Formulating bacterial endophyte: Pre-conditioning of cells and the encapsulation in amidated pectin beads.

Cruz Barrera M, Jakobs-Schönwandt D, Gomez MI, Serrato J, Ruppel S, Patel AV (2020)
Biotechnology reports (Amsterdam, Netherlands) 26: e00463.

Zeitschriftenaufsatz | E-Veröff. vor dem Druck | Englisch
 
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Autor*in
Cruz Barrera, Mauricio; Jakobs-Schönwandt, DésiréeUniBi; Gomez, Martha Isabel; Serrato, Juan; Ruppel, Silke; Patel, Anant V
Abstract / Bemerkung
Despite the benefits of bacterial endophytes, recent studies on the mostly Gram-negative bacteria lack of regard for formulation strategies. The encapsulation into biopolymeric materials such as amidated pectins hydrogels is a suitable alternative. Here, this research aimed at supporting the capability of the plant growth-promoting bacteria Kosakonia radicincitans DSM16656T to endophytically colonize plant seedlings. In this approach, the pre-conditioned cells through osmoadaptation and hydroxyectoine accumulation were used. In general, pre-osmoadapted and hydroxyectoine-supplemented bacteria cells formulated in amidated pectin dried beads increased the endophytic activity by 10-fold. Moreover, plant promotion in radish plants enhanced by 18.9% and 20.7% for a dry matter of tuber and leaves. Confocal microscopy studies with GFP-tagged bacteria revealed that bacterial aggregates formed during the activation of beads play an essential role in early colonization stages. This research encourages the integration of fermentation and formulation strategies in a bioprocess engineering approach for exploiting endophytic bacteria. © 2020 The Authors.
Erscheinungsjahr
2020
Zeitschriftentitel
Biotechnology reports (Amsterdam, Netherlands)
Band
26
Art.-Nr.
e00463
eISSN
2215-017X
Page URI
https://pub.uni-bielefeld.de/record/2943409

Zitieren

Cruz Barrera M, Jakobs-Schönwandt D, Gomez MI, Serrato J, Ruppel S, Patel AV. Formulating bacterial endophyte: Pre-conditioning of cells and the encapsulation in amidated pectin beads. Biotechnology reports (Amsterdam, Netherlands). 2020;26: e00463.
Cruz Barrera, M., Jakobs-Schönwandt, D., Gomez, M. I., Serrato, J., Ruppel, S., & Patel, A. V. (2020). Formulating bacterial endophyte: Pre-conditioning of cells and the encapsulation in amidated pectin beads. Biotechnology reports (Amsterdam, Netherlands), 26, e00463. doi:10.1016/j.btre.2020.e00463
Cruz Barrera, M., Jakobs-Schönwandt, D., Gomez, M. I., Serrato, J., Ruppel, S., and Patel, A. V. (2020). Formulating bacterial endophyte: Pre-conditioning of cells and the encapsulation in amidated pectin beads. Biotechnology reports (Amsterdam, Netherlands) 26: e00463.
Cruz Barrera, M., et al., 2020. Formulating bacterial endophyte: Pre-conditioning of cells and the encapsulation in amidated pectin beads. Biotechnology reports (Amsterdam, Netherlands), 26: e00463.
M. Cruz Barrera, et al., “Formulating bacterial endophyte: Pre-conditioning of cells and the encapsulation in amidated pectin beads.”, Biotechnology reports (Amsterdam, Netherlands), vol. 26, 2020, : e00463.
Cruz Barrera, M., Jakobs-Schönwandt, D., Gomez, M.I., Serrato, J., Ruppel, S., Patel, A.V.: Formulating bacterial endophyte: Pre-conditioning of cells and the encapsulation in amidated pectin beads. Biotechnology reports (Amsterdam, Netherlands). 26, : e00463 (2020).
Cruz Barrera, Mauricio, Jakobs-Schönwandt, Désirée, Gomez, Martha Isabel, Serrato, Juan, Ruppel, Silke, and Patel, Anant V. “Formulating bacterial endophyte: Pre-conditioning of cells and the encapsulation in amidated pectin beads.”. Biotechnology reports (Amsterdam, Netherlands) 26 (2020): e00463.

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