CRISPR interference-based gene repression in the plant growth promoter Paenibacillus sonchi genomovar Riograndensis SBR5

Fernandes de Brito L, Schultenkämper K, Passaglia L, Wendisch VF (2020)
Applied Microbiology and Biotechnology 104: 5095-5106.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
OA 897.90 KB
Abstract / Bemerkung
Gene repression using the endonucleolytically deactivated dCas9 protein and sgRNAs (CRISPR interference or CRISPRi) is a useful approach to study gene functions. Here, we established CRISPRi in Paenibacillus sonchi genomovar Riograndensis SBR5, a plant growth promoting bacterium. CRISPRi system with sgRNAs targeting SBR5 endogenous genes spo0A, yaaT and ydjJ and plasmid-borne gfpUV was constructed and analyzed. Flow cytometry analysis revealed a significant decrease of reporter protein GFPUV signal in P. sonchi strains expressing gfpUV sgRNA in comparison with non-targeting controls. CRISPRi-based repression of chromosomal genes for regulation of sporulation spo0A and yaaT decreased sporulation and increased biofilm formation in SBR5. Repression of the sorbitol catabolic gene ydjJ revealed decreased specific activity of YdjJ in crude cell extracts and reduced biomass formation from sorbitol in growth experiments. Our work on CRISPRi-based gene repression serves as basis for gene function studies of the plant growth promoter P. sonchi SBR5. To our knowledge, the present study presents the first tool for gene repression established in Paenibacillus species.
Erscheinungsjahr
2020
Zeitschriftentitel
Applied Microbiology and Biotechnology
Band
104
Seite(n)
5095-5106
eISSN
1432-0614
Page URI
https://pub.uni-bielefeld.de/record/2941929

Zitieren

Fernandes de Brito L, Schultenkämper K, Passaglia L, Wendisch VF. CRISPR interference-based gene repression in the plant growth promoter Paenibacillus sonchi genomovar Riograndensis SBR5. Applied Microbiology and Biotechnology . 2020;104:5095-5106.
Fernandes de Brito, L., Schultenkämper, K., Passaglia, L., & Wendisch, V. F. (2020). CRISPR interference-based gene repression in the plant growth promoter Paenibacillus sonchi genomovar Riograndensis SBR5. Applied Microbiology and Biotechnology , 104, 5095-5106. doi:10.1007/s00253-020-10571-6
Fernandes de Brito, L., Schultenkämper, K., Passaglia, L., and Wendisch, V. F. (2020). CRISPR interference-based gene repression in the plant growth promoter Paenibacillus sonchi genomovar Riograndensis SBR5. Applied Microbiology and Biotechnology 104, 5095-5106.
Fernandes de Brito, L., et al., 2020. CRISPR interference-based gene repression in the plant growth promoter Paenibacillus sonchi genomovar Riograndensis SBR5. Applied Microbiology and Biotechnology , 104, p 5095-5106.
L. Fernandes de Brito, et al., “CRISPR interference-based gene repression in the plant growth promoter Paenibacillus sonchi genomovar Riograndensis SBR5”, Applied Microbiology and Biotechnology , vol. 104, 2020, pp. 5095-5106.
Fernandes de Brito, L., Schultenkämper, K., Passaglia, L., Wendisch, V.F.: CRISPR interference-based gene repression in the plant growth promoter Paenibacillus sonchi genomovar Riograndensis SBR5. Applied Microbiology and Biotechnology . 104, 5095-5106 (2020).
Fernandes de Brito, Luciana, Schultenkämper, Kerstin, Passaglia, Luciane, and Wendisch, Volker F. “CRISPR interference-based gene repression in the plant growth promoter Paenibacillus sonchi genomovar Riograndensis SBR5”. Applied Microbiology and Biotechnology 104 (2020): 5095-5106.
Alle Dateien verfügbar unter der/den folgenden Lizenz(en):
Creative Commons Namensnennung 4.0 International Public License (CC-BY 4.0):
Volltext(e)
Access Level
OA Open Access
Zuletzt Hochgeladen
2020-07-21T12:19:25Z
MD5 Prüfsumme
c98ff3b0280607090a01c7190e02751b

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 32274563
PubMed | Europe PMC

Suchen in

Google Scholar