Methanol-essential growth of Corynebacterium glutamicum: Adaptive laboratory evolution overcomes limitation due to methanethiol assimilation pathway

Hennig G, Haupka C, Fernandes de Brito L, Rückert C, Cahoreau E, Heux S, Wendisch VF (2020)
International Journal of Molecular Sciences (IJMS) 21(10): 3617.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Methanol is a sustainable substrate for biotechnology. In addition to natural methylotrophs, metabolic engineering has gained attention for transfer of methylotrophy. Here, we engineered Corynebacterium glutamicum for methanol-dependent growth with a sugar co-substrate. Heterologous expression of genes for methanol dehydrogenase from Bacillus methanolicus and of ribulose monophosphate pathway genes for hexulose phosphate synthase and isomerase from Bacillus subtilis enabled methanol-dependent growth of mutants carrying one of two independent metabolic cut-offs, i.e., either lacking ribose-5-phosphate isomerase or ribulose-5-phosphate epimerase. Whole genome sequencing of strains selected by adaptive laboratory evolution (ALE) for faster methanol-dependent growth was performed. Subsequently, three mutations were identified that caused improved methanol-dependent growth by (1) increased plasmid copy numbers, (2) enhanced riboflavin supply and (3) reduced formation of the methionine-analogue O-methyl-homoserine in the methanethiol pathway. Our findings serve as a foundation for the engineering of C. glutamicum to unleash the full potential of methanol as a carbon source in biotechnological processes.
Stichworte
synthetic methylotrophy; methanol; ribulose monophosphate pathway; adaptive laboratory evolution; isotopic labeling; metabolic engineering
Erscheinungsjahr
2020
Zeitschriftentitel
International Journal of Molecular Sciences (IJMS)
Band
21
Ausgabe
10
Art.-Nr.
3617
eISSN
1661-6596
Page URI
https://pub.uni-bielefeld.de/record/2943451

Zitieren

Hennig G, Haupka C, Fernandes de Brito L, et al. Methanol-essential growth of Corynebacterium glutamicum: Adaptive laboratory evolution overcomes limitation due to methanethiol assimilation pathway. International Journal of Molecular Sciences (IJMS) . 2020;21(10): 3617.
Hennig, G., Haupka, C., Fernandes de Brito, L., Rückert, C., Cahoreau, E., Heux, S., & Wendisch, V. F. (2020). Methanol-essential growth of Corynebacterium glutamicum: Adaptive laboratory evolution overcomes limitation due to methanethiol assimilation pathway. International Journal of Molecular Sciences (IJMS) , 21(10), 3617. doi:10.3390/ijms21103617
Hennig, G., Haupka, C., Fernandes de Brito, L., Rückert, C., Cahoreau, E., Heux, S., and Wendisch, V. F. (2020). Methanol-essential growth of Corynebacterium glutamicum: Adaptive laboratory evolution overcomes limitation due to methanethiol assimilation pathway. International Journal of Molecular Sciences (IJMS) 21:3617.
Hennig, G., et al., 2020. Methanol-essential growth of Corynebacterium glutamicum: Adaptive laboratory evolution overcomes limitation due to methanethiol assimilation pathway. International Journal of Molecular Sciences (IJMS) , 21(10): 3617.
G. Hennig, et al., “Methanol-essential growth of Corynebacterium glutamicum: Adaptive laboratory evolution overcomes limitation due to methanethiol assimilation pathway”, International Journal of Molecular Sciences (IJMS) , vol. 21, 2020, : 3617.
Hennig, G., Haupka, C., Fernandes de Brito, L., Rückert, C., Cahoreau, E., Heux, S., Wendisch, V.F.: Methanol-essential growth of Corynebacterium glutamicum: Adaptive laboratory evolution overcomes limitation due to methanethiol assimilation pathway. International Journal of Molecular Sciences (IJMS) . 21, : 3617 (2020).
Hennig, Guido, Haupka, Carsten, Fernandes de Brito, Luciana, Rückert, Christian, Cahoreau, Edern, Heux, Stéphanie, and Wendisch, Volker F. “Methanol-essential growth of Corynebacterium glutamicum: Adaptive laboratory evolution overcomes limitation due to methanethiol assimilation pathway”. International Journal of Molecular Sciences (IJMS) 21.10 (2020): 3617.
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2020-06-16T11:03:24Z
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