High density cultivation for efficient sesquiterpenoid biosynthesis in Synechocystis sp. PCC 6803.

Dienst D, Wichmann J, Mantovani O, Rodrigues JS, Lindberg P (2020)
Scientific reports 10(1): 5932.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Dienst, Dennis; Wichmann, Julian; Mantovani, Oliver; Rodrigues, Joao S; Lindberg, Pia
Abstract / Bemerkung
Cyanobacteria and microalgae are attractive photoautotrophic host systems for climate-friendly production of fuels and other value-added biochemicals. However, for economic applications further development and implementation of efficient and sustainable cultivation strategies are essential. Here, we present a comparative study on cyanobacterial sesquiterpenoid biosynthesis in Synechocystis sp. PCC 6803 using a commercial lab-scale High Density Cultivation (HDC) platform in the presence of dodecane as in-situ extractant. Operating in a two-step semi-batch mode over a period of eight days, volumetric yields of (E)-alpha-bisabolene were more than two orders of magnitude higher than previously reported for cyanobacteria, with final titers of 179.4±20.7mg * L-1. Likewise, yields of the sesquiterpene alcohols (-)-patchoulol and (-)-alpha-bisabolol were many times higher than under reference conditions, with final titers of 17.3±1.85mg * L-1 and 96.3±2.2mg * L-1, respectively. While specific productivity was compromised particularly for (E)-alpha-bisabolene in the HDC system during phases of high biomass accumulation rates, volumetric productivity enhancements during linear growth at high densities were more pronounced for (E)-alpha-bisabolene than for the hydroxylated terpenoids. Together, this study provides additional insights into cell density-related process characteristics, introducing HDC as highly efficient strategy for phototrophic terpenoid production in cyanobacteria.
Erscheinungsjahr
2020
Zeitschriftentitel
Scientific reports
Band
10
Ausgabe
1
Art.-Nr.
5932
eISSN
2045-2322
Page URI
https://pub.uni-bielefeld.de/record/2942708

Zitieren

Dienst D, Wichmann J, Mantovani O, Rodrigues JS, Lindberg P. High density cultivation for efficient sesquiterpenoid biosynthesis in Synechocystis sp. PCC 6803. Scientific reports. 2020;10(1): 5932.
Dienst, D., Wichmann, J., Mantovani, O., Rodrigues, J. S., & Lindberg, P. (2020). High density cultivation for efficient sesquiterpenoid biosynthesis in Synechocystis sp. PCC 6803. Scientific reports, 10(1), 5932. doi:10.1038/s41598-020-62681-w
Dienst, D., Wichmann, J., Mantovani, O., Rodrigues, J. S., and Lindberg, P. (2020). High density cultivation for efficient sesquiterpenoid biosynthesis in Synechocystis sp. PCC 6803. Scientific reports 10:5932.
Dienst, D., et al., 2020. High density cultivation for efficient sesquiterpenoid biosynthesis in Synechocystis sp. PCC 6803. Scientific reports, 10(1): 5932.
D. Dienst, et al., “High density cultivation for efficient sesquiterpenoid biosynthesis in Synechocystis sp. PCC 6803.”, Scientific reports, vol. 10, 2020, : 5932.
Dienst, D., Wichmann, J., Mantovani, O., Rodrigues, J.S., Lindberg, P.: High density cultivation for efficient sesquiterpenoid biosynthesis in Synechocystis sp. PCC 6803. Scientific reports. 10, : 5932 (2020).
Dienst, Dennis, Wichmann, Julian, Mantovani, Oliver, Rodrigues, Joao S, and Lindberg, Pia. “High density cultivation for efficient sesquiterpenoid biosynthesis in Synechocystis sp. PCC 6803.”. Scientific reports 10.1 (2020): 5932.

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