Aldoxime Dehydratase Mutants as Improved Biocatalysts for a Sustainable Synthesis of Biorenewables-Based 2-Furonitrile

Choi J-E, Shinoda S, Asano Y, Gröger H (2020)
Catalysts 10(4): 362.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Choi, Ji-EunUniBi; Shinoda, Suguru; Asano, Yasuhisa; Gröger, HaraldUniBi
Abstract / Bemerkung
2-Furonitrile is an interesting nitrile product for the chemical industry due to its use as intermediate in the field of fine chemicals and pharmaceuticals or as a potential sweetener, as well as due to its access from biorenewables. As an alternative to current processes based on, e.g., the ammoxidation of furfural with ammonia as a gas phase reaction running at > 400 °C, we recently reported an enzymatic dehydration of 2-furfuryl aldoxime being obtained easily from furfural and hydroxylamine. However, improving the catalytic properties of the aldoxime dehydratase biocatalyst from Rhodococcus sp. YH3-3 (OxdYH3-3) in terms of activity and stability remained a challenge. In this contribution, the successful development of aldoxime dehydratase OxdYH3-3 mutants that were generated by directed evolution and its enhanced activity toward 2-furfuryl aldoxime is reported. The mutant OxdYH3-3 N266S showed an improved activity of up to six times higher than the wild type when utilizing a substrate concentration of 50–100 mM of 2-furfuryl aldoxime.
Stichworte
Physical and Theoretical Chemistry; Catalysis
Erscheinungsjahr
2020
Zeitschriftentitel
Catalysts
Band
10
Ausgabe
4
Art.-Nr.
362
eISSN
2073-4344
Page URI
https://pub.uni-bielefeld.de/record/2943227

Zitieren

Choi J-E, Shinoda S, Asano Y, Gröger H. Aldoxime Dehydratase Mutants as Improved Biocatalysts for a Sustainable Synthesis of Biorenewables-Based 2-Furonitrile. Catalysts. 2020;10(4): 362.
Choi, J. - E., Shinoda, S., Asano, Y., & Gröger, H. (2020). Aldoxime Dehydratase Mutants as Improved Biocatalysts for a Sustainable Synthesis of Biorenewables-Based 2-Furonitrile. Catalysts, 10(4), 362. doi:10.3390/catal10040362
Choi, J. - E., Shinoda, S., Asano, Y., and Gröger, H. (2020). Aldoxime Dehydratase Mutants as Improved Biocatalysts for a Sustainable Synthesis of Biorenewables-Based 2-Furonitrile. Catalysts 10:362.
Choi, J.-E., et al., 2020. Aldoxime Dehydratase Mutants as Improved Biocatalysts for a Sustainable Synthesis of Biorenewables-Based 2-Furonitrile. Catalysts, 10(4): 362.
J.-E. Choi, et al., “Aldoxime Dehydratase Mutants as Improved Biocatalysts for a Sustainable Synthesis of Biorenewables-Based 2-Furonitrile”, Catalysts, vol. 10, 2020, : 362.
Choi, J.-E., Shinoda, S., Asano, Y., Gröger, H.: Aldoxime Dehydratase Mutants as Improved Biocatalysts for a Sustainable Synthesis of Biorenewables-Based 2-Furonitrile. Catalysts. 10, : 362 (2020).
Choi, Ji-Eun, Shinoda, Suguru, Asano, Yasuhisa, and Gröger, Harald. “Aldoxime Dehydratase Mutants as Improved Biocatalysts for a Sustainable Synthesis of Biorenewables-Based 2-Furonitrile”. Catalysts 10.4 (2020): 362.
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