Mass spectrometry imaging reveals lipid upregulation and bile acid changes indicating amitriptyline induced steatosis in a rat model.

Kampa JM, Sahin M, Slopianka M, Giampa M, Bednarz H, Ernst R, Riefke B, Niehaus K, Fatangare A (In Press)
Toxicology letters 325: 43-50.

Zeitschriftenaufsatz | Im Druck | Englisch
 
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Autor*in
Kampa, Judith MariaUniBi; Sahin, MikailUniBi; Slopianka, Markus; Giampa, MarcoUniBi; Bednarz, HannaUniBi; Ernst, Rainer; Riefke, Bjoern; Niehaus, KarstenUniBi; Fatangare, Amol
Abstract / Bemerkung
As a consequence of the detoxification process, drugs and drug related metabolites can accumulate in the liver, resulting in drug induced liver injury (DILI), which is the major cause for dose limitation. Amitriptyline, a commonly used tricyclic anti-depressant, is known to cause DILI. The mechanism of Amitriptyline induced liver injury is not yet completely understood. However, as it undergoes extensive hepatic metabolism, unraveling the molecular changes in the liver upon Amitriptyline treatment can help understand Amitriptyline's mode of toxicity. In this study, Amitriptyline treated male rat liver tissue was analyzed using Matrix Assisted Laser Desorption/Ionization-Mass Spectrometry Imaging (MALDI-MSI) to investigate the spatial abundances of Amitriptyline, lipids, and bile acids. The metabolism of Amitriptyline in liver tissue was successfully demonstrated, as the spatial distribution of Amitriptyline and its metabolites localize throughout treatment group liver samples. Several lipids appear upregulated, from which nine were identified as distinct phosphatidylcholine (PC) species. The detected bile acids were found to be lower in Amitriptyline treatment group. The combined results from histological findings, Oil Red O staining, and lipid zonation by MSI reveal lipid upregulation in the periportal area indicating drug induced macrovesicular steatosis (DIS). Copyright © 2020. Published by Elsevier B.V.
Erscheinungsjahr
2020
Zeitschriftentitel
Toxicology letters
Band
325
Seite(n)
43-50
ISSN
0378-4274
eISSN
1879-3169
Page URI
https://pub.uni-bielefeld.de/record/2941713

Zitieren

Kampa JM, Sahin M, Slopianka M, et al. Mass spectrometry imaging reveals lipid upregulation and bile acid changes indicating amitriptyline induced steatosis in a rat model. Toxicology letters. In Press;325:43-50.
Kampa, J. M., Sahin, M., Slopianka, M., Giampa, M., Bednarz, H., Ernst, R., Riefke, B., et al. (In Press). Mass spectrometry imaging reveals lipid upregulation and bile acid changes indicating amitriptyline induced steatosis in a rat model. Toxicology letters, 325, 43-50. doi:10.1016/j.toxlet.2020.02.007
Kampa, J. M., Sahin, M., Slopianka, M., Giampa, M., Bednarz, H., Ernst, R., Riefke, B., Niehaus, K., and Fatangare, A. (In Press). Mass spectrometry imaging reveals lipid upregulation and bile acid changes indicating amitriptyline induced steatosis in a rat model. Toxicology letters 325, 43-50.
Kampa, J.M., et al., In Press. Mass spectrometry imaging reveals lipid upregulation and bile acid changes indicating amitriptyline induced steatosis in a rat model. Toxicology letters, 325, p 43-50.
J.M. Kampa, et al., “Mass spectrometry imaging reveals lipid upregulation and bile acid changes indicating amitriptyline induced steatosis in a rat model.”, Toxicology letters, vol. 325, In Press, pp. 43-50.
Kampa, J.M., Sahin, M., Slopianka, M., Giampa, M., Bednarz, H., Ernst, R., Riefke, B., Niehaus, K., Fatangare, A.: Mass spectrometry imaging reveals lipid upregulation and bile acid changes indicating amitriptyline induced steatosis in a rat model. Toxicology letters. 325, 43-50 (In Press).
Kampa, Judith Maria, Sahin, Mikail, Slopianka, Markus, Giampa, Marco, Bednarz, Hanna, Ernst, Rainer, Riefke, Bjoern, Niehaus, Karsten, and Fatangare, Amol. “Mass spectrometry imaging reveals lipid upregulation and bile acid changes indicating amitriptyline induced steatosis in a rat model.”. Toxicology letters 325 (In Press): 43-50.

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