunspurger, W.: Nematodes as bioindicators of polluted sediments using metabarcoding and microscopic taxonomy. Environment International. 143, : 105922 (2020).
Schenk, Janina, Höss, Sebastian, Brinke, Marvin, Kleinbölting, Nils, Brüchner-Hüttemann, Henrike, and Traunspurger, Walter. “Nematodes as bioindicators of polluted sediments using metabarcoding and microscopic taxonomy”. Environment International 143 (2020): 105922.
Alle Dateien verfügbar unter der/den folgenden Lizenz(en):
Creative Commons Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International (CC BY-NC-ND 4.0):
Volltext(e)
Access Level
OA Open Access
Zuletzt Hochgeladen
2020-07-13T06:37:55Z
MD5 Prüfsumme
380b3de3162af6ab299f9357d5d0e042


Material in PUB:
Teil dieser Dissertation
Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®
Quellen

PMID: 32663713
PubMed | Europe PMC

Suchen in

Google Scholar