CRISPR interference-based gene repression in the plant growth promoter Paenibacillus sonchi genomovar Riograndensis SBR5

Fernandes de Brito L, Schultenkämper K, Passaglia L, Wendisch VF (2020)
Applied Microbiology and Biotechnology 104: 5095-5106.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Gene repression using the endonucleolytically deactivated dCas9 protein and sgRNAs (CRISPR interference or CRISPRi) is a useful approach to study gene functions. Here, we established CRISPRi in Paenibacillus sonchi genomovar Riograndensis SBR5, a plant growth promoting bacterium. CRISPRi system with sgRNAs targeting SBR5 endogenous genes spo0A, yaaT and ydjJ and plasmid-borne gfpUV was constructed and analyzed. Flow cytometry analysis revealed a significant decrease of reporter protein GFPUV signal in P. sonchi strains expressing gfpUV sgRNA in comparison with non-targeting controls. CRISPRi-based repression of chromosomal genes for regulation of sporulation spo0A and yaaT decreased sporulation and increased biofilm formation in SBR5. Repression of the sorbitol catabolic gene ydjJ revealed decreased specific activity of YdjJ in crude cell extracts and reduced biomass formation from sorbitol in growth experiments. Our work on CRISPRi-based gene repression serves as basis for gene function studies of the plant growth promoter P. sonchi SBR5. To our knowledge, the present study presents the first tool for gene repression established in Paenibacillus species.
Erscheinungsjahr
2020
Zeitschriftentitel
Applied Microbiology and Biotechnology
Band
104
Seite(n)
5095-5106
eISSN
1432-0614
Finanzierungs-Informationen
Open-Access-Publikationskosten wurden durch die Universität Bielefeld im Rahmen des DEAL-Vertrags gefördert.
Page URI
https://pub.uni-bielefeld.de/record/2941929

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Fernandes de Brito L, Schultenkämper K, Passaglia L, Wendisch VF. CRISPR interference-based gene repression in the plant growth promoter Paenibacillus sonchi genomovar Riograndensis SBR5. Applied Microbiology and Biotechnology . 2020;104:5095-5106.
Fernandes de Brito, L., Schultenkämper, K., Passaglia, L., & Wendisch, V. F. (2020). CRISPR interference-based gene repression in the plant growth promoter Paenibacillus sonchi genomovar Riograndensis SBR5. Applied Microbiology and Biotechnology , 104, 5095-5106. doi:10.1007/s00253-020-10571-6
Fernandes de Brito, Luciana, Schultenkämper, Kerstin, Passaglia, Luciane, and Wendisch, Volker F. 2020. “CRISPR interference-based gene repression in the plant growth promoter Paenibacillus sonchi genomovar Riograndensis SBR5”. Applied Microbiology and Biotechnology 104: 5095-5106.
Fernandes de Brito, L., Schultenkämper, K., Passaglia, L., and Wendisch, V. F. (2020). CRISPR interference-based gene repression in the plant growth promoter Paenibacillus sonchi genomovar Riograndensis SBR5. Applied Microbiology and Biotechnology 104, 5095-5106.
Fernandes de Brito, L., et al., 2020. CRISPR interference-based gene repression in the plant growth promoter Paenibacillus sonchi genomovar Riograndensis SBR5. Applied Microbiology and Biotechnology , 104, p 5095-5106.
L. Fernandes de Brito, et al., “CRISPR interference-based gene repression in the plant growth promoter Paenibacillus sonchi genomovar Riograndensis SBR5”, Applied Microbiology and Biotechnology , vol. 104, 2020, pp. 5095-5106.
Fernandes de Brito, L., Schultenkämper, K., Passaglia, L., Wendisch, V.F.: CRISPR interference-based gene repression in the plant growth promoter Paenibacillus sonchi genomovar Riograndensis SBR5. Applied Microbiology and Biotechnology . 104, 5095-5106 (2020).
Fernandes de Brito, Luciana, Schultenkämper, Kerstin, Passaglia, Luciane, and Wendisch, Volker F. “CRISPR interference-based gene repression in the plant growth promoter Paenibacillus sonchi genomovar Riograndensis SBR5”. Applied Microbiology and Biotechnology 104 (2020): 5095-5106.
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2020-07-21T12:19:25Z
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