Tissue- and development-stage-specific mRNA and heterogeneous CNV signatures of human ribosomal proteins in normal and cancer samples.

Panda A, Yadav A, Yeerna H, Singh A, Biehl M, Lux M, Schulz A, Klecha T, Doniach S, Khiabanian H, Ganesan S, et al. (2020)
Nucleic acids research.

Zeitschriftenaufsatz | E-Veröff. vor dem Druck | Englisch
 
Download
Es wurden keine Dateien hochgeladen. Nur Publikationsnachweis!
Autor*in
Panda, Anshuman; Yadav, Anupama; Yeerna, Huwate; Singh, Amartya; Biehl, Michael; Lux, MarkusUniBi; Schulz, AlexanderUniBi ; Klecha, Tyler; Doniach, Sebastian; Khiabanian, Hossein; Ganesan, Shridar; Tamayo, Pablo
Alle
Abstract / Bemerkung
We give results from a detailed analysis of human Ribosomal Protein (RP) levels in normal and cancer samples and cell lines from large mRNA, copy number variation and ribosome profiling datasets. After normalizing total RP mRNA levels per sample, we find highly consistent tissue specific RP mRNA signatures in normal and tumor samples. Multiple RP mRNA-subtypes exist in several cancers, with significant survival and genomic differences. Some RP mRNA variations among subtypes correlate with copy number loss of RP genes. In kidney cancer, RP subtypes map to molecular subtypes related to cell-of-origin. Pan-cancer analysis of TCGA data showed widespread single/double copy loss of RP genes, without significantly affecting survival. In several cancer cell lines, CRISPR-Cas9 knockout of RP genes did not affect cell viability. Matched RP ribosome profiling and mRNA data in humans and rodents stratified by tissue and development stage and were strongly correlated, showing that RP translation rates were proportional to mRNA levels. In a small dataset of human adult and fetal tissues, RP protein levels showed development stage and tissue specific heterogeneity of RP levels. Our results suggest that heterogeneous RP levels play a significant functional role in cellular physiology, in both normal and disease states. © The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.
Erscheinungsjahr
2020
Zeitschriftentitel
Nucleic acids research
eISSN
1362-4962
Page URI
https://pub.uni-bielefeld.de/record/2944327

Zitieren

Panda A, Yadav A, Yeerna H, et al. Tissue- and development-stage-specific mRNA and heterogeneous CNV signatures of human ribosomal proteins in normal and cancer samples. Nucleic acids research. 2020.
Panda, A., Yadav, A., Yeerna, H., Singh, A., Biehl, M., Lux, M., Schulz, A., et al. (2020). Tissue- and development-stage-specific mRNA and heterogeneous CNV signatures of human ribosomal proteins in normal and cancer samples. Nucleic acids research. doi:10.1093/nar/gkaa485
Panda, A., Yadav, A., Yeerna, H., Singh, A., Biehl, M., Lux, M., Schulz, A., Klecha, T., Doniach, S., Khiabanian, H., et al. (2020). Tissue- and development-stage-specific mRNA and heterogeneous CNV signatures of human ribosomal proteins in normal and cancer samples. Nucleic acids research.
Panda, A., et al., 2020. Tissue- and development-stage-specific mRNA and heterogeneous CNV signatures of human ribosomal proteins in normal and cancer samples. Nucleic acids research.
A. Panda, et al., “Tissue- and development-stage-specific mRNA and heterogeneous CNV signatures of human ribosomal proteins in normal and cancer samples.”, Nucleic acids research, 2020.
Panda, A., Yadav, A., Yeerna, H., Singh, A., Biehl, M., Lux, M., Schulz, A., Klecha, T., Doniach, S., Khiabanian, H., Ganesan, S., Tamayo, P., Bhanot, G.: Tissue- and development-stage-specific mRNA and heterogeneous CNV signatures of human ribosomal proteins in normal and cancer samples. Nucleic acids research. (2020).
Panda, Anshuman, Yadav, Anupama, Yeerna, Huwate, Singh, Amartya, Biehl, Michael, Lux, Markus, Schulz, Alexander, Klecha, Tyler, Doniach, Sebastian, Khiabanian, Hossein, Ganesan, Shridar, Tamayo, Pablo, and Bhanot, Gyan. “Tissue- and development-stage-specific mRNA and heterogeneous CNV signatures of human ribosomal proteins in normal and cancer samples.”. Nucleic acids research (2020).

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Quellen

PMID: 32525984
PubMed | Europe PMC

Suchen in

Google Scholar