Vacuolar sucrose homeostasis is critical for plant development, seed properties, and nighttime survival in Arabidopsis.

Vu DP, Martins Rodrigues C, Jung B, Meissner G, Klemens PAW, Holtgräwe D, Furtauer L, Nagele T, Nieberl P, Pommerrenig B, Neuhaus HE (2020)
Journal of experimental botany.

Zeitschriftenaufsatz | E-Veröff. vor dem Druck | Englisch
 
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Autor*in
Vu, Duc Phuong; Martins Rodrigues, Cristina; Jung, Benjamin; Meissner, Garvin; Klemens, Patrick A W; Holtgräwe, DanielaUniBi ; Furtauer, Lisa; Nagele, Thomas; Nieberl, Petra; Pommerrenig, Benjamin; Neuhaus, H Ekkehard
Abstract / Bemerkung
Most cellular sucrose is present in the cytosol and vacuoles of plant cells; however, little is known about the effect of this sucrose compartmentation on plant properties. Here, we examined the effects of altered intracellular sucrose compartmentation in Arabidopsis thaliana leaves by heterologously expressing the sugar beet (Beta vulgaris) vacuolar sucrose loader BvTST2.1 and by generating lines with reduced vacuolar invertase activity (amiR vi1-2). Heterologous expression of BvTST2.1 led to increased monosaccharide levels in leaves, whereas sucrose levels remained constant, indicating that vacuolar invertase activity in mesophyll vacuoles exceeds sucrose uptake. This notion was supported by analysis of tobacco (Nicotiana benthamiana) leaves transiently expressing BvTST2.1 and the invertase inhibitor NbVIF. However, sucrose levels were strongly elevated in leaf extracts from amiR vi1-2 lines, and experiments confirmed that sucrose accumulated in the corresponding vacuoles. The amiR vi1-2 lines exhibited impaired early development and reduced seed weight. When germinated in the dark, amiR vi1-2 seedlings were less able to convert sucrose into monosaccharides than the wild type. Cold temperatures strongly downregulated both VI genes, but the amiRvi1-2 lines showed normal frost tolerance. These observations indicate that increased vacuolar sucrose levels fully compensate for the effects of low monosaccharide concentrations on frost tolerance. © The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.
Erscheinungsjahr
2020
Zeitschriftentitel
Journal of experimental botany
eISSN
1460-2431
Page URI
https://pub.uni-bielefeld.de/record/2943420

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Vu DP, Martins Rodrigues C, Jung B, et al. Vacuolar sucrose homeostasis is critical for plant development, seed properties, and nighttime survival in Arabidopsis. Journal of experimental botany. 2020.
Vu, D. P., Martins Rodrigues, C., Jung, B., Meissner, G., Klemens, P. A. W., Holtgräwe, D., Furtauer, L., et al. (2020). Vacuolar sucrose homeostasis is critical for plant development, seed properties, and nighttime survival in Arabidopsis. Journal of experimental botany. doi:10.1093/jxb/eraa205
Vu, D. P., Martins Rodrigues, C., Jung, B., Meissner, G., Klemens, P. A. W., Holtgräwe, D., Furtauer, L., Nagele, T., Nieberl, P., Pommerrenig, B., et al. (2020). Vacuolar sucrose homeostasis is critical for plant development, seed properties, and nighttime survival in Arabidopsis. Journal of experimental botany.
Vu, D.P., et al., 2020. Vacuolar sucrose homeostasis is critical for plant development, seed properties, and nighttime survival in Arabidopsis. Journal of experimental botany.
D.P. Vu, et al., “Vacuolar sucrose homeostasis is critical for plant development, seed properties, and nighttime survival in Arabidopsis.”, Journal of experimental botany, 2020.
Vu, D.P., Martins Rodrigues, C., Jung, B., Meissner, G., Klemens, P.A.W., Holtgräwe, D., Furtauer, L., Nagele, T., Nieberl, P., Pommerrenig, B., Neuhaus, H.E.: Vacuolar sucrose homeostasis is critical for plant development, seed properties, and nighttime survival in Arabidopsis. Journal of experimental botany. (2020).
Vu, Duc Phuong, Martins Rodrigues, Cristina, Jung, Benjamin, Meissner, Garvin, Klemens, Patrick A W, Holtgräwe, Daniela, Furtauer, Lisa, Nagele, Thomas, Nieberl, Petra, Pommerrenig, Benjamin, and Neuhaus, H Ekkehard. “Vacuolar sucrose homeostasis is critical for plant development, seed properties, and nighttime survival in Arabidopsis.”. Journal of experimental botany (2020).

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